Regulation of meiotic recombination via sequence-specific nucleases
| Project/Area Number |
17K19277
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Agricultural and Environmental Biology and related fields
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
Saika Hiroaki 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (20435613)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 育種学 / バイオテクノロジー / 遺伝学 / ゲノム |
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| Outline of Final Research Achievements |
In this study, I attempted to develop the technology for the regulation of the recombination spots mediated by CRISPR/Cas9 in meiosis. First, transgenic plants were produced as a material for monitoring meiotic recombination events visually in rice and Arabidopsis. Besides, to express CRISPR/Cas9 during meiosis, 3-kb of upstream regions in 3 genes that strongly express in meiosis were isolated. Transformed rice harboring CRISPR/Cas9 driven by these promoters were produced. However, there were no targeted mutagenesis events in these plants in the T1 generation.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究を発展させることで、交配育種による新品種作出をより簡便に低労力で行うことが可能になる。
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Report
(4 results)
Research Products
(6 results)
