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Research of the intrauterine environment and development of an artificial uterus apparatus for shark embryos

Research Project

Project/Area Number 17K19334
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science, Animal science, and related fields
Research InstitutionOkinawa Churashima Foundation

Principal Investigator

Sato Keiichi  一般財団法人沖縄美ら島財団(総合研究センター), 総合研究センター 動物研究室, 上席研究員 (80721745)

Project Period (FY) 2017-06-30 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsホホジロザメ / 繁殖 / 子宮 / 人工子宮 / 保全 / ヒレタカフジクジラ / 繁殖学 / ネズミザメ目 / 繁殖様式 / ネズミザメ / 人工哺育
Outline of Final Research Achievements

Studies on the uterine stuructures of sharks have been performed for understanding the diversity of reproductive mode in the Lamniformes and other matrotrophic sharks. For the moment, the lipid histotrophy was restricted in the early gestation stage of the white shark, and it could not be found in other lamniform species.
For futher understanding of the intrauterine environment, we developed an artificial uterus apparatus in preparation for the premature delivery of sharks with various reproduction modes. As a result of preliminary experiment of the deep-sea lantern shark, the world’s first successful long-term rearing over four months and artificial birth of the pups. Artificial rearing can match the reproduction modes of shark species with the goal of contributing to the understanding and conservation of many protected shark species.

Academic Significance and Societal Importance of the Research Achievements

サメ類の繁殖様式は極めて多様であり、その進化プロセスや生理学的な知見は極めて乏しい。本研究は、ホホジロザメに代表される胎生のサメ類にみられる子宮内の構造や環境に関する知見を集積し、子宮内環境の再現した人工子宮装置による人為的な哺育を目指している。また、その先には絶滅が危惧される大型サメ類の保全を見据え、将来の人為的な増殖を可能とする技術として、オプションの一つとしての選択肢となりうる。社会的な活動として、水族館施設を活用し、サメ類の多様な生態を最新の研究成果に基づき展示・解説することにより、海洋生物の多様性とその理解の重要性を普及啓発し、関心の高い話題を提供することができる。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (16 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (2 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (2 results) Remarks (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Pregnancy and Parturition: Teleost Fishes and Elasmobranchs2018

    • Author(s)
      Kawaguchi Mari、Sato Keiichi
    • Journal Title

      Encyclopedia of Reproduction Second Edition

      Volume: 6 Pages: 436-442

    • DOI

      10.1016/b978-0-12-809633-8.20590-6

    • ISBN
      9780128151457
    • Related Report
      2018 Research-status Report
  • [Journal Article] Live-bearing without placenta: Physical estimation indicates the high oxygen-supplying ability of white shark uterus to the embryo2017

    • Author(s)
      Tomita Taketeru、Nozu Ryo、Nakamura Masaru、Matsuzaki Shohei、Miyamoto Kei、Sato Keiichi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-7

    • DOI

      10.1038/s41598-017-11973-9

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Our mission: Improvement of aquarium functions to further contribute to society2019

    • Author(s)
      Keiichi Sato
    • Organizer
      中国自然博物館学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Reproduction in the Great White Shark Carcharodon carcharias, (1) Lipid histotrophy in early gestation2018

    • Author(s)
      Sato Keiichi, Nakamura Masaru, Tomita Taketeru
    • Organizer
      International Federation of Placenta Associations (IFPA) 2018 Meeting in Tokyo
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study of reproductive biology to create an “artificial uterus” to provide nutrients for great white shark embryos2018

    • Author(s)
      Sato Keiichi Sato, Tomita Taketeru Tomita, Nozu Ryo Nozu, Nakamura Masaru, Toda Minoru
    • Organizer
      The 10th International Aquarium Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ネズミザメ類の人工子宮作成を目指した繁殖学的研究2018

    • Author(s)
      佐藤圭一、冨田武照、野津了
    • Organizer
      日本板鰓類研究会 板鰓類シンポジウム2018
    • Related Report
      2018 Research-status Report
  • [Presentation] Oxygen-diffusion capacity of the white shark uterus: Oxygen delivery to an embryo without a placenta2017

    • Author(s)
      Tomita T, Nozu R, Nakamura M, Sato K.
    • Organizer
      Joint Meeting of Ichthyologists and Herpetologists
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Book] 寝てもサメても 深層サメ学2021

    • Author(s)
      佐藤 圭一、冨田 武照
    • Total Pages
      208
    • Publisher
      産業編集センター
    • ISBN
      9784863112988
    • Related Report
      2020 Annual Research Report
  • [Book] 科学の最前線を切りひらく!2020

    • Author(s)
      川端 裕人
    • Total Pages
      304
    • Publisher
      筑摩書房
    • ISBN
      9784480683724
    • Related Report
      2019 Research-status Report
  • [Remarks] 世界初!人工子宮装置をもちいた深海の発光ザメ「ヒレタカフジクジラ」胎仔の育成・人工出産に成功!

    • URL

      https://churaumi.okinawa/topics/1616482929/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 沖縄美ら島財団総合研究センター 動物研究室

    • URL

      https://churashima.okinawa/ocrc/marine_organisms/

    • Related Report
      2019 Research-status Report
  • [Remarks] 沖縄美ら海水族館 調査研究報告

    • URL

      https://churaumi.okinawa/research/

    • Related Report
      2019 Research-status Report
  • [Remarks] 沖縄美ら島財団総合研究センターHP: ジョーズの胎仔はなぜ子宮内で窒息死しない?その仕組みを解明

    • URL

      https://churashima.okinawa/ocrc/22/1366

    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Remarks] 沖縄美ら海水族館 調査研究活動報告

    • URL

      https://churaumi.okinawa/about/research/

    • Related Report
      2018 Research-status Report 2017 Research-status Report
  • [Remarks] OCRC HP: Research activity report

    • URL

      http://churashima.okinawa/en/ocrc/339/1387

    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 軟骨魚綱及び一部の硬骨魚綱海産魚類の大型胚仔飼育 方法及び飼育装置2021

    • Inventor(s)
      戸田実、冨田武照、佐藤圭一
    • Industrial Property Rights Holder
      一般財団法人 沖縄美ら島財団
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report

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Published: 2017-07-21   Modified: 2022-01-27  

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