Novel methodology in cryobiology "superflash freezing"
| Project/Area Number |
17K19028
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nano/Micro science and related fields
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 超瞬間凍結 / 凍結保存 / 低温生物学 / 凍結保護剤フリー / 瞬間凍結 / 細胞保存 |
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| Outline of Final Research Achievements |
All the current methods require at least one cryoprotectant agent (CPA) to ensure water around the cells vitrifies not to form ice crystals, which can damage cells. To the best of our knowledge, a CPA-free cell cryopreservation method has not been established. Here, we demonstrated CPA-free cryopreservation by ultrarapid cooling using inkjet printing.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，世界で初めての凍結保護剤不要の動物細胞凍結保存を，インクジェット技術を利用した超瞬間凍結により実現した．本研究は，主に研究に用いられる3種類の細胞において実証したが，今後はより多くの細胞への適用が望まれる。特に、凍結保護剤の影響が懸念されるヒトiPS細胞やその分化細胞、凍結保護剤の添加が望ましくない生殖医療における精子や卵子，輸血用の血液細胞などが挙げられ、臨床医療を含めた多くの医学および生物学分野への応用が期待される．
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Report
(4 results)
Research Products
(13 results)
