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Analysis of two exonucleases in the 3' end formation of piRNAs

Research Project

Project/Area Number 17K17673
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
System genome science
Research InstitutionThe University of Tokyo

Principal Investigator

Izumi Natsuko  東京大学, 定量生命科学研究所, 助教 (50579274)

Research Collaborator SHOJI Keisuke  
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordspiRNA / RNAサイレンシング / Trimmer / Zucchini / ヌクレアーゼ / トリミング / トランスポゾン
Outline of Final Research Achievements

In this study, we established Trimmer-KO BmN4 cells and investigated biogenesis mechanism of Trimmer substrates, pre-piRNAs in silkworms. We found that both two endo-cleavage pathways by endonuclease BmZuc and PIWI protein act in the production of silkworm pre-piRNAs. Furthermore, we established in vitro assay system to monitor BmZuc activity in the pre-piRNA production and demonstrated that BmZuc cleavage predominantly generates ~35 nt pre-piRNAs by the assistance of RNA helicase, BmArmi.

Academic Significance and Societal Importance of the Research Achievements

piRNAを産生できない動物個体は、生殖巣の形成不全がみられ不妊となることから、生殖細胞におけるpiRNA経路の重要性は明白である。その一方で、どのようにしてpiRNAがつくられるのか、その産生機構については未だ不明な点が多い。本研究はカイコのモデル細胞を用いてpiRNA産生過程の一端を明らかにしており、piRNA産生機構の理解を前進させるものとして学術的意義がある。また、本研究で確立した実験系や技術手法は、今後のpiRNA研究においても役立つことが期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (1 results)

  • [Journal Article] PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development.2018

    • Author(s)
      Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T.
    • Journal Title

      EMBO Rep.

      Volume: - Issue: 3

    • DOI

      10.15252/embr.201744957

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptome profiling reveals infection strategy of an insect maculavirus2018

    • Author(s)
      Katsuma Susumu、Kawamoto Munetaka、Shoji Keisuke、Aizawa Takahiro、Kiuchi Takashi、Izumi Natsuko、Ogawa Moe、Mashiko Takaaki、Kawasaki Hideki、Sugano Sumio、Tomari Yukihide、Suzuki Yutaka、Iwanaga Masashi
    • Journal Title

      DNA Research

      Volume: 印刷中 Issue: 3 Pages: 277-286

    • DOI

      10.1093/dnares/dsx056

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural basis for arginine methylation-independent recognition of PIWIL1 by TDRD2.2017

    • Author(s)
      Zhang H, Liu K, Izumi N, Huang H, Ding D, Ni Z, Sidhu SS, Chen C, Tomari Y, Min J.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 114 Issue: 47 Pages: 12483-12488

    • DOI

      10.1073/pnas.1711486114

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Analysis of 3′-end formation of silkworm piRNAs using Trimmer knockout cell line2018

    • Author(s)
      Natsuko Izumi, Keisuke Shoji, Yukihide Tomari
    • Organizer
      日本RNA学会
    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2020-03-30  

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