• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Discovery and dissolution of the constraints that have been derived to plants during their adaptation process to nature

Research Project

Project/Area Number 17K19257
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural and Environmental Biology and related fields
Research InstitutionTohoku University

Principal Investigator

Miyashita Shuhei  東北大学, 農学研究科, 助教 (60556710)

Research Collaborator Suzuki Machi  
Sietske van Bentum  
Saijo Yuki  
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords進化 / 栽培化 / 数理モデリング / ウイルス / 植物 / 抵抗性 / R遺伝子 / MOI
Outline of Final Research Achievements

Plant genomes contain many R genes, of which products function as receptors to detect pathogens to induce resistance against them. In many cases, R-gene mediated resistance cause autonomous cell death, which might contribute for inclusion of the pathogens to the infected regions. The current study showed that MOI, the average number of viral genomes that establish infection in a new cell, decreases in the presence of an R gene, even before the occurrence of cell death. A simulation model was developed to show that systemic necrosis, which occurs upon insufficient induction of resistance, can function as a suicide strategy against pathogens, by helping the infected individuals avoid becoming an infection source to neighbor plants, which are often kins in the case of plants.

Academic Significance and Societal Importance of the Research Achievements

全ての植物に備わっているR遺伝子によるウイルス抵抗性を、数字(MOI)で記述できる可能性を本研究で示すことができた。これにより例えば、ウイルス抵抗性機構へのそれぞれの遺伝子の寄与を数字で評価できるようになる可能性がある。このことは、新しいウイルス抵抗性作物の作出に寄与する可能性がある。また、不完全な抵抗性誘導であっても自然界においては集団レベルでの生存戦略として機能する可能性を本研究で示すことができた。このことは、R遺伝子の進化過程の学術的理解に大きく寄与するものであるとともに、応用研究においては新奇R遺伝子の探索に新たな可能性を与えるものであると考えられる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (30 results)

All 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (19 results) (of which Int'l Joint Research: 5 results,  Invited: 10 results) Remarks (2 results)

  • [Int'l Joint Research] ワーゲニンゲン大学/ユトレヒト大学(オランダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] ワーゲニンゲン大学/ユトレヒト大学(オランダ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] First identification and characterization of cucumber mosaic virus from Corchorus olitorius in Japan2018

    • Author(s)
      Hanbal Sara E.、Miyashita Shuhei、Ando Sugihiro、Sidaros Samir A.、Takahashi Hideki
    • Journal Title

      Journal of Plant Pathology

      Volume: 100 Issue: 3 Pages: 561-565

    • DOI

      10.1007/s42161-018-0099-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Atmospheric-pressure plasma irradiation can disrupt tobacco mosaic virus particles and RNAs to inactivate their infectivity2018

    • Author(s)
      Hanbal Sara E.、Takashima Keisuke、Miyashita Shuhei、Ando Sugihiro、Ito Kumiko、Elsharkawy Mohsen M.、Kaneko Toshiro、Takahashi Hideki
    • Journal Title

      Archives of Virology

      Volume: 163 Issue: 10 Pages: 2835-2840

    • DOI

      10.1007/s00705-018-3909-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Survey of the response of 82 domestic landraces of Zea mays to cucumber mosaic virus reveals geographical region-related resistance to CMV in Japan2018

    • Author(s)
      Takahashi H.、Tian A.、Miyashita S.、Kanayama Y.、Ando S.、Kormelink R.
    • Journal Title

      Plant Pathology

      Volume: in press Issue: 6 Pages: 1401-1415

    • DOI

      10.1111/ppa.12848

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Studies on replication and evolution mechanisms of plant RNA viruses2018

    • Author(s)
      MIYASHITA S.
    • Journal Title

      Japanese Journal of Phytopathology

      Volume: 84 Issue: 3 Pages: 149

    • DOI

      10.3186/jjphytopath.84.149

    • NAID

      130007472317

    • ISSN
      0031-9473, 1882-0484
    • Related Report
      2018 Annual Research Report
  • [Journal Article] Studies on replication and evolution mechanisms of plant RNA viruses2018

    • Author(s)
      Miyashita Shuhei
    • Journal Title

      Journal of General Plant Pathology

      Volume: 84 Issue: 6 Pages: 427-428

    • DOI

      10.1007/s10327-018-0806-3

    • NAID

      130007472317

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Comparative analysis of microbial diversity and bacterial seedling disease-suppressive activity in organic-farmed and standardized commercial conventional soils for rice nursery cultivation2018

    • Author(s)
      Takahashi Hideki、Matsushita Yuko、Ito Toyoaki、Nakai Yutaka、Nanzyo Masami、Kobayashi Takashi、Iwaishi Shinji、Hashimoto Tomoyoshi、Miyashita Shuhei、Morikawa Toshiyuki、Yoshida Shigenobu、Tsushima Seiya、Ando Sugihiro
    • Journal Title

      Journal of Phytopathology

      Volume: 166 Issue: 4 Pages: 249-264

    • DOI

      10.1111/jph.12682

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased cytosine methylation at promoter of the NB-LRR class R gene RCY1 correlated with compromised resistance to cucumber mosaic virus in EMS-generated src mutants of Arabidopsis thaliana2017

    • Author(s)
      Sato Yukiyo、Miyashita Shuhei、Ando Sugihiro、Takahashi Hideki
    • Journal Title

      Physiol Mol Plant Pathol

      Volume: 100 Pages: 151-162

    • DOI

      10.1016/j.pmpp.2017.09.007

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 植物ウイルス-植物個体群相互作用における空間構造の効果のシミュレーションによる検討2019

    • Author(s)
      宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成31年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物病原性Verticillium属菌から検出された複数のRNAウイルスのゲノム全塩基配列決定2019

    • Author(s)
      宮下脩平・Michael F Seidl・Jasper RL Depotter・安藤杉尋・高橋英樹・Bart PHJ Thomma
    • Organizer
      第72回北日本病害虫研究発表会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A simple evolution model of MOI unveils the distinct biological principles behind different classes of viruses.2019

    • Author(s)
      Shuhei Miyashita
    • Organizer
      International Neovirology Mini-symposium Series IV
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Social aspects in viral ways of life, analyzed by wet experiments and dry simulations.2018

    • Author(s)
      Shuhei Miyashita
    • Organizer
      WUR-TU Plant Science Workshop 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物病原性Verticillium属菌から検出された複数のRNAウイルスのゲノム全塩基配列決定2018

    • Author(s)
      宮下脩平・Michael F Seidl・Jasper RL Depotter・安藤杉尋・高橋英樹・Bart PHJ Thomma
    • Organizer
      平成30年度日本植物病理学会東北部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 植物病原性 Verticillium属菌の RNA ウイルス2018

    • Author(s)
      宮下脩平
    • Organizer
      ネオウイルス学ミニシンポジウム 1
    • Related Report
      2018 Annual Research Report
  • [Presentation] Evolution of strategies in space: the never-ending quests for higher fitness by viruses and the hosts driven by differences and changes in spatial structures.2018

    • Author(s)
      Shuhei Miyashita
    • Organizer
      The 17th Awaji International Forum on Infection and Immunity
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Majority decisions by four or five members: social rules that can be formed and maintained in viral populations to exclude free riders.2018

    • Author(s)
      Shuhei Miyashita
    • Organizer
      Seminaire BGPI CIRAD
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Majority decisions by four or five members: rules that can be formed and maintained in viral populations to exclude free riders.2018

    • Author(s)
      Shuhei Miyashita, Akira Sasaki, Machi Suzuki, Sietske van Bentum,Sugihiro Ando, Masayuki Ishikawa, and Hideki Takahashi
    • Organizer
      2018 Annual Meeting of the Society for Mathematical Biology & the Japanese Society for Mathematical Biology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞間移行におけるCMVのMOIはR遺伝子による抵抗性誘導時に低下する2018

    • Author(s)
      鈴木万智・Sietske van Bentum・安藤杉尋・高橋英樹・宮下脩平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Presentation] R遺伝子による認識を免れる変異型CMV CPのアラニンスキャニングによる探索2018

    • Author(s)
      西條悠希・安藤杉尋・高橋英樹・宮下脩平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
  • [Presentation] 植物RNAウイルスの 複製と進化機構に関する研究2018

    • Author(s)
      宮下脩平
    • Organizer
      平成30年度日本植物病理学会大会
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Invited
  • [Presentation] 植物ウイルスの社会システムを標的とする防除技術の開発2017

    • Author(s)
      宮下脩平
    • Organizer
      第2回えこえぴワークショップ
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] RNAウイルス欠失変異の網羅的検出系の確立2017

    • Author(s)
      宮下脩平・本多宗一郎・安藤杉尋・高橋英樹
    • Organizer
      平成29年度植物感染生理談話会
    • Related Report
      2017 Research-status Report
  • [Presentation] ウイルス社会学―ウイルスの社会システムを撹乱せよ!2017

    • Author(s)
      宮下脩平
    • Organizer
      第16回みちのくウイルス塾
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Social systems of plant viruses revealed by molecular biology experiments and mathematical modeling2017

    • Author(s)
      Shuhei Miyashita
    • Organizer
      Tohoku Forum for Creativity Fusion Research Seminar
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 植物ウイルスのもつ社会システムを、実験と数理モデリングで覗き見る2017

    • Author(s)
      宮下脩平
    • Organizer
      ウイルス研究の潮流シリーズセミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] キュウリモザイクウイルスの欠失変異パターンの解析2017

    • Author(s)
      本多宗一郎・安藤杉尋・高橋英樹・宮下脩平
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 植物ウイルスの多数決型意思決定システム2017

    • Author(s)
      宮下脩平・安藤杉尋・高橋英樹
    • Organizer
      平成29年度日本植物病理学会大会
    • Related Report
      2017 Research-status Report
  • [Remarks] 東北大学大学院農学研究科 植物病理学分野

    • URL

      http://www.agri.tohoku.ac.jp/ppathol/index.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 東北大学大学院農学研究科 お知らせ/ニュース

    • URL

      http://www.agri.tohoku.ac.jp/jp/news/news/detail/20180406-2.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi