| Budget Amount *help |
¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Outline of Final Research Achievements |
Halophilic archaea harbour three copies of 16S rRNA genes (rrsA and rrsBC) in their genomes. While rrsB and rrsC show almost identical sequences, rrsA shows 1-3% guanine-plus-cytosine content (PGC) difference compared to rrsBC. Based on the strong correlation between the PGC of 16S rRNA genes and the growth temperatures of the prokaryotes, we hypothesiszed that high-PGC rrsA and low-PGC rrsBC are expressed at high and low temperatures, respectively. The secondary structure prediction of the 16 rRNA via computer simulation showed that the structural stability of 16S rRNAs transcribed from rrsA was higher than that of 16S rRNAs transcribed from rrsBC. We measured expression levels of rrsA and rrsBC under various temperature conditions by reverse-transcriptase quantitative PCR. The expression ratio of high-PGC rrsA to low-PGC rrsBC increased with cultivation temperatures. Our results suggest that 16S rRNAs transcribed from high-PGC rrsA function under high temperature conditions.
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