| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Outline of Final Research Achievements |
We aimed to construct a membrane potential regulatory system in plant cells by using light-dependent ion transport of the microbial rhodopsin. Six microbial rhodopsin were transiently expressed as fusion proteins with the fluorescent protein Venus in cultured plant cells. We observed the plasma membrane localization of the expressed protein in the outward-facing sodium pump KR2, the cation channel GtCCR4, and the anion channel GtACR1. Light-dependent hyperpolarization of the membrane potential was observed in cells expressing KR2 and GtCCR4 upon exposure to 530 nm light. We further characterized the molecular properties of the cation channel GtCCR4 and the inward-facing proton pump SzR as novel microbial rhodopsins.
|
