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Mechanism of chloroplast division checkpoint release in unicellular algae and generality

Research Project

Project/Area Number 17K18091
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Evolutionary biology
Plant molecular biology/Plant physiology
Research InstitutionKeio University

Principal Investigator

SUMIYA Nobuko  慶應義塾大学, 商学部(日吉), 助教 (80534601)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords単細胞藻 / 細胞周期 / 葉緑体分裂 / サイクリンB / 灰色藻 / 単細胞藻類 / 細胞分裂
Outline of Final Research Achievements

In unicellular algae with only one or two chloroplasts, the cell and chloroplast divide in a coordinated manner. One of the mechanisms for coordinated division is that cytokinesis occurs only after the onset of chloroplast division. To elucidate the molecular mechanism, I addressed the regulation mechanism of cyclin B expression, which is repressed in chloroplast division inhibited cells. The cyclin B expression is regulated by a similar (but not exactly the same) mechanism of that in land plants. I also analyzed whether this mechanism exists in a unicellular alga with 2 to 4 chloroplasts per cell and found the existence of a similar mechanism.

Academic Significance and Societal Importance of the Research Achievements

G2/M期移行のサイクリンBの発現量に依存した制御は真核生物に広く保存されることが知られている。単細胞藻において、その発現量は細胞と葉緑体の分裂の協調機構に深く関与することが示唆されたが、藻類におけるサイクリンB調節機構については陸上植物と類似した機構であることを本研究ではじめて示唆した。また葉緑体の分裂が開始してはじめて細胞が分裂するという、細胞と葉緑体の分裂を協調させる仕組みは細胞あたりの葉緑体の数が1個でない単細胞藻にも保存された仕組みであることを示した。これらの結果は細胞周期進行という観点から藻類と陸上植物を比較するための有用な情報を提供するものである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Invited: 1 results) Book (1 results)

  • [Journal Article] 単細胞藻の細胞と葉緑体の分裂の相互制御2019

    • Author(s)
      墨谷暢子
    • Journal Title

      アグリバイオ

      Volume: 3 Pages: 251-255

    • NAID

      40021921327

    • Related Report
      2018 Research-status Report
  • [Journal Article] Mechanism of coordination between cell and chloroplast division in unicellular algae2018

    • Author(s)
      Sumiya Nobuko
    • Journal Title

      PLANT MORPHOLOGY

      Volume: 30 Issue: 1 Pages: 83-89

    • DOI

      10.5685/plmorphol.30.83

    • NAID

      130007622406

    • ISSN
      0918-9726, 1884-4154
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおけるG2/M期移行2019

    • Author(s)
      墨谷 暢子
    • Organizer
      日本植物学会第83 回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 単細胞紅藻Cyanidioschyzon merolaeにおけるR1R2R3 Myb の機能解析2019

    • Author(s)
      墨谷 暢子
    • Organizer
      日本植物形態学会第 31 回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 灰色藻Cyanophora sudaeの細胞周期進行と葉緑体分裂2018

    • Author(s)
      墨谷暢子
    • Organizer
      日本植物学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 単細胞藻類における細胞分裂と葉緑体分裂の協調機構2017

    • Author(s)
      墨谷暢子
    • Organizer
      日本植物形態学会第29回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] Metabolic Engineering of Cyanidioschyzon merolae. In: Kuroiwa T. et al. (eds) Cyanidioschyzon merolae.2017

    • Author(s)
      Sumiya N., Miyagishima S.
    • Publisher
      Springer, Singapore
    • ISBN
      9789811061004
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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