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High-throughput analysis of embryo mechanical properties by atomic force microscopy

Research Project

Project/Area Number 17K19367
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Biology of Cells to Organisms, and related fields
Research InstitutionHokkaido University

Principal Investigator

okajima takaharu  北海道大学, 情報科学研究科, 教授 (70280998)

Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords細胞力学 / 発生胚 / 原子間力顕微鏡 / 生物物理 / 細胞
Outline of Final Research Achievements

Measuring cell mechanical properties is important to understand the mechanism of not only single cells but also cell population systems such as developing embryo. In this study, I developed a new type of atomic force microscopy (AFM) that allows us to map embryo mechanical properties at the single cell level. I first proposed an AFM method for calibrating the elastic modulus of cells with a large tilt angle. Next, I measured the stiffness of cell monolayer as a cell population system and found that the stiffness has a large spatial correlation in cell monolayer. Furthermore, I succeeded to measure the apparent Young's modulus and cell viscosity of embryo during the developing stages and found that the cell stiffness is associated with actin filaments and myosin in cortical regions. Overall, the AFM developed in this study is useful for investigating cell rheological properties in various types of embryo at the subcellular spatial resolution.

Academic Significance and Societal Importance of the Research Achievements

発生胚の胚形成メカニズムの理解は基礎生物学および再生医療において極めて重要である。近年、胚形成には細胞の力学特性が密接に関係していることが指摘されているが、胚形成過程における細胞力学特性を追跡する方法が皆無のため、胚発生の力学メカニズムは不明な点が多い、本研究では、原子間力顕微鏡を用いて、多数の発生胚の力学特性の計測を行った。本技術は、様々な発生胚の力学メカニズムの理解に貢献すると期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Spontaneous Spatial Correlation of Elastic Modulus in Jammed Epithelial Monolayers Observed by AFM2019

    • Author(s)
      Y. Fujii, Y. Ochi, M. Tuchiya, M. Kajita, Y. Fujita, Y. Ishimoto, T. Okajima
    • Journal Title

      Biophysical Journal

      Volume: 116 Issue: 6 Pages: 1152-1158

    • DOI

      10.1016/j.bpj.2019.01.037

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Calibrating the Young’s modulus of soft materials with surface tilt angle measured by atomic force microscopy2019

    • Author(s)
      Y. Fujii, T. Okajima
    • Journal Title

      AIP Advances

      Volume: 9 Issue: 1 Pages: 015028-015028

    • DOI

      10.1063/1.5046372

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Mapping cell stiffness of living embryo during the developmental process by atomic force microscopy2018

    • Author(s)
      Y. Fujii, T. Okajima
    • Organizer
      ACSIN-14&ICSPM26
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 原子間力顕微鏡による発生胚の弾性率のタイムラプス測定2018

    • Author(s)
      藤井裕紀、今井太一、小泉航、堀田耕司、岡浩太郎、岡嶋孝治
    • Organizer
      第79回応用物理学会秋季学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Spatiotemporal change in cell stiffness during early embryogenesis investigated by atomic force microscopy2018

    • Author(s)
      Y.Fujii, T. Imai, W. Koizumi, K. Hotta, K. Oka, and T.Okajima
    • Organizer
      62nd Annual Meeting Biophysical Society
    • Related Report
      2017 Research-status Report
  • [Presentation] Quantifying elastic modulus of soft samples with tilting angle by atomic force microscopy2017

    • Author(s)
      Y.Fujii and T.Okajima
    • Organizer
      25th International Colloquium on Scanning Probe Microscopy
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 初期胚発生過程における細胞弾性率と細胞骨格構造の時空間変動2017

    • Author(s)
      藤井 裕紀、今井 太一,小泉 航, 堀田 耕司, 岡 浩太郎, 岡嶋 孝治
    • Organizer
      第55回日本生物物理学会大会
    • Related Report
      2017 Research-status Report

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Published: 2017-07-21   Modified: 2020-03-30  

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