| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The function of neuron depends on axonal transport. Synaptic vesicles are transported by a kinesin superfamily protein KIF1A. Here, we tested a hypothesis that axonal transport is faster in giraffe than human because giraffe has a long axon along the long neck. By analyzing giraffe genome, we identified KIF1A sequence in silico. Based on the sequence, we synthesized the giraffe KIF1A cDNA. Using the cDNA, we expressed and purified giraffe KIF1A as well as human, worm and Drosophila KIF1A. We measured the velocity of these KIF1A and found that giraffe KIF1A is 1.5 times faster than human KIF1A.
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