| Project/Area Number |
17K18796
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Earth and planetary science and related fields
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Research Collaborator |
ISHIKAWA Naoto
SUGAYA Satoshi
TAKIZAWA Yuko
SASAKI Yoko
TAKANO Yoshinori
OGAWA Nanako
OHKOUCHI Naohiko
TSUCHIYA Masashi
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | アミノ酸 / メチオニン / 水陸境界環境 / 安定同位体比 / 食物連鎖 / エネルギー / 安定同位体 / 栄養段階 |
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| Outline of Final Research Achievements |
In this study, I developed ‘column-coupling method’ for measuring stable isotopic composition of methionine, a very minor but indispensable amino acid, in natural organisms. Moreover, applying this method developed, we established a compound-specific isotope methodology for estimating ‘resource proportion (aquatic X% vs. terrestrial Y%)’ and ‘trophic position’ of organisms in food webs where both phytoplankton and terrestrial-plants are potentially utilized as primary resources for consumer species along food chains.
The results including the method and methodology were published as a paper in Limnology and oceanography: Methods, in 2018.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により，水陸境界環境において，有機物資源の水陸混合率，生物の栄養段階，そして，アミノ酸の代謝量（エネルギー消費量）等を定量的に見積る方法論が提案されたことで，地球化学や生態学の分野で，今後，水陸境界環境の研究が進むと期待される。
また本研究で開発した「カラムカップリング法」は，夾雑物の多い試料に，少量含まれている有機化合物の安定同位体比測定（例えば，スポーツ競技におけるドーピング，危険薬物の起源・使用量の推定や，隕石中有機物の起源・生成プロセスの研究）にも直接応用できるため，本研究の結果は，これらの研究に新たな展開をもたらすと期待される。
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