Photo-triggered translation using caged aminoacyl-tRNA in early embryos
| Project/Area Number |
17K19202
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry and related fields
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| Research Institution | Okayama University |
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Principal Investigator |
Ohtsuki Takashi 岡山大学, ヘルスシステム統合科学研究科, 教授 (80321735)
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| Co-Investigator(Kenkyū-buntansha) |
若井 拓哉 岡山大学, 環境生命科学研究科, 准教授 (60557768)
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | ケージドアミノアシルtRNA / 翻訳 / 光制御 / 発生 |
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| Outline of Final Research Achievements |
Light is one of the most easily manipulated external factors for spatiotemporally controlling biological events, such as protein biosynthesis. We synthesized a (7-diethylaminocoumarin-4-yl)methoxycarbonyl (DEACM)-caged aminoacyl-tRNAs (aa-tRNAs) for photocontrolling protein biosynthesis. Upon irradiation at 400-430 nm, DEACM-aa-tRNA was rapidly converted into active aa-tRNA. Translation was photoinduced when DEACM-aa-tRNAs carrying a CUA anticodon were used together with mRNAs harboring a UAG amber codon. Protein synthesis was phototriggered not only in an in vitro translation system, but also in mammalian cells. We tried to apply this strategy to photo-trigger synthesis of a fluorescent protein and PGC-1alpha in a mouse embryonic cell.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で行ったことは、特定のタンパク質の合成（発現）を時空間的に制御する方法の開発である。マウス胚において、効率よく特異的な発現をさせるためには、まだ改善を要することが分かったが、胚を含めて動物個体や三次元的な細胞集合体に適用可能になれば、“時間的”翻訳制御と“空間的”翻訳制御の双方の側面を生かして、様々なタンパク質の役割の解明が可能になる。また、光による時空間的タンパク質発現制御を通じて、初期発生事象の研究および人為的コントロールも可能になると期待される。
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Report
(3 results)
Research Products
(11 results)
