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Regulation of cyclin D1 gene expression by lncRNA transcribed from its promoter region

Research Project

Project/Area Number 17K18065
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Molecular biology
Cell biology
Research InstitutionSaitama Medical University

Principal Investigator

YONEDA Ryoma  埼玉医科大学, 医学部, 助教 (00734881)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsRNAメチル化 / lncRNA / pncRNA-D / TLS/FUS / m6A / cyclin D1 / CCND1 / m6A修飾 / pncRNA / TLS
Outline of Final Research Achievements

m6A modification of lncRNA named pncRNA-D regulates cell cycle by controlling the expression of cyclin D1.
RNA is also methylated or acetylated, and this time I focused on the most abundant RNA modification, m6A. pncRNA-D is a long noncoding RNA transcribed from the promoter region of cyclin D1. m6A modification of pncRNA-D altered interaction with RNA binding proteins, and half-life of this RNA. in summary, I discovered m6A modification of pncRNA-D is deeply related with cell cycle and cell growth of cancer cell lines.

Academic Significance and Societal Importance of the Research Achievements

本研究では複数のがん細胞由来の細胞株の増殖を抑えるRNA修飾を発見した。これはつまり、がん細胞間で保存された細胞増殖メカニズムを抑制するRNA修飾であることを意味する。本研究をさらに進めると、多くのがん細胞の増殖を抑えられるRNA断片の作製に繋がることが期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2021 2020 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Non-coding RNA suppresses FUS aggregation caused by mechanistic shear stress on pipetting in a sequence-dependent manner2021

    • Author(s)
      Hamad Nesreen、Yoneda Ryoma、So Masatomo、Kurokawa Riki、Nagata Takashi、Katahira Masato
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 9523-9523

    • DOI

      10.1038/s41598-021-89075-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Long noncoding RNA pncRNA-D?reduces cyclin D1 gene expression and arrests cell cycle through RNA m6A modification2020

    • Author(s)
      Yoneda Ryoma、Ueda Naomi、Uranishi Kousuke、Hirasaki Masataka、Kurokawa Riki
    • Journal Title

      Journal of Biological Chemistry

      Volume: 295 Issue: 17 Pages: 5626-5639

    • DOI

      10.1074/jbc.ra119.011556

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RNA sequence and length contribute to RNA-induced conformational change of TLS/FUS2020

    • Author(s)
      Hamad Nesreen、Mashima Tsukasa、Yamaoki Yudai、Kondo Keiko、Yoneda Ryoma、Oyoshi Takanori、Kurokawa Riki、Nagata Takashi、Katahira Masato
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 2629-2629

    • DOI

      10.1038/s41598-020-59496-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Arginine methylation of translocated in liposarcoma (TLS) inhibits its binding to long noncoding RNA, abrogating TLS-mediated repression of CBP/p300 activity2018

    • Author(s)
      Cui Wei、Yoneda Ryoma、Ueda Naomi、Kurokawa Riki
    • Journal Title

      Journal of Biological Chemistry

      Volume: 293 Issue: 28 Pages: 10937-10948

    • DOI

      10.1074/jbc.ra117.000598

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] CCND1遺伝子発現におけるlncRNAのm6A修飾の役割2021

    • Author(s)
      米田竜馬、上田奈緒美、浦西洸介、平崎正孝、黒川理樹
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] RNA結合タンパク質TLS/FUSの液-液相分離、繊維化に薬剤が与える効果2019

    • Author(s)
      米田 竜馬、上田 奈緒美、金木 清美、板東 俊和、黒川 理樹
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] CCND1プロモーター領域より転写されるlncRNAによる細胞周期への影響2018

    • Author(s)
      米田竜馬、上田奈緒美、黒川理樹
    • Organizer
      日本分子生物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] TLS and long noncoding RNA transcribed from the promoter of cyclin D1 repress its mRNA expression2017

    • Author(s)
      Ryoma YONEDA, Wei Cui, Naomi Ueda, Riki Kurokawa
    • Organizer
      第43回内藤コンファレンス
    • Related Report
      2017 Research-status Report
  • [Presentation] The effect of m6A modification on lncRNA transcribed from Cyclin D1 promoter2017

    • Author(s)
      Ryoma YONEDA, Wei CUI, Naomi UEDA, and Riki KUROKAWA
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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