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Producing high mineral nutrition rice by ion-beam mutation and genome editing

Research Project

Project/Area Number 17K17998
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Science in genetics and breeding
Plant nutrition/Soil science
Research InstitutionAkita Prefectural University (2018-2020)
Ishikawa Prefectural University (2017)

Principal Investigator

Masuda Hiroshi  秋田県立大学, 生物資源科学部, 助教 (40605268)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords鉄 / 亜鉛 / 栄養強化 / 変異選抜 / イネ / 植物栄養 / 育種 / 重イオンビーム / ゲノム編集 / 栄養学 / バイオテクノロジー / 育種学 / 遺伝子 / 植物
Outline of Final Research Achievements

If we can produce rice varieties with high iron and zinc nutritional values, we can contribute to solve the iron deficiency anemia and zinc deficiency problems for Japanese and world population. Six generations of Koshihikari mutant lines were grown in the field and selected for their high iron and zinc nutritional values. We also crossed the lines with rice varieties that can be grown in Akita Prefecture and have good eating quality, aiming to produce a new brand of rice for local peoples and farmers. The whole genome sequences of the backcrossed lines between the mutant and the wild type were analyzed by next-generation sequencing. It suggested that the causative gene was located in the area of chromosomal loss in the mutant strain.

Academic Significance and Societal Importance of the Research Achievements

社会的意義:鉄や亜鉛栄養価が高いイネ品種を作出出来れば、主食であるコメ食を介して、不足しがちな日常的に追加摂取出来れば、日本人の鉄欠乏性貧血症や亜鉛欠乏症の改善に貢献できる。長年にわたり、毎日継続しやすく、追加コストも少なくて済む有効な方法になる。また、これによりコメの商品価値を高めれば、米作農家にとっても利益となりうる。
学術的意義:原因遺伝子として、未知遺伝子や鉄・亜鉛栄養への関与が不明な遺伝子がある。これらの鉄・亜鉛栄養への関与が明らかになれば、学術的にも新規性が高い。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (23 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (12 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 11 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Development of a mugineic acid family phytosiderophore analog as an iron fertilizer2021

    • Author(s)
      Motofumi Suzuki, Atsumi Urabe, Sayaka Sasaki, Ryo Tsugawa, Satoshi Nishio, Haruka Mukaiyama, Yoshiko Murata et al
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 1558-1558

    • DOI

      10.1038/s41467-021-21837-6

    • NAID

      120007141554

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] How does rice defend against excess iron? : Physiological and molecular mechanisms.2020

    • Author(s)
      Aung MS and Masuda H.
    • Journal Title

      Front. Plant Sci.

      Volume: 11 Pages: 1102-1102

    • DOI

      10.3389/fpls.2020.01102

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Boosting crop nutrition to improve lives2020

    • Author(s)
      Masuda H, Aung MS.
    • Journal Title

      impact

      Volume: 6 Issue: 6 Pages: 68-69

    • DOI

      10.21820/23987073.2020.6.68

    • Related Report
      2020 Annual Research Report
    • Open Access
  • [Journal Article] Synthesis by OsNAS3 Is Important for Mitigating Iron Excess Stress in Rice.2019

    • Author(s)
      Aung MS, Masuda H, Nozoye T,Kobayashi T, Jeon J-S, An G, Nishizawa NK.
    • Journal Title

      Frontiers in Plant Sciences

      Volume: 10 Pages: 660-660

    • DOI

      10.3389/fpls.2019.00660

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Enhancement of iron acquisition in rice by the mugineic acid synthase gene with ferric iron reductase gene and OsIRO2 confers tolerance in submerged and non-submerged calcareous soils.2019

    • Author(s)
      Masuda H, Aung MS, Kobayashi T, Hamada T, Nishizawa NK.
    • Journal Title

      Frontiers in Plant Sciences

      Volume: 10 Pages: 1179-1179

    • DOI

      10.3389/fpls.2019.01179

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Iron-deficiency response and expression of genes related to iron homeostasis in poplars2018

    • Author(s)
      3.Hiroshi Masuda, May Sann Aung, Keisuke Maeda, Takanori Kobayashi, Naoki Takata, Toru Taniguchi, Naoko Kishi Nishizawa
    • Journal Title

      Journal of Soil Science and Plant Nutrition

      Volume: 64 Issue: 5 Pages: 576-588

    • DOI

      10.1080/00380768.2018.1480325

    • NAID

      210000012015

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] イネの鉄過剰:生理的応答と分子メカニズム2018

    • Author(s)
      メイサンアウン 増田寛志
    • Journal Title

      アグリバイオ

      Volume: 2 Pages: 91-92

    • Related Report
      2018 Research-status Report
  • [Journal Article] Rice HRZ ubiquitin ligases are crucial for response to excess iron2018

    • Author(s)
      Aung MS, Kobayashi T, Masuda H, Nishizawa NK
    • Journal Title

      Physiol. Plant.

      Volume: in press Issue: 3 Pages: 282-296

    • DOI

      10.1111/ppl.12698

    • NAID

      120006942648

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Physiological and transcriptomic analysis of responses to different levels of iron excess stress in various rice tissues, Soil Science and Plant Nutrition2018

    • Author(s)
      May Sann Aung, Hiroshi Masuda, Takanori Kobayashi & Naoko K. Nishizawa
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: in press Issue: 3 Pages: 370-385

    • DOI

      10.1080/00380768.2018.1443754

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A new transgenic rice line exhibiting enhanced ferric iron reduction and phytosiderophore production confers tolerance to low iron availability in calcareous soil.2017

    • Author(s)
      Hiroshi Masuda, Erika Shimoshi, Tatsuro Hamada, Takeshi Senoura, Takanori Kobayashi, May Sann Aung, Yasuhiro Ishimaru, Yuko Ogo, Naoko K. Nishizawa.
    • Journal Title

      PLoS ONE

      Volume: 12(3) Issue: 3 Pages: e0173441-e0173441

    • DOI

      10.1371/journal.pone.0173441

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] The iron-chelate transporter OsYSL9 plays a role in iron2017

    • Author(s)
      Senoura T, Sakashita E, Kobayashi T, Takahashi M, Aung MS, Masuda H, Nakanishi H, Nishizawa NK.
    • Journal Title

      Plant Mol Biol

      Volume: 79 Issue: 4-5 Pages: 1-13

    • DOI

      10.1007/s11103-017-0656-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioenergy grass [Erianthus ravennae (L.) Beauv.] secretes two members of mugineic acid family phytosiderophores which involved in their tolerance to Fe deficiency2017

    • Author(s)
      Nozoye Tomoko、Aung May Sann、Masuda Hiroshi、Nakanishi Hiromi、Nishizawa Naoko K.
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 63 Issue: 6 Pages: 543-552

    • DOI

      10.1080/00380768.2017.1394168

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 鉄・亜鉛栄養価の高いイネの作出に関する研究2019

    • Author(s)
      増田寛志
    • Organizer
      2019年度 日本土壌肥料学会東北支部会福島大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 奨励賞受賞記念講演 鉄・亜鉛栄養価の高いイネの作出に関する研究2019

    • Author(s)
      増田寛志
    • Organizer
      2019年度 日本土壌肥料学会 静岡大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] ポプラの鉄欠乏応答と鉄ホメオスタシス関連遺伝子の発現解析2018

    • Author(s)
      増田寛志、メイサンアウン、前田慶介、小林高範、高田直樹、谷口亨、西澤直子
    • Organizer
      第64回 日本土壌肥料学会 神奈川大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ○増田寛志 メイサンアウン 小林高範 石丸泰寛 高橋美智子 樋口恭子 中西啓仁 西澤直子2018

    • Author(s)
      遺伝子導入により鉄の体内輸送と蓄積能を強化した高鉄米の作出
    • Organizer
      第36回 日本植物細胞分子生物学会 金沢大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 樹木ポプラの鉄欠乏応答と鉄関連遺伝子の発現解析2018

    • Author(s)
      増田寛志
    • Organizer
      第42回日本鉄バイオサイエンス学会学術集会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Iron and zinc biofortification of Koshihikari rice obtained by ion-beam irradiation2018

    • Author(s)
      Hiroshi Masuda, May Sann Aung, Takanori Kobayashi, Hiromi Nakanishi, Naoko K. Nishizawa
    • Organizer
      16th International Symposium on Rice Functional Genomics (ISRFG)
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Iron biofortification in important rice varieties obtained by the introduction of multiple genes or ion-beam irradiation2017

    • Author(s)
      Masuda H, Aung MS, Kobayashi T, Nakanishi H, Nishizawa NK
    • Organizer
      14th International Plant Nutrition Colloquium (IPNC)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 鉄関連遺伝子 Gmferritin、HvNAS1、OsYSL2、IDS3を同時導入した高鉄米の作出2017

    • Author(s)
      増田寛志 MAY SANN AUNG 小林高範 西澤直子
    • Organizer
      土壌肥料学会第63回仙台大会
    • Related Report
      2017 Research-status Report
  • [Book] Iron Biofortification: The Gateway to Overcoming Hidden Hunger. In: Costa de Oliveira A., Pegoraro C., Ebeling Viana V. (eds) The Future of Rice Demand: Quality Beyond Productivity.2020

    • Author(s)
      Hiroshi Masuda, May Sann Aung, Takanori Kobayashi, Naoko K. Nishizawa.
    • Publisher
      Springer
    • ISBN
      9783030375096
    • Related Report
      2019 Research-status Report
  • [Remarks] 研究者ホームページ コメの鉄・亜鉛栄養価を高める研究

    • URL

      https://www.dbp.akita-pu.ac.jp/~masuda/research1.html

    • Related Report
      2020 Annual Research Report
  • [Remarks] 研究者の研究業績

    • URL

      https://www.dbp.akita-pu.ac.jp/~masuda/achivement.html

    • Related Report
      2020 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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