| Project/Area Number |
17K19221
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Agricultural chemistry and related fields
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Okada Yohei 東京農工大学, 工学(系)研究科(研究院), 助教 (80749268)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | レドックス応答型色素 / 核酸脱メチル化 |
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| Outline of Final Research Achievements |
Although their molecular weights are several tens or hundreds of thousands, the functions of biomolecules such as nucleic acids and proteins are regulated by the presence of a minimal "methyl" group. In this study, we focused on the development of novel fluorometric dyes that can detect such a small structural difference, which was otherwise difficult to discriminate. As a result, several water soluble dyes constructed with a single benzene were realized.
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| Academic Significance and Societal Importance of the Research Achievements |
核酸やタンパク質などの生体分子のメチル化ならびに脱メチル化は様々な疾病との関連が報告されており，これらの修飾を受けた生体分子や，これらの修飾を触媒する酵素の研究開発は生命科学や医学領域にとって極めて重要な課題となっている．これらの領域における現代の研究には生体分子の蛍光標識が必要不可欠な技術であり，目的や用途に応じた色素の開発が求められている．本研究成果は蛍光標識技術の発展に貢献し得るものである．
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