| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In this research, we have developed two independent thermophilic E. coli strains by experimental thermo-adaptive evolution and analyzed them. Genome analyses showed that mutation rate of strains increased and peak out later in evolution.Interestingly, GC contents of genome DNA were gradually decreased with evolution. In the gene region of proteostasis network factors, mutation rate was about 3 times higher than in other regions.All 4 essential genes in proteostasis network were mutated in thermo-adaptive evolution. A part of mutations in essential genes of thermo-adapted E. coli included the change to the same amino acid with the homologous genes of thermophilic bacteria. These essential genes were global regulator genes. Mutation of groL (coded chaperonin GroEL) was throwback mutation in E. coli species evolution.
These results indicated that proteostasis network change was important for E. coli thermo-adaptive evolution.
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