Development of detection schemes for endangered and invasive animals based on pan-animalia enviromental DNA analysis
| Project/Area Number |
17K19298
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Forestry and Forest Products Science, Applied aquatic science, and related fields
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
SATO Yukuto 琉球大学, 戦略的研究プロジェクトセンター, 特命講師 (20566418)
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 環境DNA / 大量DNA分析 / 次世代シークエンサー / バイオインフォマティクス / 希少種 / 外来種 / メタバーコーディング / 遺伝的多様性 / 生態調査 |
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| Outline of Final Research Achievements |
Methodological aspects of environmental DNA (eDNA) metabarcoding for vertebrates were investigated to detect tetrapods (amphibians, reptiles, avians, and mammals) from water samples. Using the Okinawa Island of Japan as a model system, I improved methods of water filtering, sample preservation, polymerase chain reaction (PCR) including choice of DNA polymerases and annealing temperatures, and data analysis. As a result, I achieved to detect eDNAs of endangered and invasive species of Okinawa Island which included Oryzias latipes, Babina holsti, Gallirallus okinawae, Poecilia reticulata, and Trachemys scripta. In addition, I showed that potential host animals of zoonotic pathogen Leptospira could be estimated based on the developed eDNA metabarcoding methods of river waters through correlated detection of pathogen bacterium and co-occurring vertebrate species.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究によって、河川水や海水から脊椎動物（魚類・四足類）の生息を検出する環境DNAメタバーコーディングの手法面が改良されたことで、環境DNA解析を、従来主な対象とされてきた魚類だけでなく、広く脊椎動物一般の検出と研究に活用していける可能性が示された。また本研究は、この環境DNA解析法を、人獣共通感染症の病原体の宿主となっている動物種の推定にも応用し得ることを論文成果として示した。今回、希少種や外来種を含む様々な脊椎動物を環境DNAで検出できたことを踏まえて、今後は検出の定量性や感度などを改良し、さらに遺伝的多様性推定の実現も目指すことで、保全生物学やメタ遺伝解析へ応用していくことが期待される。
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Report
(3 results)
Research Products
(14 results)
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[Journal Article] Environmental DNA enables detection of terrestrial mammals from forest pond water2017
Author(s)
Masayuki Ushio, Hisato Fukuda, Toshiki Inoue, Kobayashi Makoto, Osamu Kishida, Keiichi Sato, Koichi Murata, Masato Nikaido, Tetsuya Sado, Yukuto Sato, Masamichi Takeshita, Wataru Iwasaki, Hiroki Yamanaka, Michio Kondoh, and Masaki Miya
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Journal Title
Molecular Ecology Resources
Volume: 17(6):e
Issue: 6
Pages: 63-75
DOI
Related Report
Peer Reviewed
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[Presentation] Preliminary study on eDNA of the coelacanth's habitat around deep sea conservation areas of North Sulawesi, Indonesia2018
Author(s)
Kawilarang W.A. Masengi, I.F. Mandagi, H. Manengkey, P.N.I. Kalangi, A. Luasunaung, F.P.T. Pangalila, M. Iwata, Y. Abe, Y. Sato, R. Kimura, K. Yamahira
Organizer
第10回世界水族館会議
Related Report
Invited
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