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Development of a peptide selection system to modulate polymerase functions

Research Project

Project/Area Number 17K19212
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Biomolecular chemistry and related fields
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Tagami Shunsuke  国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (40586939)

Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsペプチド / ポリメラーゼ / RNAポリメラーゼ / 試験管内進化 / バイオテクノロジー / 生体分子
Outline of Final Research Achievements

In this study, we attempted to develop a peptide selection system to convert polymerases into nucleases. For this purpose, we modified the mRNA display method and realized screening in which peptides were recovered only when a nuclease reaction occurred. We aim to obtain useful peptides by applying this screening system to various polymerases in the future.

Academic Significance and Societal Importance of the Research Achievements

様々な病原体(ウィルス,がん細胞など)の増殖にはその遺伝情報を増幅するためのポリメラーゼが必要です.本研究ではそのようなポリメラーゼの機能を変換し,むしろ病原体の遺伝情報を破壊するようにするためのペプチド開発を進めています.研究期間中に複雑なペプチドスクリーニング法の改変を行いましたが,今後これを利用して様々な有用ペプチドの取得を目指しています.

Report

(3 results)
  • 2021 Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (1 results)

All 2017

All Presentation (1 results)

  • [Presentation] Peptide engineering for therapeutic applications2017

    • Author(s)
      Shunsuke Tagami
    • Organizer
      The 4th RIKEN / Karolinska Institutet / SciLifeLab Joint Symposium
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2023-01-30  

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