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Metagenomic approach for within-host genomic diversity in bacteremia

Research Project

Project/Area Number 17K17933
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory medicine
Bacteriology (including mycology)
Research InstitutionKyushu University

Principal Investigator

Gotoh Yasuhiro  九州大学, 医学研究院, 助教 (20558358)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords遺伝的多様性 / 菌血症 / メタゲノム / 患者内多様性 / ヘテロサイト / ハプロタイプ / メタゲノム解析 / クローン / 細菌ゲノム
Outline of Final Research Achievements

Diagnosis of bacteremia is performed by identification of bacterial pathogen using blood culture technique and single colony isolation. However, the causative bacteria present in the patient are not necessarily single clone. It is possible that some genetic diversity exists, and further changes will be occurred by treatment or host immunity. In this study, we performed population genetics and metagenomic analysis to reveal the genetic diversity of the causative bacteria present in individual patients and their variation in cases of bacteremia.

Academic Significance and Societal Importance of the Research Achievements

菌血症を発症した血液中の病原細菌を集団として捉えて、集団遺伝学的な視点から、その遺伝的多様性を解析するという新しい発想に基づく研究であり、特定の分離菌株のみを対象とする従来の解析では得られなかった菌血症の発症・進展に係わる新たな知見が得られた。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (3 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] Genome sequencing verifies relapsed infection of Helicobacter cinaedi2019

    • Author(s)
      Sawada Osamu、Gotoh Yasuhiro、Taniguchi Takako、Furukawa Shota、Yoshimura Dai、Sasaki Satomi、Shida Haruki、Kusunoki Yoshihiro、Yamamura Tsuyoshi、Furuya Ken、Itoh Takehiko、Horita Tetsuya、Hayashi Tetsuya、Misawa Naoaki
    • Journal Title

      Open Forum Infectious Diseases

      Volume: 印刷中 Issue: 5

    • DOI

      10.1093/ofid/ofz200

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multi-step genomic dissection of a suspected intra-hospital Helicobacter cinaedi outbreak2019

    • Author(s)
      Gotoh Yasuhiro、Taniguchi Takako、Yoshimura Dai、Katsura Keisuke、Saeki Yuji、Hirabara Yasutoshi、Fukuda Mayumi、Takajo Ichiro、Tomida Junko、Kawamura Yoshiaki、Ogura Yoshitoshi、Itoh Takehiko、Misawa Naoaki、Okayama Akihiko、Hayashi Tetsuya
    • Journal Title

      Microbial Genomics

      Volume: 5 Issue: 12 Pages: 1-11

    • DOI

      10.1099/mgen.0.000236

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genomic Characterization of β-Glucuronidase?Positive Escherichia coli O157:H7 Producing Stx2a2018

    • Author(s)
      Ogura Yoshitoshi、Seto Kazuko、Morimoto Yo、Nakamura Keiji、Sato Mitsuhiko P.、Gotoh Yasuhiro、Itoh Takehiko、Toyoda Atsushi、Ohnishi Makoto、Hayashi Tetsuya
    • Journal Title

      Emerging Infectious Diseases

      Volume: 24 Issue: 12 Pages: 2219-2227

    • DOI

      10.3201/eid2412.180404

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 院内感染疑い事例の全ゲノムシーケンスによる超高解像度分子疫学解析2019

    • Author(s)
      後藤恭宏, 谷口喬子, 吉村大, 桂啓介, 佐伯裕二, 平原康寿, 福田真弓, 高城一郎, 小椋義俊, 伊藤武彦, 三澤尚明, 岡山昭彦, 林哲也
    • Organizer
      第13回日本ゲノム微生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 院内感染疑い事例の全ゲノムシーケンスによる超高解像度分子疫学解析2018

    • Author(s)
      後藤恭宏、谷口喬子、吉村大、桂啓介、佐伯裕二、平原康寿、福田真弓、高城一郎、小椋義俊、伊藤武彦、三澤尚明、岡山昭彦、林哲也
    • Organizer
      第12回 日本ゲノム微生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] サーベイランス・アウトブレイクの検出2018

    • Author(s)
      後藤恭宏
    • Organizer
      第278回 ICD講習会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] 院内感染の高精度分子疫学解析2017

    • Author(s)
      後藤恭宏
    • Total Pages
      216
    • Publisher
      医薬ジャーナル社
    • ISBN
      9784753281275
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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