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Research on microbial loop unique to lake hypolimnion

Research Project

Project/Area Number 17K19289
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Forestry and Forest Products Science, Applied aquatic science, and related fields
Research InstitutionKyoto University

Principal Investigator

Nakano Shin-ichi  京都大学, 生態学研究センター, 教授 (50270723)

Research Collaborator Hodoki Yoshikuni  京都大学, 生態学研究センター, 特定准教授 (60632122)
Okazaki Yusuke  産業技術総合研究所, 生物プロセス研究部門 生物資源情報基盤研究グループ, 日本学術振興会 特別研究員 (40823745)
Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords湖沼 / 深水層 / 微生物ループ / CL500-11細菌 / キネトプラスチド鞭毛虫 / ピコ植物プランクトン / 微生物群集組成 / 細菌 / 原生生物 / 鞭毛虫 / 湖沼深水層 / 群集ゲノム解析 / 食物連鎖
Outline of Final Research Achievements

The aim of the present study was to elucidate the food linkage between bacteria and protists, so-called “Microbial loop”, in hypolimnion of lakes. In the hypolimnion of Lake Biwa, the dominant bacterial lineages were Bacteroidetes, Actinobacteria, Chloroflexi and Planctomycetes. Shotgun metagenomic analyses suggested the physiological and ecological characteristics of dominant bacterial species. It was likely that kinetoplastid flagellates were the most important grazers on bacteria. In the hypolimnion of Lakes Ikeda and Tazawa, kinetoplastid and cercozoa flagellates were suggested as important grazers on bacteria.

Academic Significance and Societal Importance of the Research Achievements

現在、「湖沼を地域の資源として活用していく」という考え方がある。この考え方では、「地域の望ましい湖沼像(地域の湖沼のあるべき姿)」を当該湖沼のステークホルダー(行政、地域住民、民間団体、事業者など)で共同して設定・共有し、地域で湖沼の管理方法を決める。これを行う際、地域の資源として湖沼がどのような姿をもっているか、特に地域特有の生物資源の存否は重要な観点となる。本研究では、各湖沼に特有な細菌種の生息が確認され、またそれらの細菌種のいくつかについては原生生物・鞭毛虫を介した食物連鎖も明らかとなった。これらの情報は、各湖沼特有の生物資源や生態系特性の発見として今後の湖沼管理に有益な情報となる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (12 results)

All 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] Biology Center, Czech Academy of Science(チェコ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 水生生物学研究所、生物学センター(チェコ)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Hidden in plain sight?highly abundant and diverse planktonic freshwater Chloroflexi2018

    • Author(s)
      Mehrshad Maliheh、Salcher Michaela M、Okazaki Yusuke、Nakano Shin-ichi、?imek Karel、Andrei Adrian-Stefan、Ghai Rohit
    • Journal Title

      Microbiome

      Volume: 6 Issue: 1 Pages: 176-176

    • DOI

      10.1186/s40168-018-0563-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Long-term Changes in Water Quality of Lake Biwa with Special Reference to Organic Matter Dynamics and Microbial Ecology2018

    • Author(s)
      Shin-ichi Nakano, Kazuhide Hayakawa, Yoshikuni Hodoki, Yusuke Okazaki, Indranil Mukherjee, Shoji D. Thottathil, Hiroyuki Takasu, Shohei Fujinaga
    • Journal Title

      Proceedings of the 17th World Lake Conference

      Volume: 1 Pages: 304-306

    • Related Report
      2018 Annual Research Report
  • [Journal Article] The Broad Habitat Spectrum of the CL500-11 Lineage (Phylum Chloroflexi), a Dominant Bacterioplankton in Oxygenated Hypolimnia of Deep Freshwater Lakes2018

    • Author(s)
      Okazaki Yusuke、Salcher Michaela M.、Callieri Cristiana、Nakano Shin-ichi
    • Journal Title

      Frontiers in Microbiology

      Volume: 9 Pages: 2891-2891

    • DOI

      10.3389/fmicb.2018.02891

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Long-term changes in water quality in Lake Biwa with special reference to organic matter dynamics, microbial ecology and diversity2017

    • Author(s)
      Nakano S, K Hayakawa, Y Hodoki, Y Okazaki, I Mukherjee, SD Thottathil, H Takasu, S Fujinaga
    • Journal Title

      Proceedings of the second International Conference on Life Science and Biotechnology (ICOLIB)

      Volume: 2

    • Related Report
      2017 Research-status Report
  • [Presentation] Long-term Changes in Water Quality of Lake Biwa with Special Reference to Organic Matter Dynamics and Microbial Ecology2018

    • Author(s)
      Shin-ichi Nakano, Kazuhide Hayakawa, Yoshikuni Hodoki, Yusuke Okazaki, Indranil Mukherjee, Shoji D. Thottathil, Hiroyuki Takasu, Shohei Fujinaga
    • Organizer
      Proceedings of the 17th World Lake Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 琵琶湖の底にある、ちょっと変わった微生物ループ2018

    • Author(s)
      中野伸一、早川和秀、程木義邦、岡崎友輔、I. Mukherjee、S. D. Thottathil、 高巣裕之
    • Organizer
      日本生態学会第65回札幌大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メタゲノム解析で明らかにする湖の微生物生態系2018

    • Author(s)
      岡崎友輔
    • Organizer
      第83回日本陸水学会岡山大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Long-term changes in water quality in Lake Biwa with special reference to organic matter dynamics, microbial ecology and diversity2017

    • Author(s)
      Nakano S, K Hayakawa, Y Hodoki, Y Okazaki, I Mukherjee, SD Thottathil, H Takasu, S Fujinaga
    • Organizer
      The second International Conference on Life Science and Biotechnology (ICOLIB)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Seasonal variations of autotrophic picoplankton abundance in Lake Biwa, with special reference to their grazers2017

    • Author(s)
      蔡吉、程木義邦、中野伸一
    • Organizer
      日本陸水学会第82回田沢湖大会
    • Related Report
      2017 Research-status Report
  • [Remarks] 二湖沼におけるユニークな手法の組み合わせによる鞭毛虫と細菌の群集動態と相互作用

    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2020-03-30  

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