Reunion for the first time in 30 years - Challenge for experimental resource biology in sardine

Research Project

Project/Area Number	17K19295
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Research Field	Forestry and Forest Products Science, Applied aquatic science, and related fields
Research Institution	Kyushu University
Principal Investigator	MATSUYAMA Michiya 九州大学, 農学研究院, 教授 (00183955)
Research Collaborator	OHTA kohei YONEDA michio
Project Period (FY)	2017-06-30 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000) Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000) Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords	マイワシ / 生殖生理学 / 実験資源学 / 母性効果 / 環境応答
Outline of Final Research Achievements	Although Japanese sardines are one of the most important fishery resources in Japan, they do not spawn under rearing conditions, so details of reproductive characteristics and maternal effects were unknown. In this study, we aimed to develop a highly reproducible spawning induction technology as well as elucidating the cause of not spawning under rearing conditions in sardines. The present study succeeded in developing a highly reproducible spawning induction technology using sardines reared in a tank on land. In sardine females under rearing conditions, it was revealed that the absence of gonadotropin-releasing hormone (GnRH1) that promotes final oocyte maturation causes reproductive disorder.
Academic Significance and Societal Importance of the Research Achievements	水産資源の資源管理において重要な生物情報となる対象種の繁殖特性および母性効果の解明には、全生活史を再現する飼育実験系の開発が必要とされ、特に飼育下で産卵することが成否の鍵となる。本研究ではマイワシを対象として、飼育下で産卵しない原因を生殖生理学的に明らかにするとともに、飼育下で確実に産卵を誘導する技術を開発することに成功した。今後、本研究成果に基づき、マイワシの詳細な繁殖特性や母性効果に関する研究が急速に伸展することが期待される。