• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Does oxygen supplied to anaerobic soil by mangrove aerial roots facilitate soil nitrate production and plant nitrate use?

Research Project

Project/Area Number 17K19292
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Forestry and Forest Products Science, Applied aquatic science, and related fields
Research InstitutionKyoto University

Principal Investigator

Koyama Lina  京都大学, 情報学研究科, 准教授 (50378832)

Co-Investigator(Kenkyū-buntansha) 井上 智美  国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, 主任研究員 (80435578)
松尾 奈緒子  三重大学, 生物資源学研究科, 講師 (00423012)
大手 信人  京都大学, 情報学研究科, 教授 (10233199)
黒岩 恵  東京農工大学, 工学(系)研究科(研究院), 助教 (00761024)
Project Period (FY) 2017-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsマングローブ / 硝酸態窒素 / オヒルギ / ヤエヤマヒルギ / 硝化 / 硝酸還元酵素 / 根圏 / アンモニア酸化アーキア / アンモニア酸化細菌 / マングローブ植物 / 嫌気性土壌 / 窒素循環 / 土壌微生物
Outline of Final Research Achievements

This study aimed to investigate dynamics of nitrogen, an essential macronutrient, in mangrove ecosystems from a novel viewpoint. Mangrove forests are regularly exposed to tides and the soil frequently becomes hypoxic. Soil inorganic nitrogen, ammonium and nitrate, are the major nitrogen sources for plants, and nitrate production (nitrification) does not occur in hypoxic soil condition. Thus, nitrate has not been considered as a major nitrogen source for mangrove forests. In this study, we tested the hypothesis that plants supply oxygen via the roots to the soil, and nitrifier microbes produce nitrate using the oxygen. The results showed that nitrification occurred in mangrove soil, and that mangrove species have high capacities to use nitrate as a nitrogen source.

Academic Significance and Societal Importance of the Research Achievements

本研究は、マングローブ林生態系の維持機構について、土壌微生物とマングローブ植物の酸素に関する共生的関係に着目した。学術的意義として、これまでマングローブ植物の窒素源としてはほとんど考慮されてこなかった硝酸態窒素に着目し、マングローブ土壌中の硝酸生成について把握を行った。加えて、マングローブ植物は硝酸態窒素を窒素源として利用する能力を持ち、硝酸態窒素に対する親和性の高いタイプの種が存在することを示した植物生理生態学的意義が挙げられる。社会的意義として、これまで着目されてこなかった植物-微生物間の共生的関係を示したことにより、マングローブ林の成立・維持機構について新しい知見をもたらした。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (46 results)

All 2021 2020 2019 2018

All Journal Article (21 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 19 results,  Open Access: 9 results) Presentation (21 results) (of which Int'l Joint Research: 5 results,  Invited: 4 results) Book (4 results)

  • [Journal Article] Theoretical analysis of temperature‐dependent model of respiratory O2 consumption using the kinetics of the cytochrome and alternative pathways.2021

    • Author(s)
      Inoue T, Noguchi K
    • Journal Title

      New Phytologist

      Volume: 229 Issue: 3 Pages: 1810-1821

    • DOI

      10.1111/nph.16964

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Respective contribution of urban wastewater and mangroves on nutrient dynamics in a tropical estuary during the monsoon season2020

    • Author(s)
      Taillardat Pierre、Marchand Cyril、Friess Daniel A.、Widory David、David Frank、Ohte Nobuhito、Nakamura Takashi、Van Vinh Truong、Thanh-Nho Nguyen、Ziegler Alan D.
    • Journal Title

      Marine Pollution Bulletin

      Volume: 160 Pages: 111652-111652

    • DOI

      10.1016/j.marpolbul.2020.111652

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Dissimilatory Nitrate Reduction to Ammonium and Responsible Microbes in Japanese Rice Paddy Soil2020

    • Author(s)
      Nojiri Yosuke、Kaneko Yuka、Azegami Yoichi、Shiratori Yutaka、Ohte Nobuhito、Senoo Keishi、Otsuka Shigeto、Isobe Kazuo
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 4 Pages: n/a

    • DOI

      10.1264/jsme2.ME20069

    • NAID

      130007923243

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Annual Research Report
  • [Journal Article] Distinct Community Composition of Previously Uncharacterized Denitrifying Bacteria and Fungi across Different Land-Use Types2020

    • Author(s)
      Fujimura Reiko、Azegami Yoichi、Wei Wei、Kakuta Hiroko、Shiratori Yutaka、Ohte Nobuhito、Senoo Keishi、Otsuka Shigeto、Isobe Kazuo
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME19064

    • NAID

      130007792089

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] COST ANALYSIS OF MULTIPLE PROTECTION MEASURES INCORPORATING GREEN INFRASTRUCTURE IN COASTAL AREAS2020

    • Author(s)
      熊野直子、田村誠、井上智美、横木裕宗
    • Journal Title

      Journal of Japan Society of Civil Engineers, Ser. G (Environmental Research)

      Volume: 76 Issue: 5 Pages: I_221-I_231

    • DOI

      10.2208/jscejer.76.5_I_221

    • NAID

      130007970548

    • ISSN
      2185-6648
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O2020

    • Author(s)
      Kuroiwa Megumi、Fukushima Keitaro、Hashimoto Kazuma、Senga Yukiko、Sato Tsubasa、Katsuyama Chie、Suwa Yuichi
    • Journal Title

      Journal of Visualized Experiments

      Volume: - Issue: 164

    • DOI

      10.3791/59562

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The physiological potential of anammox bacteria as revealed by their core genome structure2020

    • Author(s)
      Okubo Takashi、Toyoda Atsushi、Fukuhara Kohei、Uchiyama Ikuo、Harigaya Yuhki、Kuroiwa Megumi、Suzuki Takuma、Murakami Yuka、Suwa Yuichi、Takami Hideto
    • Journal Title

      DNA Research

      Volume: 28 Issue: 1 Pages: 1-12

    • DOI

      10.1093/dnares/dsaa028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mangrove development and carbon storage on an isolated coral atoll2020

    • Author(s)
      Yamano Hiroya、Inoue Tomomi、Baba Shigeyuki
    • Journal Title

      Environmental Research Communications

      Volume: 2 Issue: 6 Pages: 065002-065002

    • DOI

      10.1088/2515-7620/ab9d05

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrate reductase activities in plants from different ecological and taxonomic groups grown in Japan2020

    • Author(s)
      Koyama Lina A.、Terai Masakazu、Tokuchi Naoko
    • Journal Title

      Ecological Research

      Volume: - Issue: 5 Pages: 708-712

    • DOI

      10.1111/1440-1703.12083

    • NAID

      120006888515

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Consequences of microbial diversity in forest nitrogen cycling: diverse ammonifiers and specialized ammonia oxidizers2020

    • Author(s)
      Kazuo Isobe, Yuta Ise, Hiroyu Kato, Tomoki Oda, Christian E Vincenot, Keisuke Koba, Ryunosuke Tateno, Keishi Senoo, Nobuhito Ohte
    • Journal Title

      The ISME journal

      Volume: 14 Issue: 1 Pages: 12-25

    • DOI

      10.1038/s41396-019-0500-2

    • NAID

      120006800755

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tracking the route of atmospheric nitrogen to diazotrophs colonizing buried mangrove roots2019

    • Author(s)
      Inoue Tomomi、Kohzu Ayato、Shimono Ayako
    • Journal Title

      Tree Physiology

      Volume: 39 Issue: 11 Pages: 1896-1906

    • DOI

      10.1093/treephys/tpz088

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mangrove - diazotroph relationships at the root, tree and forest scales: diazotrophic communities create high soil nitrogenase activities in Rhizophora stylosa rhizospheres2019

    • Author(s)
      Inoue Tomomi、Shimono Ayako、Akaji Yasuaki、Baba Shigeyuki、Takenaka Akio、Tuck Chan Hung
    • Journal Title

      Annals of Botany

      Volume: 125 Pages: 131-144

    • DOI

      10.1093/aob/mcz164

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of nitrate export patterns in forested catchments in Japan and the Northeastern United States: A meta‐analysis2019

    • Author(s)
      Ohte Nobuhito、Katsuyama Masanori
    • Journal Title

      Hydrological Processes

      Volume: 33 Issue: 25 Pages: 3184-3194

    • DOI

      10.1002/hyp.13612

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Assessing nutrient dynamics in mangrove porewater and adjacent tidal creek using nitrate dual-stable isotopes: A new approach to challenge the Outwelling Hypothesis?2019

    • Author(s)
      Taillardat Pierre、Ziegler Alan D.、Friess Daniel A.、Widory David、David Frank、Ohte Nobuhito、Nakamura Takashi、Evaristo Jaivime、Thanh-Nho Nguyen、Van Vinh Truong、Marchand Cyril
    • Journal Title

      Marine Chemistry

      Volume: 214 Pages: 103662-103662

    • DOI

      10.1016/j.marchem.2019.103662

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Holocene sea-level change and evolution of a mixed coral reef and mangrove system at Iriomote Island, southwest Japan2019

    • Author(s)
      Yamano Hiroya、Inoue Tomomi、Adachi Hiroshi、Tsukaya Keisuke、Adachi Ryota、Baba Shigeyuki
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 220 Pages: 166-175

    • DOI

      10.1016/j.ecss.2019.03.001

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Photosynthesis, respiration, and growth patterns of Rhizophora stylosa seedlings in relation to growth temperature2019

    • Author(s)
      Akaji Y, Inoue T, Tomimatsu H, Kawanishi A
    • Journal Title

      Trees

      Volume: 未定 Issue: 4 Pages: 1041-1049

    • DOI

      10.1007/s00468-019-01840-7

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of cryogenic vacuum distillation on the stable isotope ratios of soil water2019

    • Author(s)
      Tsuruta Kenji、Yamamoto Hiroyuki、Katsuyama Masanori、Kosugi Yoshiko、Okumura Motonori、Matsuo Naoko
    • Journal Title

      Hydrological Research Letters

      Volume: 13 Issue: 1 Pages: 1-6

    • DOI

      10.3178/hrl.13.1

    • NAID

      130007586372

    • ISSN
      1882-3416
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anammox and denitrification in the intertidal sediment of the hypereutrophic Yatsu tidal flat, Japan.2019

    • Author(s)
      Senga Y, Sato T, Kuroiwa M, Nohara S, Suwa Y
    • Journal Title

      Estuaries and Coasts

      Volume: 42 Issue: 3 Pages: 665-674

    • DOI

      10.1007/s12237-019-00520-6

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stream Runoff and Nitrate Recovery Times After Forest Disturbance in the USA and Japan2018

    • Author(s)
      Oda Tomoki、Green Mark B.、Urakawa Rieko、Scanlon Todd M.、Sebestyen Stephen D.、McGuire Kevin J.、Katsuyama Masanori、Fukuzawa Karibu、Adams Mary B.、Ohte Nobuhito
    • Journal Title

      Water Resources Research

      Volume: 54 Issue: 9 Pages: 6042-6054

    • DOI

      10.1029/2017wr021986

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Black spruce assimilates nitrate in boreal winter2018

    • Author(s)
      Koyama Lina A、Kielland Knut
    • Journal Title

      Tree Physiology

      Volume: in press Issue: 4 Pages: 536-543

    • DOI

      10.1093/treephys/tpy109

    • NAID

      120006636644

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nitrate is an important nitrogen source for Arctic tundra plants2018

    • Author(s)
      Liu Xue-Yan、Koba Keisuke、Koyama Lina A.、Hobbie Sarah E.、Weiss Marissa S.、Inagaki Yoshiyuki、Shaver Gaius R.、Giblin Anne E.、Hobara Satoru、Nadelhoffer Knute J.、Sommerkorn Martin、Rastetter Edward B.、Kling George W.、Laundre James A.、Yano Yuriko、Makabe Akiko、Yano Midori、Liu Cong-Qiang
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 115 Issue: 13 Pages: 3398-3403

    • DOI

      10.1073/pnas.1715382115

    • NAID

      120006416235

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マングローブ植物が土壌の窒素養分条件に及ぼす影響2021

    • Author(s)
      森岡たまき, 小山里奈, 井上智美, 黒岩恵
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マングローブ植物呼吸根の通気組織を介したガス輸送は低窒素環境への適応なのか?2021

    • Author(s)
      井上智美, 高津文人, 下野綾子
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] マングローブ植物の窒素源としての硝酸態窒素2021

    • Author(s)
      小山里奈, 井上智美, 松尾奈緒子
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] ニッチが異なるヒルギ科二種の菌根共生と細根形態様式2021

    • Author(s)
      赤路康朗, 井上智美, 谷口武士, 馬場繁幸
    • Organizer
      日本生態学会第68回全国大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Mapping changes in indigenous pastoral ecology in West India: Impact evaluation of climate change and socio-economic changes using participatory mapping and geographical information system2020

    • Author(s)
      Nobuhito Ohte, Ranit Chatterjee, Kai Yamamoto, Pankaj Joshi, Shilpi Shrivastava, Lyla Mehta
    • Organizer
      AGU Fall Meeting 2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Spatial variation in soil physical properties and effects on soil NO3窶? production on forest hillslopes2020

    • Author(s)
      Tomoki Oda, Megumi Kuroiwa, Naoya Fujime, Kazuo Isobe, Naoya Masaoka, Kazumichi Fujii, Hiroto Toda, and Nobuhito Ohte
    • Organizer
      EGU General Assembly 2020
    • Related Report
      2020 Annual Research Report
  • [Presentation] Capacity of two mangrove species, Bruguiera gymnorhiza and Rhizophora stylosa, to use nitrate as a N source2020

    • Author(s)
      Lina A. KOYAMA, Tomomi INOUE, Naoko MATSUO
    • Organizer
      OrgN 2020
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 低酸素状態のマングローブ根圏で硝化は起きているか?―植物と土壌微生物の相互作用2020

    • Author(s)
      森岡たまき, 小山里奈, 黒岩 恵, 井上智美, 松尾奈緒子, 大手信人
    • Organizer
      日本生態学会第67回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Measuring spatial variability of soil N at small scales -A pilot study using microdialysis in boreal forests2020

    • Author(s)
      Lina A. KOYAMA, Scott BUCKLEY, Torgny NASHOLM, Sandra JAMTGARD
    • Organizer
      日本生態学会第67回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] マングローブ植物の葉と根の呼吸速度温度依存性2020

    • Author(s)
      井上智美, 赤路康朗, 野口航
    • Organizer
      日本生態学会第67回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] ヒルギ科二種の根および根近傍土壌に存在するアーバスキュラー菌根菌群集構造2020

    • Author(s)
      赤路康朗, 井上智美, 谷口武士, 馬場繁幸
    • Organizer
      日本生態学会第67回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] マングローブ林形成に伴う根圏窒素固定細菌群集の変化2020

    • Author(s)
      下野 綾子, 井上 智美
    • Organizer
      日本生態学会第67回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Kinetic analysis of the nitrogen metabolisms in the anammox reactor2020

    • Author(s)
      Megumi KUROIWA, Takuma SUZUKI, Hideto TAKAMI, Takashi OKUBO, Yuichi SUWA
    • Organizer
      日本生態学会第67回全国大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Implications of the difference in seasonal variations of nitrate discharge from the temperate forest ecosystems between monsoon and non-monsoon zones2019

    • Author(s)
      Nobuhito Ohte, Masanori Katsuyama
    • Organizer
      AGU Fall Meeting 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 日中および夜間の生育温度上昇に対するヤエヤマヒルギ実生の形態的・生理的変化2019

    • Author(s)
      赤路康朗、井上智美
    • Organizer
      日本生態学会第66回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] マングローブ植物への新たな窒素供給経路の検証―呼吸根通気システムと窒素固定菌2019

    • Author(s)
      井上智美、高津文人、下野綾子
    • Organizer
      日本生態学会第66回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Nitrogen biogeochemistry in catchment ecosystems in Japan: Uniqueness and its imprecations2019

    • Author(s)
      N. Ohte
    • Organizer
      Special Lecture at Department of Geography and Environmental Development, Ben-Gurion University of the Negev, Be’er Sheva, Israel
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Geographical comparison leads to a deeper understanding on the nitrate export mechanisms in forest catchments: To generalizations from unique cases2019

    • Author(s)
      N. Ohte and M. Katsuyama
    • Organizer
      日本生態学会第66回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] The Estimation of CO2 Flux in Subtropical Coastal Ecosystems Using a Numerical Model2018

    • Author(s)
      Moki H., Sohma A., Shibuki H., Toyoda K., Akhand A., Watanabe K., Tokoro T., Inoue T., Yamano H., Banno M., Nakagawa Y., Matsuda H., Kuwae T.
    • Organizer
      JpGU-AGU Joint Meeting 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 生育温度がヤエヤマヒルギ実生の生残・成長・光合成に与える影響2018

    • Author(s)
      赤路康朗、井上智美、冨松元、川西あゆみ
    • Organizer
      中国四国地区生物系三学会合同大会
    • Related Report
      2018 Research-status Report
  • [Presentation] マングローブ根圏に形成される窒素固定細菌群集2018

    • Author(s)
      下野綾子、井上智美
    • Organizer
      第一回環境DNA学会
    • Related Report
      2018 Research-status Report
  • [Book] 森林生態学2019

    • Author(s)
      石井弘明、德地直子、榎木 勉、名波 哲、廣部 宗、石丸香苗、榎木 勉、大園享司、長田典之、河村耕史、木庭啓介、小山里奈、杉浦真治、舘野隆之輔、菱 拓雄、保原 達、森 章
    • Total Pages
      184
    • Publisher
      朝倉書店
    • ISBN
      9784254470543
    • Related Report
      2019 Research-status Report
  • [Book] 森林生態学 5.1 水循環2019

    • Author(s)
      小山里奈(石井弘明ら編)
    • Total Pages
      184
    • Publisher
      朝倉書店
    • ISBN
      9784254470543
    • Related Report
      2018 Research-status Report
  • [Book] 生態系生態学(第2版) 第8章 植物の養分利用2018

    • Author(s)
      小山里奈(加藤知道ら監訳)
    • Total Pages
      608
    • Publisher
      森北出版
    • ISBN
      9784627261228
    • Related Report
      2018 Research-status Report
  • [Book] Carbon Sequestration in Mangroves (In: Blue carbon in Shallow Coastal Ecosystems: Carbon Dynamics, Policy, and Implementation)2018

    • Author(s)
      Tomomi Inoue (Kuwae T. and Hori M. eds)
    • Total Pages
      373
    • Publisher
      Springer
    • ISBN
      9789811312946
    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi