| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
It is recently recognized that the functions of biomolecules (proteins and nucleic acids) are modulated by external forces (tension and compressive forces). Direct observation of proteins at work under an external load by high-speed AFM would greatly gain the mechanistic insight of their functional modulation under the load. To realize this observation, the present study have addressed the development of a high-speed AFM scanner with manipulator, in which a manipulation-scanner was additionally installed just adjacent to the imaging-scanner. The base of a manipulator needle was glued on the manipulation-scanner, while the other sharp end was gently placed on the surface of the imaging-scanner. Using the developed system, we succeeded in applying external force to biomolecules during high-AFM observation.
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