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Detecting combinatorial histone modification dynamics at single nucleosome resolution for key genes in Epithelial-Mesenchymal-Transition

Research Project

Project/Area Number 17K18358
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Nanobioscience
Biophysics
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Chang Jen-Chien  国立研究開発法人理化学研究所, 生命医科学研究センター, 研究員 (10792137)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsnucleosome / histone modification / chromatin state / single-molecule imaging / EMT / enChIP / epigenetics / bivalent promoter / chromatin / ChIP-seq / CRISPR/Cas / Single-molecule imaging
Outline of Final Research Achievements

Each human cell type has a unique morphology and function enabled by the usage of specific combinations of different parts of the genome. This research focused on histone modifications, which is one of many molecular levels that are tightly regulated to maintain a healthy cell status. Multiple histone modifications can co-occur on the same nucleosome and function together; however, conventional method cannot identify the accurate combinatorial histone modification in a heterogeneous cell population. In this research, a highly sensitive single-molecule imaging assay was developed to simultaneously detect multiple histone modifications on single nucleosomes extracted from cells. Moreover, to understand Epithelial-Mesenchymal Transition (EMT), a list of candidate genes with varied chromatin states during EMT was identified for further investigation. The results have been presented in several domestic and international conferences.

Academic Significance and Societal Importance of the Research Achievements

The developed method for measuring single nucleosomes has reached an unprecedented resolution and expanded the toolbox for epigenetics research. Furthermore, the biological process EMT studied here is critical for cancer metastasis. Understanding its mechanism is the basis to improve human health.

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2018 2017

All Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Single-molecule detection of combinatorial histone modifications for key genes in Epithelial-Mesenchymal-Transition2018

    • Author(s)
      Jen-Chien Chang, Ye Liu, Kazuhide Watanabe, Prashanti Jeyamohan, Haruka Yabukami, Yuko Sato, Hiroshi Kimura, Aki Minoda
    • Organizer
      The 56th Annual Meeting of The Biophysical Society of Japan
    • Related Report
      2018 Annual Research Report
  • [Presentation] Single-molecule detection of chromatin states for key genes in Epithelial-Mesenchymal-Transition2018

    • Author(s)
      Jen-Chien Chang, Ye Liu, Kazuhide Watanabe, Prashanti Jeyamohan, Haruka Yabukami, Yuko Sato, Hiroshi Kimura, Aki Minoda
    • Organizer
      The 41st Annual Meeting of MBSJ
    • Related Report
      2018 Annual Research Report
  • [Presentation] Mapping combinatorial epigenetic modifications at single nucleosome resolution2017

    • Author(s)
      Jen-Chien Chang, HarukaYabukami, KazuhideWatanabe, Yuko Sato,Takashi Umehara, Keisuke Fujita, Hiroshi Kimura, Yuichi Taniguchi, Toshio Yanagida, Akiko Minoda
    • Organizer
      Consortium of Biological Sciences / Conbio 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Mapping combinatorial epigenetic modifications at single nucleosome resolution2017

    • Author(s)
      Jen-Chien Chang, HarukaYabukami, KazuhideWatanabe, Yuko Sato,Takashi Umehara, Keisuke Fujita, Hiroshi Kimura, Yuichi Taniguchi, Toshio Yanagida, Akiko Minoda
    • Organizer
      The 12th International Workshop on Advanced Genomics
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mapping combinatorial epigenetic modifications at single nucleosome resolution2017

    • Author(s)
      Jen-Chien Chang, HarukaYabukami, KazuhideWatanabe, Yuko Sato,Takashi Umehara, Keisuke Fujita, Hiroshi Kimura, Yuichi Taniguchi, Toshio Yanagida, Akiko Minoda
    • Organizer
      62nd Annual Meeting of Biophysical Society
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2020-03-30  

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