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Host-microbe symbiosis mediated through symbiogenic factors

Research Project

Project/Area Number 17K19231
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural chemistry and related fields
Research InstitutionKyoto University

Principal Investigator

Katayama Takane  京都大学, 生命科学研究科, 教授 (70346104)

Co-Investigator(Kenkyū-buntansha) 松尾 道憲  京都女子大学, 家政学部, 准教授 (00335308)
加藤 紀彦  京都大学, 生命科学研究科, 助教 (40724612)
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords母乳オリゴ糖 / ビフィズス菌 / ラクト-N-テトラオース / シンビオジェニック因子 / 水酸化脂肪酸 / 共生 / クロストーク / 腸内細菌 / 宿主
Outline of Final Research Achievements

Gut microbiota formed during infancy has life-long effects on host health. Breast-fed infant intestines are generally dominated by bifidobacteria, for which oligosaccharides contained in milk play a important role. We have revealed that bifidobacteria possess species/strain-specific pathways for assimilating human milk oligosaccharides among gut microbes, thereby selectively proliferating in the gut ecosystem. In this project, we tried to understand the symbiosis between gut microbes and humans by "symbiogenic factor(s)", chemical compound(s) that supports interplay between the two organisms. As a result, we have identified key genetic factors that contribute to the bifidus flora formation in infant guts. Also, we have developed an efficient enzymatic method to synthesize lacto-N-tetraose, a major core structure of human milk oligosaccharides. These results could serve as scientific basis to improve formula milk.

Academic Significance and Societal Importance of the Research Achievements

申請者はこれまで、乳児腸管におけるビフィズスフローラ形成には母乳オリゴ糖が関与していることを世界に先駆けて明らかとしてきた。本研究では、実際のビフィズスフローラ形成にかかわるビフィズス菌遺伝子を同定すると共に、これまでに単離されたすべてのビフィズス菌が資化可能な母乳オリゴ糖ラクト-N-テトラオースの酵素合成法を開発した。これらの成果は、より人乳に近い調整乳を開発する上で重要な科学的基盤となると考えられる。また、人乳成分を介したビフィズス菌とヒトの共生・共進化を考える上で新しいパラダイムを提供した。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (15 results)

All 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Technical University of Denmark(デンマーク)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Minority species influences microbiota formation: the role of Bifidobacterium with extracellular glycosidases in bifidus flora formation in breastfed infant guts2019

    • Author(s)
      Gotoh Aina、Ojima Miriam Nozomi、Katayama Takane
    • Journal Title

      Microbial Biotechnology

      Volume: 12 Issue: 2 Pages: 259-264

    • DOI

      10.1111/1751-7915.13366

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sharing of human milk oligosaccharides degradants within bifidobacterial communities in faecal cultures supplemented with Bifidobacterium bifidum2018

    • Author(s)
      Gotoh Aina、Katoh Toshihiko、Sakanaka Mikiyasu、Ling Yiwei、Yamada Chihaya、Asakuma Sadaki、Urashima Tadasu、Tomabechi Yusuke、Katayama-Ikegami Ayako、Kurihara Shin、Yamamoto Kenji、Harata Gaku、He Fang、Hirose Junko、Kitaoka Motomitsu、Okuda Shujiro、Katayama Takane
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 13958-13958

    • DOI

      10.1038/s41598-018-32080-3

    • NAID

      120006817605

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Bifidobacterium bifidumはAfcA N末端ドメインを介してムチン糖鎖と相互作用する2018

    • Author(s)
      加藤紀彦・丹沢充裕・桑場潮音・片山高嶺
    • Organizer
      第37会日本糖質学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ABCトランスポーターを介したビフィズス菌の糖質取込みを包括的に制御するATP加水分解酵素2018

    • Author(s)
      阪中幹祥・前田信悟・後藤愛那・村上隆太・加藤紀彦・杉山友太・谷内寛之・栗原新・玉置尚徳・吹谷智・横田篤・片山高嶺
    • Organizer
      2018年日本乳酸菌学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乳児腸管におけるビフィズスフローラ形成機構とその意義2018

    • Author(s)
      片山高嶺
    • Organizer
      ビジョナリー農芸化学100
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 母乳成分を介したビフィズス菌とヒトの共生・共進化2018

    • Author(s)
      片山高嶺
    • Organizer
      酪農科学シンポジウム2018
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 乳児のビフィズス菌とヒトミルクオリゴ糖利用2018

    • Author(s)
      片山高嶺
    • Organizer
      森永乳業創業100周年記念国際シンポジウム
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 母乳成分を介したビフィズス菌とヒトの共生・共進化2018

    • Author(s)
      片山高嶺
    • Organizer
      日本農芸化学会2018年度大会(招待講演)
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] ヒト母乳オリゴ糖とヒト乳脂肪から考えるビフィズス菌とヒトの共進化2017

    • Author(s)
      後藤愛那・岸野重信・岡田奈津実・廣瀬潤子・栗原新・加藤紀彦・奥田修二郎・片山高嶺・小川順
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ABCタンパク質によるコレステロール排出を活性化する腸内細菌由来成分の探索2017

    • Author(s)
      諏訪英理子・池本柚花子・後藤愛那・加藤紀彦・片山 高嶺・松尾道憲
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Book] 腸内フローラの形成と疾患2018

    • Author(s)
      後藤愛那、加藤紀彦、阪中幹祥、片山高嶺
    • Total Pages
      85
    • Publisher
      医薬出版
    • ISBN
      9784990673963
    • Related Report
      2018 Annual Research Report
  • [Remarks]

    • URL

      http://www.bunshioutou.lif.kyoto-u.ac.jp/katayama/Home.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 分子応答機構学分野HP

    • URL

      http://www.bunshioutou.lif.kyoto-u.ac.jp/katayama/Home.html

    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 改変ホスホリラーゼを利用したラクト-N-ビオースIまたはガラクト-N-ビオースのβグリコシドの酵素合成法2018

    • Inventor(s)
      片山高嶺・加藤紀彦・北岡本光
    • Industrial Property Rights Holder
      片山高嶺・加藤紀彦・北岡本光
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-157840
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report

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Published: 2017-07-21   Modified: 2021-01-27  

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