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Development a cloud-based analysis environment that enables bioinformatics analysis for nanopore sequencer in the field

Research Project

Project/Area Number 17K18045
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Genome biology
System genome science
Research InstitutionTokyo University of Agriculture

Principal Investigator

SHIWA Yuh  東京農業大学, 生命科学部, 助教 (00647753)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords微生物ゲノム / バイオインフォマティクス / ナノポアシーケンサー / MinION / Galaxy / ゲノム
Outline of Final Research Achievements

MinION, a nanopore sequencer from Oxford Nanopore Technologies, has increasingly attracted attention as a portable sequencer that is capable of sequencing in the field, and novel applications are expected. In this study, in order to facilitate bioinformatics analysis for non-bioinformatician, we developed an analysis environment on the web-based platform Galaxy so that various tools for Illumina and MinION can be used in GUI. The usage of this tool can be viewed from https://github.com/youyuh48/pitanano-galaxy.

Academic Significance and Societal Importance of the Research Achievements

実験研究者にとってシーケンスデータ取得後のバイオインフォマティクス解析は依然として敷居が高い。本研究では、WebアプリケーションのGalaxyをベースとしたMinION向けのクラウド型解析環境の構築を行った。本解析環境はコンテナ型環境のDocker上で動作し、ユーザはノートパソコン上でGalaxyサーバをDockerコンテナとして起動することで、Webブラウザを利用してグラフィカルな操作系でデータ解析が可能となり、バイオインフォマティクス解析の高度化に資すると期待される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2018 2017 Other

All Presentation (2 results) Remarks (1 results)

  • [Presentation] 作ったツール,どうやってユーザーに届ける? ~ Galaxy,Docker,クラウドを活用する:Galaxy にツールを実装・公開するまでの流れ2018

    • Author(s)
      志波優
    • Organizer
      第7回生命医薬情報学連合大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ワークフロー共有にGalaxyを利用する2017

    • Author(s)
      志波優
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Research-status Report
  • [Remarks] Pitanano Galaxy

    • URL

      https://github.com/youyuh48/pitanano-galaxy

    • Related Report
      2018 Annual Research Report

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Published: 2017-04-28   Modified: 2020-03-30  

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