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Development of super-resolving microscope using stimulated emission suppression phenomenon in soft X-ray emission

Research Project

Project/Area Number 17K19021
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Nano/Micro science and related fields
Research InstitutionTohoku University

Principal Investigator

Ejima Takeo  東北大学, 多元物質科学研究所, 准教授 (80261478)

Co-Investigator(Kenkyū-buntansha) 東口 武史  宇都宮大学, 工学部, 教授 (80336289)
若山 俊隆  埼玉医科大学, 保健医療学部, 教授 (90438862)
Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords誘導放出抑制 / 軟X線 / シンチレーター / STED / 超解像 / ナノ / 高空間分解能 / 軟X線顕微鏡 / 2次元検出器 / 誘導発光抑制現象 / 全発光収量 / 軟X線励起STED / ベクトルビーム / 水の窓
Outline of Final Research Achievements

In this study, we investigated scintillators that show high luminescence intensity in the soft X-ray (SX) region from 300 eV to 1.3 keV because luminosities of the scintillators were unknown in the SX region. One of the scintillators, Ce: LSO, was used for the experiments because of the high luminosity. The scintillator irradiated by SX lights exhibited a STED phenomenon. The luminescence area was narrowed as the increase of the intensity ratio of the STED light to the luminescence one when the STED light used was a vector-polarized light. For the experiments, we also developed a tunable laser with high repetition, an achromatic vector polarization optical system, and a super-resolution microscope that combines these instruments. The developed microscope can be applied as a radiographic X-ray microscope or as a two-dimensional detector with high resolution in the SX region.

Academic Significance and Societal Importance of the Research Achievements

今回得られた成果は、生物試料の蛍光染色試料に応用されたSTED顕微鏡と同様に、発光点を掃引することによりX線の2次元検出が可能であることを示している。またシンチレーター自体に試料を載せて軟X線照射することで、いわゆるレントゲン写真と同様に動作する顕微鏡としても働く。シンチレーターは目的と動作する光子エネルギーに応じてさまざまな種類があり、数eVから数十keVの光で発光する。本成果では軟X線領域の光だけを強調しているが、紫外線領域からⅩ線領域まで動作する高い解像度を持つ2次元検出器として動作することが期待できる。また動作波長を変える事により、広く軽元素素材、生物などのナノ構造体に応用できる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (37 results)

All 2020 2019 2018 2017

All Journal Article (18 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 18 results,  Open Access: 10 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Demonstration of stimulated emission depletion phenomenon in luminescence of solid-state scintillator excited by soft X-rays2020

    • Author(s)
      Ejima Takeo、Wakayama Toshitaka、Shinozaki Natsumi、Shoji Misaki、Hatayama Genta、Higashiguchi Takeshi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-020-62100-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Luminescence properties of scintillators in soft X-ray region2020

    • Author(s)
      Ejima Takeo、Kurosawa Shunsuke、Yamaji Akihiro、Hatano Tadashi、Wakayama Toshitaka、Higashiguchi Takeshi、Kitaura Mamoru
    • Journal Title

      Journal of Luminescence

      Volume: 219 Pages: 116850-116850

    • DOI

      10.1016/j.jlumin.2019.116850

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Soft X-ray spectral analysis of laser produced molybdenum plasmas using the fundamental and second harmonics of a Nd:YAG laser2019

    • Author(s)
      Lokasani Ragava、Kawasaki Hiromu、Shimada Yuta、Shoji Misaki、Anraku Kyoya、Ejima Takeo、Hatano Tadashi、Jiang Weihua、Namba Shinichi、Nikl Jan、Zeman Michal、O’Sullivan Gerry、Higashiguchi Takeshi、Limpouch Jiri
    • Journal Title

      Optics Express

      Volume: 27 Issue: 23 Pages: 33351-33358

    • DOI

      10.1364/oe.27.033351

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Supercontinuum vector beam generation by independent manipulations of angular polarization and geometric phase2019

    • Author(s)
      Wakayama Toshitaka、Shinozaki Natsumi、Shoji Misaki、Ishiyama Takayuki、Sakaue Kazuyuki、Miura Taisuke、Ejima Takeo、Higashiguchi Takeshi
    • Journal Title

      Applied Physics Letters

      Volume: 114 Issue: 23 Pages: 231101-231101

    • DOI

      10.1063/1.5093189

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhancement of water-window soft x-ray emission from laser-produced Au plasma under low-pressure nitrogen atmosphere2019

    • Author(s)
      John Christian、Kishimoto Maki、Johzaki Tomoyuki、Higashiguchi Takeshi、Kakunaka Noboru、Matsumoto Yasuhiro、Hasegawa Noboru、Nishikino Masaharu、Ejima Takeo、Sunahara Atsushi、Endo Takuma、Namba Shinichi
    • Journal Title

      Optics Letters

      Volume: 44 Issue: 6 Pages: 1439-1442

    • DOI

      10.1364/ol.44.001439

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quantitative Distribution of DNA, RNA, Histone and Proteins Other than Histone in Mammalian Cells, Nuclei and a Chromosome at High Resolution Observed by Scanning Transmission Soft X-Ray Microscopy (STXM)2019

    • Author(s)
      K. Shinohara, S. Tone, T. Ejima, T. Ohigashi, A. Ito
    • Journal Title

      Cells

      Volume: 8 Issue: 2 Pages: 164-164

    • DOI

      10.3390/cells8020164

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of Apoptotic Nuclei with Infrared Spectra-Microscopy2018

    • Author(s)
      M. Kado, T. Ejima, T. Tone, K. Shinohara
    • Journal Title

      SPring-8/SACLA Research Report

      Volume: 6 Issue: 1 Pages: 35-37

    • DOI

      10.18957/rr.6.1.35

    • NAID

      130007969489

    • ISSN
      2187-6886
    • Year and Date
      2018-01-25
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of Soft X-Ray Microscope in Water Window Using Laser-Produced Plasma Light Source2018

    • Author(s)
      Ejima T.、Kondo Y.、Ono Y.、Dinh T. -H.、Higashiguchi T.、Hatano T.
    • Journal Title

      ICXRL 2016: X-Ray Lasers 2016

      Volume: 2016 Pages: 395-399

    • DOI

      10.1007/978-3-319-73025-7_59

    • ISBN
      9783319730240, 9783319730257
    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Evaluation of a Flat-Field Grazing Incidence Spectrometer for Highly Charged Ion Plasma Emission in 1-10 nm2018

    • Author(s)
      Kondo Y.、Dinh T.-H.、Tamura T.、Ohta S.、Kitano K.、Ejima T.、Hatano T.、Higashiguchi T.
    • Journal Title

      ICXRL 2016: X-Ray Lasers 2016

      Volume: 2016 Pages: 409-412

    • DOI

      10.1007/978-3-319-73025-7_62

    • ISBN
      9783319730240, 9783319730257
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of laboratory-type soft X-ray microscope, CXRM, in Water-window using LPP light source2018

    • Author(s)
      Ejima Takeo、Ono Yuichi、Ito Keisuke、Kawasaki Hiromu、Higashiguchi Takeshi、Tone Shigenobu、Hatano Tadashi
    • Journal Title

      Microscopy and Microanalysis

      Volume: 24 Issue: S2 Pages: 214-215

    • DOI

      10.1017/s1431927618013417

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantum Efficiency of Back-illuminated CMOS Sensor with 1.4×1.4 μm2 Pixel Size2018

    • Author(s)
      Ejima Takeo、Hatano Tadashi
    • Journal Title

      Microscopy and Microanalysis

      Volume: 24 Issue: S2 Pages: 322-323

    • DOI

      10.1017/s1431927618013922

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influence of short pulse duration of carbon dioxide lasers on extreme ultraviolet emission from laser-produced plasmas2018

    • Author(s)
      Amano Reiho、Dinh Thang-Hung、Sasanuma Atsushi、Arai Goki、Hara Hiroyuki、Fujii Yusuke、Hatano Tadashi、Ejima Takeo、Jiang Weihua、Sunahara Atsushi、Takahashi Akihiko、Nakamura Daisuke、Okada Tatsuo、Sakaue Kazuyuki、Miura Taisuke、O’Sullivan Gerry、Higashiguchi Takeshi
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 57 Issue: 7 Pages: 070311-070311

    • DOI

      10.7567/jjap.57.070311

    • NAID

      210000149245

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characteristics of soft x-ray and extreme ultraviolet (XUV) emission from laser-produced highly charged rhodium ions2018

    • Author(s)
      Barte Ellie Floyd、Hara Hiroyuki、Tamura Toshiki、Gisuji Takuya、Chen When-Bo、Lokasani Ragava、Hatano Tadashi、Ejima Takeo、Jiang Weihua、Suzuki Chihiro、Li Bowen、Dunne Padraig、O'Sullivan Gerry、Sasaki Akira、Higashiguchi Takeshi、Limpouch Ji??
    • Journal Title

      Journal of Applied Physics

      Volume: 123 Issue: 18 Pages: 183301-183301

    • DOI

      10.1063/1.5024344

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Selection of target elements for laser-produced plasma soft x-ray sources2018

    • Author(s)
      Tamura Toshiki、Arai Goki、Kondo Yoshiki、Hara Hiroyuki、Hatano Tadashi、Ejima Takeo、Jiang Weihua、Suzuki Chihiro、O’Sullivan Gerry、Higashiguchi Takeshi
    • Journal Title

      Optics Letters

      Volume: 43 Issue: 9 Pages: 2042-2042

    • DOI

      10.1364/ol.43.002042

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cr/Sc/Mo multilayer for condenser optics in water window microscopes2017

    • Author(s)
      T. Hatano, T. Ejima, T. Tsuru
    • Journal Title

      J. Electron Spectrosc. Relat. Phenom.

      Volume: 220 Pages: 14-16

    • DOI

      10.1016/j.elspec.2016.12.010

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Organelle Distribution in a Hydrated Bio-cell by Correlation between Soft X-ray and Fluorescence Images2017

    • Author(s)
      T. Ejima, M. Kado, M. Aoyama, K. Yasuda, and S. Tamotsu
    • Journal Title

      J. Phys.: Conf. Series

      Volume: 849 Pages: 012009-012009

    • DOI

      10.1088/1742-6596/849/1/012009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] ncrease of the emission of laser-produced plasmas under N2 gas atmosphere in the 2.9-6 nm region2017

    • Author(s)
      M. Kado, M. Kishimoto, K. Shinohara, and T. Ejima
    • Journal Title

      Appl. Phys. Lett.

      Volume: 111 Issue: 5

    • DOI

      10.1063/1.4996890

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of the determined vectorial vortex beams by use of an achromatic axially symmetric waveplate2017

    • Author(s)
      Toshitaka Wakayama, Takeshi Higashiguchi, and Yukitoshi Otani
    • Journal Title

      Optical Review

      Volume: 印刷中 Issue: 3 Pages: 449-461

    • DOI

      10.1007/s10043-017-0315-1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 軟X線励起蛍光におけるSX-STEDイメージング2020

    • Author(s)
      江島 丈雄、若山 俊隆、石山 貴之、安楽 響哉、金田 凌祐、ロイ ショミット、津田 準一、坂上 和之、東口 武史
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ビスマスプラズマ水の窓軟X線源の開発2019

    • Author(s)
      川崎 太夢、島田 悠太、難波 慎一、佐々木 明、錦野 将元、羽多野 忠、江島 丈雄、東口 武史
    • Organizer
      電気学会研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 生物細胞観測のための軟X線相関顕微法の開発と現状2019

    • Author(s)
      江島丈雄
    • Organizer
      日本物理学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ce:LSOの軟X線励起蛍光におけるSTED現象2019

    • Author(s)
      江島 丈雄、若山 俊隆、篠崎 夏美、庄司 美咲、石山 貴之、坂上 和之、東口 武史
    • Organizer
      第80回応用物理学会秋季学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] BiターゲットLPP光源を用いた軟X線顕微鏡CXRM2018

    • Author(s)
      江島丈雄、小野祐一、伊藤圭祐、川崎大夢、羽多野忠、刀祢重信、東口武史
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] SX-STED用シンチレーターの探索2018

    • Author(s)
      江島丈雄、黒澤駿介、山路晃広、羽多野忠、若山俊隆、東口武史、北浦守
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] ピクセルサイズ1.4×1.4μm2の背面照射型軟Ⅹ線撮像素子の量子効率2018

    • Author(s)
      江島丈雄、羽多野 忠
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2018 Research-status Report
  • [Presentation] Quantum efficiency of back-illuminated CMOS sensor with 1.4×1.4 µm2 pixel size2018

    • Author(s)
      Takeo EJIMA and Tadashi HATANO
    • Organizer
      14th International conference on x-ray microscopy
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of laboratory-type soft X-ray microscope, CXRM, in Water-window using LPP light source2018

    • Author(s)
      Takeo Ejima, Yuichi Ono, Keisuke Ito, Hiromu Kawasaki, Takeshi Higashiguchi, Shigenobu Tone and Tadashi Hatano
    • Organizer
      14th International conference on x-ray microscopy
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Biプラズマ光源水の窓軟X線顕微鏡のための Cr/Sc/Mo 多層膜コンデンサーミラーの開発2017

    • Author(s)
      羽多野忠、江島丈雄、小野祐一、陳文博、近藤芳希、Thanh-Hung Dinh、田村賢紀、宜寿次拓弥、原広行、荒居剛己、東口武史
    • Organizer
      Ⅹ線結像光学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] ピクセルサイズ 1.4×1.4μm2の軟 X 線撮像素子の開発2017

    • Author(s)
      江島丈雄、羽多野忠
    • Organizer
      Ⅹ線結像光学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 軟X線発光における誘導放出現象を用いた軟X線STED顕微鏡2017

    • Author(s)
      江島丈雄
    • Organizer
      広島大学第513回物性セミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 軟X線発光における誘導放出現象を用いた軟X線STED顕微鏡の可能性2017

    • Author(s)
      江島丈雄
    • Organizer
      第26回バイオイメージング学会学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] ピクセルサイズ 1.4×1.4μm2の軟 X 線撮像素子の開発2017

    • Author(s)
      江島丈雄、羽多野忠
    • Organizer
      第78回応用物理学会秋季学術講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] 企画説明 -テンダーⅩ線領域と微量元素―2017

    • Author(s)
      江島丈雄、白川仁、原田昌彦
    • Organizer
      第28回日本微量元素学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Heガス中レーザー生成プラズマからの8-30nm軟Ⅹ線発光強度2017

    • Author(s)
      江島丈雄、山木賢一
    • Organizer
      第64回応用物理学会春季学術講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] N2ガス中レーザー生成プラズマからの「水の窓」軟Ⅹ線発光強度2017

    • Author(s)
      江島丈雄、岸本牧, 篠原邦夫, 加道雅孝, 小田切誠,角中昇, 難波愼一
    • Organizer
      第64回応用物理学会春季学術講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] ピクセルサイズ 1.4×1.4μm2の軟 X 線撮像素子の開発2017

    • Author(s)
      江島丈雄
    • Organizer
      第30回日本放射光学会年会
    • Related Report
      2017 Research-status Report
  • [Book] X-Ray Lasers 2016, Chap. 592017

    • Author(s)
      T. Ejima, Y. Kondo, Y. Ono, T.-H. Dinh, T. Higashiguchi, and T. Hatano
    • Publisher
      Springer-Verlag
    • ISBN
      9783319730240
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2021-02-19  

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