Direct observation of strain-dependent dynamic behaviors of biomolecules by high-speed AFM
| Project/Area Number |
17K19345
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Molecular and Genome biology and related fields
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
Kodera Noriyuki 金沢大学, ナノ生命科学研究所, 教授 (30584635)
|
|---|
| Project Period (FY) |
2017-06-30 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | 一分子イメージング / 高速AFM / 生体分子 / アクチン線維 / 細胞骨格 / 原子間力顕微鏡 / ナノ計測 / アクチンフィラメント / 生物物理学 / タンパク質 / モータータンパク質 / DNA結合タンパク質 / 生物物理 / 一分子計測（SMD) / 走査プローブ顕微鏡 |
|---|
| Outline of Final Research Achievements |
It is recently recognized that the functions of biomolecules (proteins and nucleic acids) are modulated by external forces (tension and compressive forces). Direct observation of proteins at work under an external load by high-speed AFM would greatly gain the mechanistic insight of their functional modulation under the load. To realize this observation, the present study have addressed the development of a high-speed AFM scanner with manipulator, in which a manipulation-scanner was additionally installed just adjacent to the imaging-scanner. The base of a manipulator needle was glued on the manipulation-scanner, while the other sharp end was gently placed on the surface of the imaging-scanner. Using the developed system, we succeeded in applying external force to biomolecules during high-AFM observation.
|
| Academic Significance and Societal Importance of the Research Achievements |
これまで生体分子の機能の張力応答は光ピンセット法などで調べることができたが、生体分子の形状がどのようになっているかは想像するしかなかった。一方、本研究の実施により、生体分子の形状と動きを高速AFM で同時観察しつつ、それらの張力負荷応答を直接的に見ることができる装置の開発が進められた。未だ誰も見たことがない生命現象の一端を理解するための重要な知見を提供する装置となることが期待される。また、生体分子の張力依存による機能変調を深く理解できれば、医薬品やバイオナノテクノロジーの開発指針となる新しい概念を提供できる可能性がある。
|
Report
(4 results)
Research Products
(87 results)
-
-
-
-
-
-
[Journal Article] Molecular and functional analysis of pore-forming toxin Monalysin from entomopathogenic bacterium Pseudomonas entomophila2020
Author(s)
2.Nonaka S†, Salim E†, Kamiya K*, Hori A, Nainu F, Asri RM, Masyita A, Nishiuchii T, Takeuchi S, Kodera N*, Kuraishi T*
-
Journal Title
Front. Immunol.
Volume: 11
Pages: 520-520
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
[Journal Article] Structural insights into the substrate specificity switch mechanism of the type III protein export apparatus2019
Author(s)
Y., Y. Ogawa, M. Kinoshita, N. Terahara, M. Shimada, N. Kodera, T. Ando, K. Namba, A. Kitao, K. Imada, T. Minamino
-
Journal Title
Structure
Volume: 印刷中
Issue: 6
Pages: 965-976
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
[Journal Article] Structure of the UHRF1 Tandem Tudor Domain Bound to a Methylated Non-histone Protein, LIG1, Reveals Rules for Binding and Regulation2019
Author(s)
Kori, S. Ferry, L. Matano, S. Jimenji, T. Kodera, N. Tsusaka, T. Matsumura, R. Oda, T. Sato, M. Dohmae, N. Ando, T. Shinkai, Y. Defossez, P. A. Arita, K.
-
Journal Title
Structure
Volume: 27
Issue: 3
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
[Journal Article] High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells.2017
Author(s)
Mohamed MS, Kobayashi A, Taoka A, Watanabe-Nakayama T, Kikuchi Y, Hazawa M, Minamoto T, Fukumori Y, Kodera N, Uchihashi T, Ando T, Wong RW
-
Journal Title
ACS Nano.
Volume: 11(6)
Issue: 6
Pages: 5567-5578
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Physical heterogeneity of bacterial membrane vesicles revealed by high-speed AFM2017
Author(s)
Yousuke Kikuchi, Tatunori Kiyokawa, Kana Morinaga, Yuuma Susa, Yasuda Marina, Hibiki Okuwaki, Ryukou Souma, Nozomu Obana, Masanori Toyohuku, Nobuhiko Nomura, Noriyuki Kodera, Toshio Ando, Yoshihiro Fukumori, Azuma Taoka
Organizer
第55回日本生物物理学会年会
Related Report
-
-
-
[Presentation] Loss of Nuclear Pore Selective Barrier Revealed by High-Speed Atomic Force Microscopy in Colorectal Cancer Cells2017
Author(s)
Mahmoud Shaaban Mohamed, Akiko Kobayashi, Azuma Taoka, Takahiro Watanabe-Nakayama, Yosuke Kikuchi, Masaharu Hazawa, Toshinari Minamoto, Yoshihiro Fukumori, Noriyuki Kodera, Takayuki Uchihashi, Toshio Ando, Richard Wong
Organizer
第55回日本生物物理学会年会
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
