| Project/Area Number |
17K18386
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
Infectious disease medicine
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Park Eunsil 国立感染症研究所, 獣医科学部, 主任研究官 (90750117)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Bas-Congo virus / endocytosis / viral receptor / host / Bas-Congoウイルス / 広い宿主域 / G蛋白質 / pH依存的融合活性 / 受容体媒介細胞内輸送 / 獣医学 / 人獣共通感染症 / ウイルス / 国際感染症学 |
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| Outline of Final Research Achievements |
Bas-Congo virus (BASV) is a novel rhabdovirus, detected from patients with viral hemorrhagic fever. Since BASV has not been isolated yet, BASV-G expressing VSV pseudotype virus (BASVpv) was prepared. Using this BASVpv, characteristics of BASV were analyzed and the following results were found, i.e., 1) various mammalian cells were susceptible to BASVpv infection, indicating the broad host range of BASV, similar to VSV, 2) BASVpv infection was mediated by BASV-G protein, 3) obvious syncytium formation was induced upon transient low pH treatment of BASV-G expressing cells. Furthermore, it has been indicated that BASVpv entered the cells by both a clathrin-dependent pathway and a clathrin-independent pathway. By viral overlay protein binding assay, a cellular membrane protein was found to reacted with BASV-G protein.
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| Academic Significance and Societal Importance of the Research Achievements |
BASVは遺伝子配列は全長の98%が患者血清中のウイルスRNAから決定されたが、ウイルスは分離されていない。現時点では、BASVpvが唯一ウイルスの感染を模倣できる系である。BASVpvを用いて得られた感染機構や受容体候補蛋白質候補などは感染防御法開発に資する。新興感染症が大流行する中で、ウイルスが分離されない感染症においてこのような性状解析は公衆衛生学的側面から意義がある。
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