| Project/Area Number |
17K18065
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Molecular biology
Cell biology
|
|---|
| Research Institution | Saitama Medical University |
|---|
Principal Investigator |
YONEDA Ryoma 埼玉医科大学, 医学部, 助教 (00734881)
|
|---|
| Project Period (FY) |
2017-04-01 – 2021-03-31
|
| Project Status |
Completed (Fiscal Year 2020)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | RNAメチル化 / lncRNA / pncRNA-D / TLS/FUS / m6A / cyclin D1 / CCND1 / m6A修飾 / pncRNA / TLS |
|---|
| Outline of Final Research Achievements |
m6A modification of lncRNA named pncRNA-D regulates cell cycle by controlling the expression of cyclin D1.
RNA is also methylated or acetylated, and this time I focused on the most abundant RNA modification, m6A. pncRNA-D is a long noncoding RNA transcribed from the promoter region of cyclin D1. m6A modification of pncRNA-D altered interaction with RNA binding proteins, and half-life of this RNA. in summary, I discovered m6A modification of pncRNA-D is deeply related with cell cycle and cell growth of cancer cell lines.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究では複数のがん細胞由来の細胞株の増殖を抑えるRNA修飾を発見した。これはつまり、がん細胞間で保存された細胞増殖メカニズムを抑制するRNA修飾であることを意味する。本研究をさらに進めると、多くのがん細胞の増殖を抑えられるRNA断片の作製に繋がることが期待される。
|
