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Development of a non-GM method for crop genome editing

Research Project

Project/Area Number 17K19249
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural chemistry and related fields
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

IMAI Ryozo  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 主席研究員 (90291913)

Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsゲノム編集 / CRISPR / DNAフリー / ジベレリン / 緑の革命 / 短稈 / Ribonucleoprotein / コムギ / RNP / バイオテクノロジー
Outline of Final Research Achievements

we have developed a novel methodology to deliver the CRISPR/Cas9 system directly into germ cells within the Shoot Apical Meristems (SAMs) of wheat. The plants created with the method, which requires no tissue culture steps, inherited mutations to the next generation. We have successfully introduced a mutation in a wheat ortholog of the rice green revolution gene.

Academic Significance and Societal Importance of the Research Achievements

ゲノム編集は新しい作物育種技術として注目されている.しかし,作物のゲノム編集では,一般的に外来遺伝子を染色体に一度組み込み,それを後代の交配等で分離除去する過程を経る.この過程は複雑であり,社会受容の面からは障壁となり得る.そこで,本研究ではDNAを用いないゲノム編集系を作物で開発し、外来遺伝子を染色体に組み込まないゲノム編集技術を開発することを目的とした.本研究で開発された手法により,コムギ実用品種の農業形質を改変することが可能になることが期待される.

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2021-02-19  

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