New technique for tissue regeneration with induction of immature cells by atmospheric pressure plasma
| Project/Area Number |
17K18774
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plasma science and related fields
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Kataoka Yosky 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (40291033)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 大気圧プラズマ / 広域電子顕微鏡 / 大脳皮質 / 未分化細胞 / 梗塞 / 虚血 / 再生 / 中枢神経 / 幹細胞 / 増殖 / 骨髄 / プラズマ / 再生医療 |
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| Outline of Final Research Achievements |
In this study, we evaluated whether direct irradiation of atmospheric pressure plasma to the adult mammalian cerebral cortex can lead to tissue regeneration by inducing immature cells. In particular, we tried to identify the origin of those immature cells, and to observe the induction manner of immature cells after atmospheric pressure plasma irradiation to the cerebral cortex in rats, using large-scale electron microscopy which realizes comprehensive observation of the tissue microstructure in wider range exceeding a few millimeters. Furthermore, even in cerebral infarction model of rats, it was found that many cells expressing immature cell markers can be induced in the infarction area by plasma irradiation.
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| Academic Significance and Societal Importance of the Research Achievements |
大気圧プラズマを利用した生体内（in vivo）組織再生技術は、現在おこなわれているES細胞やiPS細胞を移植する再生技術とは全く異なり、生体が自然に持つ再生能力を最大限に引き出すものであり、中枢神経系のように複雑な神経回路の再生など、これまで困難であった複雑な組織構築を再現する再生医療を実現できる可能性がある。また、iPS細胞のような遺伝子導入を必要としないため癌化のリスクも小さいと思われ、社会的意義も大きい。
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Systematic diagnostics of the electrical, optical, and physicochemical characteristics of low-temperature atmospheric-pressure helium plasma sources2019
Author(s)
Keigo Takeda, Hiromasa Yamada, Kenji Ishikawa, Hajime Sakakita, Jaeho Kim, Masashi Ueda, Jun-ichiro Ikeda, Yoshihiro Akimoto, Yosky Kataoka, Naoaki Yokoyama, Yuzuru Ikehara, and Masaru Hori
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Journal Title
Journal of Physics D:Applied Physics
Volume: 52
Issue: 16
Pages: 165202-165202
DOI
Related Report
Peer Reviewed
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[Journal Article] Application of autonomic nervous function evaluation to job stress screening.2019
Author(s)
Okawa, N., Kuratsune, D., Koizumi, J., Mizuno, K., Kataoka, Y., Kuratsune, H.
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Journal Title
Heliyon
Volume: 5
Issue: 2
Pages: e01194-e01194
DOI
Related Report
Peer Reviewed
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[Journal Article] A versatile platform technology for recombinant vaccines using non-propagative human parainfluenza virus type 2 vector2019
Author(s)
Ohtsuka J, Fukumura M, Furuyama W, Wang S, Hara K, Maeda M, Tsurudome M, Miyamoto H, Kaito A, Tsuda N, Kataoka Y, Mizoguchi A, Takada A, Nosaka T.
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Journal Title
Scientific Reports
Volume: 9
Issue: 1
Pages: 1-9
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Analysis of age-dependent change of tissue microstructure in mouse cerebral cortex using large-scale electron microscopy.2020
Author(s)
Maeda, M., Suga, M., Eguchi, A., Kume, S., Ueda, C., Kataoka, Y.
Organizer
RIKEN Aging Project Symposium 2020
Related Report
Int'l Joint Research
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[Presentation] Dynamics and roles of NG2-expressing progenitor cells.2019
Author(s)
Kataoka, Y., Tamura, Y., Yamato, M., Kume, S., Takata, K., Eguchi, A., Maeda, M.
Organizer
A3 Foresight Program Meeting 2019
Related Report
Int'l Joint Research
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[Presentation] Metabolic features of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).2019
Author(s)
Yamano, E., Sugimoto, M., Kume, S., Watanabe, Y., Soga, T., Bateman, L., Vernon, SD., Kataoka, Y.
Organizer
The 15th Annual International Conference of the Metabolomics Society
Related Report
Int'l Joint Research
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[Presentation] Multi-modal imaging of glial progenitor cells.2019
Author(s)
Kataoka, Y., Tamura, Y., Kume, S., Maeda, M., Eguchi, A., Suga M.
Organizer
Neuro2019
Related Report
Invited
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[Presentation] Analysis of age-dependent change of tissue microstructure in mouse cerebral cortex using large-scale electron microscopy.2019
Author(s)
Maeda, M., Suga, M., Nakamura, M., Suzuki, K., Hasebe, Y., Kikuchi, N., Yamagichi, Y., Eguchi, A., Kume, S., Matsuzaki, R., Ueda, C., Kataoka, Y.
Organizer
Neuro 2019
Related Report
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[Presentation] Large-scale electron microscopic analysis of change of tissue microstructure in adult rat cerebral cortex after irradiation of atmospheric-pressure plasma.2019
Author(s)
Yamato, M., Tamura, Y., Maeda, M., Eguchi, A., Kume, S., Kataoka Y.
Organizer
Neuro 2019
Related Report
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[Presentation] In vivo imaging of cancer microenvironment using fiber-bundle based micro-endoscope.2019
Author(s)
Danno, S., Nakano, M., Goto, T., Wrabel, A., Sakaue-Sawano, A., Ando, R., Miyawaki, A., Funabiki, K., Kataoka, Y.
Organizer
第60回日本組織細胞化学会総会学術集会・第13回日中合同組織細胞化学セミナー合同大会
Related Report
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[Presentation] Large-scale electron microscopy and imaging big data analysis2018
Author(s)
Kataoka, Y., Kume, S., Yamato, M., Tamura, Y., Eguchi, A., Takata, K., Danno, S., Maeda, M., Hasebe, Y., Yamaguchi, Y., Kikuchi, N., Suga, M.
Organizer
A3 Foresight Program Meeting 2018
Related Report
Int'l Joint Research / Invited
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