| Project/Area Number |
17K18363
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General pharmacology
General anatomy (including histology/embryology)
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Ariyoshi Tetsuro 国立研究開発法人理化学研究所, 生命機能科学研究センター, 研究員 (00782103)
|
|---|
| Project Period (FY) |
2017-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | RNA / 技術開発 / イメージング / ゲノム / 蛍光イメージング / mRNA / アプタマー |
|---|
| Outline of Final Research Achievements |
The investigator used Romanesco, a fluorogenic RNA previously developed by the investigator, and succeeded in enhancing the sensitivity in Romanesco-based live-cell RNA imaging. Visualizing endogenous gene transcriptional dynamics at single-cell resolution has been achieved and transcriptional oscillation at single-cell level has been discovered. Simultaneous imaging of gene transcriptional dynamics and genomic DNA structural changes was also successful.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果により遺伝子の発現が一つ一つの細胞の中でどのように変化していくのか計測できる技術基盤が確立された。細胞ごとの遺伝子発現の違いは人体を始めとする複雑な生命構造を形作る基盤となるメカニズムであるので、今後本研究成果の応用により幅広い生命科学分野で様々な発見が生み出されるであろうと期待される。
|
