| Project/Area Number |
17K17879
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
System genome science
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| Research Institution | Osaka University (2018-2019)
Kobe University (2017) |
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Principal Investigator |
Banno Satomi 大阪大学, 情報科学研究科, 特別研究員(PD) (00513160)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 合成生物学 / ゲノム編集 / CRISPR / 大腸菌 / 遺伝子工学 / 微生物 / 生物・生体工学 |
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| Outline of Final Research Achievements |
In this study, I advanced the direct and efficient targeted base-editing tool of genomic DNA in Escherichia coli. Mediated by its deaminase reaction, cell lethality which had been associated with conventional nuclease-mediated genome editing has been avoided. The tool is simple and efficient enough to perform simultaneous editing of 6 different target genes involved in a metabolic pathway, or 4 types of transposase genes comprising 41 loci in total. As this system is not lethal for E. coli cells and thus may be applicable to wide range of bacterial strains, then I believe this study is of great interest for broad community.
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| Academic Significance and Societal Importance of the Research Achievements |
原核生物において従来のCRISPR/Cas9技術による2本鎖切断は致死であることが多く、これまで適用することは困難であった。本研究で創出された原核生物のゲノム編集技術は、ゲノムDNAを切断せずに、自然突然変異と同様のプロセスで、複数の標的遺伝子に同時に変異を導入できるという、非常に優れた手法である。そのため、この技術を今後さらに改良することで、様々な原核生物における遺伝子操作性を飛躍的に高めることが可能となり、非常に強力で有用なツールとなると予想される。
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