| Project/Area Number |
17K19322
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science, Animal science, and related fields
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| Research Institution | Okayama University |
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Principal Investigator |
KIMURA Koji 岡山大学, 環境生命科学研究科, 教授 (50355070)
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| Research Collaborator |
YAMAMOTO Yuki
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| Project Period (FY) |
2017-06-30 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 子宮腺 / 3次元培養 / ウシ / 分泌タンパク質 / 胚伸長 / 三次元培養 / 伸長胚 / 頂底極性 |
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| Outline of Final Research Achievements |
In this study, we established a method for isolation of bovine endometrial glands enzymatically and its culture system by embedding them in the matrigel. When the isolated uterine glands were cultured by this novel method, the gene expressions of the secretory proteins from bovine endometrium were significantly increased compared to those of glands cultured on plastic dishes (conventional culture method). Moreover, the glands cultured by this novel method had ability to react with sexual steroid hormones such as estradiol 17b and progesterone. The results of the present study are valuable for research on factors which affect the protein secretion ability of the uterine gland and contribute the study on improvement of fertility of cattle.
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| Academic Significance and Societal Importance of the Research Achievements |
ウシの妊娠継続には子宮内環境が重要であり、子宮腺がこれを支配する。子宮腺の機能を維持できる培養方法はこれまで開発されておらず、よって子宮腺機能については解明されていないことが多い。本課題では子宮腺をその構造を維持したまま回収する手法の開発に成功しただけでなく、従来法よりも優れた新規の培養方法を確立し、その有効性を示すことが出来た。この成果はウシの受胎性向上の技術開発につながる有用なものである。
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