• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of methane fermentation monitoring method integrated spectroscopy and metagenomics

Research Project

Project/Area Number 17K19313
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Agricultural economics and rural sociology, Agricultural Engineering, and related fields
Research InstitutionOsaka Prefecture University

Principal Investigator

Endo Ryosuke  大阪府立大学, 生命環境科学研究科, 講師 (10409146)

Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsケモメトリクス / メタゲノミクス / メタン発酵 / 診断技術 / 生物情報 / バイオマス / 資源循環 / 持続可能性 / 光センシング / 多変量解析 / 蛍光 / 機械学習 / 農業工学
Outline of Final Research Achievements

To diagnose the dynamics inside the methane fermentation process, fluorescence spectra and microbial genome inside methane fermentation digestate were acquired simultaneously and analyzed multivariately. As a result, methane fermentation characteristics such as pH and methane content in biogas were able to be accurately regressed by using partial least square method with input of a combination of fluorescence intensity and spectral reflection of a specific wavelength range. Furthermore, it was possible to diagnose the cause of the change in fermentation dinamicss by the substance related to the spectral reflection wavelength range or the fluorescence wavelength range and microorganism information used for the regression.

Academic Significance and Societal Importance of the Research Achievements

メタン発酵プロセスの代謝系は複雑であり,発酵阻害に対する処置はこれまで対症療法的で,根本原因の特定は困難だった.本研究で確立した分光・蛍光情報による発酵阻害の診断手法は,発酵阻害の原因を特定するといった原因療法が実現しうることを示している.メタン発酵は,有機性廃棄物からエネルギーと肥料成分を回収する資源化技術であり,社会の持続性向上に寄与する基幹技術として期待されている.本研究は,メタン発酵の安定的運転を可能とし,本技術のさらなる普及に貢献しうる.また,分光データと微生物情報のネットワーク解析は世界初の試みであり,多様な微生物群集と複雑な代謝系の変動を可視化例として学術的に重要な知見である.

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Application of Biogas Digestate as a Nutrient Solution for the Hydroponic Culture of Chrysanthemum morifolium Ramat with Rockwool Substrate2019

    • Author(s)
      Takemura Kenji、Endo Ryosuke、Shibuya Toshio、Kitaya Yoshiaki
    • Journal Title

      Waste and Biomass Valorization

      Volume: in press Issue: 6 Pages: 2645-2650

    • DOI

      10.1007/s12649-018-00576-8

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an in vitro hydroponic culture system for wasabi nursery plant production?Effects of nutrient concentration and supporting material on plantlet growth2019

    • Author(s)
      Hoang Nhung Ngoc、Kitaya Yoshiaki、Shibuya Toshio、Endo Ryosuke
    • Journal Title

      Scientia Horticulturae

      Volume: 245 Pages: 237-243

    • DOI

      10.1016/j.scienta.2018.10.025

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth and flowering responses of seed-propagated strawberry seedlings to different photoperiods in controlled environment chambers2018

    • Author(s)
      Tsuruyama J., Shibuya, T.
    • Journal Title

      HortTechnology

      Volume: 28 Pages: 453-458

    • DOI

      10.1016/j.scienta.2018.12.049

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of Methane through Anaerobic Digestion of Plant Residues in Tomato Production and Verification of Two Kinetic Models for This Process2017

    • Author(s)
      遠藤 良輔、福嶋 志隆
    • Journal Title

      Eco-Engineering

      Volume: 29 Issue: 3 Pages: 93-99

    • DOI

      10.11450/seitaikogaku.29.93

    • NAID

      130005874967

    • ISSN
      1347-0485, 1880-4500
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 吸光・蛍光センシングによるメタン発酵動態の診断2019

    • Author(s)
      遠藤良輔,真鍋尚哉,渋谷俊夫,北宅善昭
    • Organizer
      農業情報学会2019度年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光分光センシングとメタゲノミクスを統合したメタン発酵モニタリング手法の開発2018

    • Author(s)
      遠藤良輔,中口貴博,渋谷俊夫,北宅善昭
    • Organizer
      農業情報学会2018度年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Recovery of Energy Resources from Azolla Plants Using Anaerobic Digestion2017

    • Author(s)
      Ryosuke Endo, Kouki Nakamura, Nan Qin, Toshio Shibuya, Yoshiaki Kitaya
    • Organizer
      Eco-Engineering Symposium 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Improvement of anaerobic digestate for usage as nutrient solution in plant hydroponics2017

    • Author(s)
      Ryosuke Endo
    • Organizer
      CIGR World Workshop
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Calcium carbonate addition can facilitate the conversion of anaerobic digestate into hydroponic nutrient solution2017

    • Author(s)
      Ryosuke Endo, Masatoshi Maeda, Toshio Shibuya and Yoshiaki Kitaya
    • Organizer
      International symposium of greener cities
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 生物環境調節学研究グループ

    • URL

      http://envbio.envi.osakafu-u.ac.jp

    • Related Report
      2018 Annual Research Report 2017 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi