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Regulation of differentiation and proliferation at osteogenic front by G9a

Research Project

Project/Area Number 17K18196
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Morphological basic dentistry
Research InstitutionTsurumi University

Principal Investigator

Hisashi Ideno  鶴見大学, 歯学部, 助教 (40435699)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsG9a / Runx2 / 骨芽細胞分化 / osteogenic front / Senescence / 細胞増殖 / ヒストンメチル化 / エピジェネティクス
Outline of Final Research Achievements

In this study, we aimed to elucidate how histone methyltransferase G9a integrally regulates the expression of proliferation-related genes in osteoblast progenitors at the osteogenic front. We found that the expression of Runx2 was not altered, but the expression of Runx2-regulated Fgfrs (proliferation-related genes) and Osteocalcin (differentiation marker genes) was repressed in G9a-deficient cells. Both G9a and Runx2 bound to the transcriptional regulatory regions of Fgfrs and Osteocalcin, and endogenous G9a bound to Runx2, indicating that G9a enhanced the transcriptional activation of Runx2.
These results suggest that G9a may be a member of the transcriptional regulatory complex of Runx2, which regulates transcription of genes involved in proliferation and differentiation of progenitor osteoblasts.

Academic Significance and Societal Importance of the Research Achievements

ヒストンメチル化酵素G9aは元来、ヒストン H3、9番目リジン残基(H3K9)のモノメチル化(me1)とジメチル化(me2)を担うヒストン修飾酵素として同定された。さらにヒストン以外の機能性タンパク質のメチル化を担う事も報告されてきた。本研究では、G9aがRunx2による転写複合体に含まれており機能的な役割を担う、ヒストン修飾酵素以外の新たな点が示唆された。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (7 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] G9a is involved in the regulation of cranial bone formation through activation of Runx2 function during development2020

    • Author(s)
      Ideno Hisashi、Nakashima Kazuhisa、Komatsu Koichiro、Araki Ryoko、Abe Masumi、Arai Yoshinori、Kimura Hiroshi、Shinkai Yoichi、Tachibana Makoto、Nifuji Akira
    • Journal Title

      Bone

      Volume: 137 Pages: 115332-115332

    • DOI

      10.1016/j.bone.2020.115332

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] G9aはRunx2の活性化を介してマウス頭蓋の骨形成を制御する2020

    • Author(s)
      出野 尚、小松浩一郎、中島和久、新井嘉則、木村 宏、眞貝洋一、立花 誠、二藤 彰
    • Organizer
      第38回日本骨代謝学会学術集会
    • Related Report
      2020 Annual Research Report
  • [Presentation] G9aはRunx2の機能を調節してマウス頭蓋の骨形成を制御する2019

    • Author(s)
      出野 尚、小松浩一郎、中島和久、新井嘉則、立花 誠、木村 宏、二藤 彰
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒストンメチル化酵素G9aによる骨芽細胞分化におけるRunx2の転写活性化能の制御2018

    • Author(s)
      出野 尚、小松浩一郎、中島和久、新井嘉則、立花 誠、木村 宏、二藤 彰
    • Organizer
      第41回日本分子生物学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Annexin A5 prevents force-mediated bone ridge overgrowth at the enthesis2018

    • Author(s)
      1.Hisashi Ideno, Yoshinori Arai, Koichiro Komatsu, Kazuhisa Nakashima, Satoshi Wada, Teruhito Yamashita, Ernst Pöschl, Bent Brachvogel, Yoichi Ezura, Akira Nifuji
    • Organizer
      第40回米国骨代謝学会
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒストンメチル化酵素G9aはマウス歯胚の増殖と分化を制御する2017

    • Author(s)
      出野 尚、上運天太一、島田明美、寺島達夫、中島和久、友岡康弘、中村芳樹、木村 宏、立花 誠、二藤 彰
    • Organizer
      第35回日本骨代謝学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] H3K9MTase G9a regulates tooth development in mice2017

    • Author(s)
      Ideno H., Kamiunten T., Shimada A., Terashima T., Nakashima K., Tomooka Y., Nakamura Y., Kimura H., Tachibana M., Nifuji A.
    • Organizer
      第39回米国骨代謝学会(ASBMR)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Histone methyltransferase G9a is essential for osteoblastic differentiation and skull bone formation during development2017

    • Author(s)
      Ideno H., Komatsu K., Shimada A., Arai Y., Nakashima K., Tachibana M., Kimura H., Nifuji A.
    • Organizer
      第57回米国細胞生物学会議(ASCB)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2022-01-27  

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