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Spatiotemporal control of abscisic acid signaling by artificial photoreceptors

Research Project

Project/Area Number 17K19226
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural chemistry and related fields
Research InstitutionShizuoka University

Principal Investigator

Takeuchi Jun  静岡大学, 農学部, 助教 (00776320)

Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords植物 / 有機化学 / 生理活性
Outline of Final Research Achievements

Signaling by the plant stress hormone abscisic acid (ABA) is triggered by activation of PYR/PYL/RCAR receptors (PYLs). By binding to PYLs, ABA induces a conformational change associated with gate closure that enables the receptor to bind to, and inhibit, group-A protein phosphatases 2C (PP2Cs), which normally repress ABA signaling as a negative regulator. Based on this mechanism for activating the receptor, we predicted that a PYL ligand capable of switching the conformation between the gate-opened and the gate-closed forms in response to light can activate or inactivate PYLs by light irradiation. To test this prediction, LIAP/iLIAP, in which the planar vinyl methyl portion of ABA was replaced with an azobenzene group, was designed and synthesized as a photoswitchable PYL agonist/antagonist.

Academic Significance and Societal Importance of the Research Achievements

人を対象とした創薬研究においては,リガンドの活性(構造変化)を光で制御した例は報告されていたが,それを植物ホルモンに適用した例はこれまでなかった。本研究で合成したLIAP/iLIAPは365 nmの光照射により90%以上がZ体(LIAP)となり,ABAと比較すると完全ではないものの,PYLの配座を活性型にするアゴニストとして機能した。また,Z体は自然条件下で徐々に安定型であるE体(iLIAP)に戻っていき,E体はPYLの配座を不活性型にするアンタゴニストとして作用した。これらの知見は,植物ホルモン受容体の活性を光によってON/OFF制御する技術基盤の構築に大きく貢献するものである。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (1 results)

All 2018

All Presentation (1 results)

  • [Presentation] 配座制限型ABA受容体アンタゴニストPAO4の構造展開2018

    • Author(s)
      竹内 純
    • Organizer
      植物化学調節学会第53回大会
    • Related Report
      2018 Research-status Report

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Published: 2017-07-21   Modified: 2021-02-19  

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