Regulation of meiotic recombination via sequence-specific nucleases

• Project/Area Number: 17K19277
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Agricultural and Environmental Biology and related fields
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Saika Hiroaki 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (20435613)
• Project Period (FY): 2017-06-30 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 育種学 / バイオテクノロジー / 遺伝学 / ゲノム

Outline of Final Research Achievements

In this study, I attempted to develop the technology for the regulation of the recombination spots mediated by CRISPR/Cas9 in meiosis. First, transgenic plants were produced as a material for monitoring meiotic recombination events visually in rice and Arabidopsis. Besides, to express CRISPR/Cas9 during meiosis, 3-kb of upstream regions in 3 genes that strongly express in meiosis were isolated. Transformed rice harboring CRISPR/Cas9 driven by these promoters were produced. However, there were no targeted mutagenesis events in these plants in the T1 generation.

Academic Significance and Societal Importance of the Research Achievements

本研究を発展させることで、交配育種による新品種作出をより簡便に低労力で行うことが可能になる。

Research Products

• [Journal Article] 精密ゲノム編集の現状と展望 (2019)
  • Author(s): 雑賀啓明、土岐精一
  • Journal Title: 生物工学 Volume: 97 Pages: 728-731
  • Peer Reviewed / Open Access
• [Journal Article] イネのゲノム編集―その動向と展望― (2017)
  • Author(s): 雑賀啓明、土岐精一
  • Journal Title: 化学と生物 Volume: 55 Pages: 676-683
  • Peer Reviewed
• [Presentation] Precise mutagenesis and its application in rice (2019)
  • Author(s): Hiroaki Saika, Akiko Mori, Seiichi Toki
  • Organizer: 2nd International Conference "Plant Genome Editing & Genome Engineering"
  • Int'l Joint Research / Invited
• [Presentation] イネの精密ゲノム編集 (2019)
  • Author(s): 雑賀啓明、根岸克弥、廣瀬咲子、横井彩子、遠藤真咲、土岐精一
  • Organizer: イネ遺伝学・分子生物学ワークショップ2019
• [Presentation] Precise genome editing in rice (2019)
  • Author(s): Hiroaki Saika, Katsuya Negishi, Sakiko Hirose, Ayako Nishizawa-Yokoi, Masaki Endo, Seiichi Toki
  • Organizer: 2019 KRIBB Conference
  • Int'l Joint Research / Invited
• [Presentation] Targeted deletion of rice retrotransposon (2019)
  • Author(s): Hiroaki Saika, Mori Akiko, Masaki Endo, Seiichi Toki
  • Organizer: Frontiers in Genome Engineering 2019
  • Int'l Joint Research