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Study to elucidate molecular mechanisms involved in orchid-mycorrhiza interaction

Research Project

Project/Area Number 17K19253
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural and Environmental Biology and related fields
Research InstitutionHokkaido University

Principal Investigator

Shimura Hanako  北海道大学, 農学研究院, 講師 (20507230)

Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsラン / 菌根菌 / 種子発芽 / マイコウイルス / ラン科植物 / 共生発芽 / ウイルス / 共生
Outline of Final Research Achievements

Plants in the family Orchidaceae depend on mycorrhizal fungi for seed germination and following seedling development, but little is known about the molecular mechanisms involved in the orchid-fungal interactions. This study aimed to clarify the molecular mechanism involved in the establishment and maintenance of mycorrhizal relationships by using various orchid mycorrhizal fungi with different ability to induce germination. As a factor related to the germination ability, it was suggested that the kind of partitivirus infecting the fungus and the involvement of endornavirus and megabirnavirus. RNAseq analysis using protocorms obtained with or without fungal inoculation was conducted to know the gene expression involved in symbiotic germination, and it was suggested that the defense response-related genes and sugar transport-related genes were involved in regulation of mycorrhizal interaction with fungi.

Academic Significance and Societal Importance of the Research Achievements

植物界で最も多様性があるとされるラン科植物がもつ特殊な共生関係はランの生存に必須であり、進化の過程でも失われずに維持されてきた重要なメカニズムである。しかし、その制御に関わる要因については研究が進んでいない。本研究では、共生関係を制御するメカニズムの一因として、菌根菌に感染するウイルスや共生特異的に発現する遺伝子を示唆することができた。さらなる解明により、植物と菌の相互作用に及ぼす内在ウイルスの存在意義を明らかにし、ランと菌根菌の進化学に新たな研究領域を開拓することも期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2020 2018

All Journal Article (1 results) Presentation (3 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Metagenomic analyses of the viruses detected in mycorrhizal fungi and their host orchid.2018

    • Author(s)
      Shimura H., Masuta C., Koda Y.
    • Journal Title

      Methods in Molecular Biology,

      Volume: 1746 Pages: 161-172

    • DOI

      10.1007/978-1-4939-7683-6_12

    • ISBN
      9781493976829, 9781493976836
    • Related Report
      2018 Research-status Report
  • [Presentation] RNAseqによるランの共生発芽に関わる分子メカニズム解明に向けた遺伝子の探索2020

    • Author(s)
      稲島七海・平田智恵子・藤野介延・志村華子
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ラン-菌根菌共生系に影響を及ぼすウイルス探索の試み2018

    • Author(s)
      志村華子・増田税・幸田泰則
    • Organizer
      平成30年度(第53回)植物感染生理談話会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] ラン-菌根菌共生系に影響を及ぼすウイルス探索の試み2018

    • Author(s)
      志村華子
    • Organizer
      平成30年度(第53回)植物感染生理談話会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] Metagenomic Analyses of the Viruses Detected in Mycorrhizal Fungi and Their Host Orchid. In: Pantaleo V, Chiumenti. (eds) Viral Metagenomics. Methods in Molecular Biology, vol 1746.2018

    • Author(s)
      Shimura H, Masuta C, Koda Y
    • Total Pages
      217
    • Publisher
      Humana Press, New York
    • ISBN
      9781493976829
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2021-02-19  

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