Reactive sulfur metabolomics for the study of cellular sulfur dynamics
| Project/Area Number |
17K19205
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomolecular chemistry and related fields
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| Research Institution | Kumamoto University |
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Principal Investigator |
Sawa Tomohiro 熊本大学, 大学院生命科学研究部(医), 教授 (30284756)
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| Research Collaborator |
Ono Katsuhiko 熊本大学, 大学院生命科学研究部, 助教 (80573592)
Tsutsuki Hiroyasu 熊本大学, 大学院生命科学研究部, 助教 (40586608)
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 活性イオウ / メタボロミクス / 酸化ストレス / システインパースルフィド / 安定同位体 |
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| Outline of Final Research Achievements |
Cysteine persulfide is an L-cysteine derivative having one additional sulfur atom bound to a cysteinyl thiol group, and it serves as a reactive sulfur species that regulates redox homeostasis in cells. In this study, we established a rapid and efficient method of synthesis of L-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome. We used bacterial cysteine syntheses to incorporate isotopic sulfur atoms into the sulfhydryl moiety of L-cysteine. The approach using isotopic sulfur labeling combined with mass spectrometry may thus contribute to greater understanding of reactive sulfur metabolome and redox biology. We further successfully synthesized chemical sulfur donors that efficiently donate sulfane sulfur atoms to acceptor cells.
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| Academic Significance and Societal Importance of the Research Achievements |
システインパースルフィドの代謝は、システインパースルフィド合成酵素の存在も含めて、その分子実態については不明な点も多い。パースルフィドに由来するイオウ原子のユニークな特徴は、チオール分子の間で容易にそのイオウ原子の受渡しを行なうことである（イオウ転移）。このように細胞内でパースルフィドのイオウ代謝は極めてダイナミックである。本研究では、細胞内での多彩なイオウ代謝を網羅的に解析する上で極めて有用な活性イオウメタボロミクスを構築した。今回合成した活性イオウドナーと組み合わせて、詳細なイオウ代謝プロセスを明らかにし、その生理・病態生理研究が進むことが期待される。
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Polysulfide stabilization by tyrosine and hydroxyphenyl-containing derivatives that is important for a reactive sulfur metabolomics analysis.2019
Author(s)
Hamid HA, Tanaka A, Ida T, Nishimura A, Matsunaga T, Fujii S, Morita M, Sawa T, Fukuto JM, Nagy P, Tsutsumi R, Motohashi H, Ihara H, Akaike T.
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Journal Title
Redox Biol
Volume: 21
Pages: 101096-101096
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Reactive sulfur species regulate tRNA methylthiolation and contribute to insulin secretion.2017
Author(s)
Takahashi N, Wei FY, Watanabe S, Hirayama M, Ohuchi Y, Fujimura A, Kaitsuka T, Ishii I, Sawa T, Nakayama H, Akaike T, Tomizawa K.
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Journal Title
Nucleic Acids Res.
Volume: 45
Issue: 1
Pages: 435-445
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Quantitative determination of polysulfide in albumins, plasma proteins and biological fluid samples using a novel combined assays approach2017
Author(s)
keda, M. Ishima, Y. Shibata, A. Chuang, V. T. G. Sawa, T. Ihara, H. Watanabe, H. Xian, M. Ouchi, Y. Shimizu, T. Ando, H. Ukawa, M. Ishida, T. Akaike, T. Otagiri, M. Maruyama, T.
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Journal Title
Analytica Chimica Acta
Volume: 969
Pages: 18-25
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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