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Elucidation of stem cell-specific post-transcriptional regulation

Research Project

Project/Area Number 17K17834
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field System genome science
Medical genome science
Research InstitutionThe University of Tokyo (2018-2019)
Kyoto University (2017)

Principal Investigator

Yokoshi Moe  東京大学, 定量生命科学研究所, 助教 (80791938)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords転写後制御 / 幹細胞 / エピトランスクリプトーム / 再生医療 / mRNA / 転写後調節 / RNA修飾
Outline of Final Research Achievements

In this study, we aimed to establish a molecular basis for the regulation of stem cell-specific RNA methylation. Ribosome Profiling in human iPS and ES cells and human fibroblast (HDF) cells revealed that the genes that are specifically translationally active in stem cells are mainly those involved in RNA metabolism. Furthermore, the results of RNAi screening suggest that genes involved in RNA modification may be important for stem cell function. Therefore, we confirmed the expression level of small RNA, which is expected to be bound by the identified RNA modification-related proteins, was more than 5-fold higher in stem cells than in HDF cells.

Academic Significance and Societal Importance of the Research Achievements

本研究では、翻訳活性型リボソーム結合mRNAとRNAメチル化修飾の解析という新しい二方向のアプローチから統合的に幹細胞特異的な転写後制御機構を明らかにすることを目指しており学術的意義の高い研究内容であると考える。本研究を通じて、幹細胞での転写後制御機構の役割を解明し、将来的に神経難病における幹細胞レベルでの転写後制御機構の異常が同定されれば、mRNA代謝異常で引き起こされる疾患の研究分野全体に大きく貢献でき、社会的にも高い意義を持つものと考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2017

All Presentation (2 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] mRNAの網羅的解析からアプローチする転写後調節機構2017

    • Author(s)
      余越 萌, 河原行郎,齊藤博英
    • Organizer
      第19回 日本RNA学会年会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 幹細胞特異的な転写後制御機構の解明を目指して2017

    • Author(s)
      余越 萌
    • Organizer
      東京大学先端科学技術研究センター システム生物学セミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 神経前駆細胞の選別方法2017

    • Inventor(s)
      高橋淳, 森実飛鳥, 春原匡, 齊藤博英, パーカラム, 余越萌
    • Industrial Property Rights Holder
      高橋淳, 森実飛鳥, 春原匡, 齊藤博英, パーカラム, 余越萌
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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