Fabrication of biological graft by hyperbaric pressurization

• Project/Area Number: 17K19403
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Biology of Cells to Organisms, and related fields
• Research Institution: Tokyo Medical University (2019-2020); Yokohama City University (2017-2018)
• Principal Investigator: Yokoyama Utako 東京医科大学, 医学部, 主任教授 (70404994)
• Co-Investigator(Kenkyū-buntansha): 齋藤 純一 横浜市立大学, 医学研究科, 客員研究員 (30779301)
• Project Period (FY): 2017-06-30 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 再生医療 / 循環器 / メカニカルストレス / 細胞・組織 / バイオテクノロジー

Outline of Final Research Achievements

Researches on three-dimensional tissue construction methods have made remarkable progress, but the ideal vascular grafts that are strong enough against blood pressure have not been developed. In this study, we found that human vascular smooth muscle cells adhere to each other and produce extracellular matrices when subjected to extremely high pressures beyond the physiological range, resulting in the fabrication of vascular grafts. A rat smooth muscle cell-derived vascular graft produced by the same method could be transplanted into the rat aorta, and no rupture or thrombus was observed within a 5-month observation period.

Academic Significance and Societal Importance of the Research Achievements

ヒト血管平滑筋細胞に通常生体内で経験しないレベルの高圧力を印加することで、弾性線維をはじめとした三次元構造をもった細胞外基質を構築することができた。加圧印加培養を行うことで細胞のみから移植に耐えられる血管グラフトが作製できたことで、移植医療の技術となりえる可能性が示唆された。さらに非生理的範囲の周期的な静水圧印加による細胞応答を明らかにしたことで、細胞治療への学術的基盤知見となることが期待される。

Research Products

• [Journal Article] Scaffold-free tissue-engineered arterial grafts derived from human skeletal myoblasts. (2021)
  • Author(s): Saito J, Yokoyama U, Nakamura T, Kanaya T, Ueno T, Naito Y, Takayama T, Kaneko M, Miyagawa S, Sawa Y, and Ishikawa Y
  • Journal Title: Artif Organs Volume: - Issue: 8 Pages: 919-932
  • DOI: 10.1111/aor.13930
  • Peer Reviewed / Open Access
• [Journal Article] 血管の分化発達, 病態と再生 (2020)
  • Author(s): 横山詩子
  • Journal Title: 東京医科大学雑誌 Volume: 78 Pages: 8-15
• [Journal Article] Excessive EP4 signaling in smooth muscle cells induces abdominal aortic aneurysm by amplifying inflammation (2020)
  • Author(s): Hiromi T, Yokoyama U, Kurotaki D, Mamun A, Ishiwata R, Ichikawa Y, Nishihara H, Umemura M, Fujita T, Yasuda S, Minami T, Goda M, Uchida K, Suzuki S, Takeuchi I, Masuda M, Breyer M, Tamura T, and Ishikawa Y
  • Journal Title: Arteriosclerosis, Thrombosis, and Vascular Biology Volume: 40 Issue: 6 Pages: 1559-1573
  • DOI: 10.1161/atvbaha.120.314297
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fibulin-1 Integrates Subendothelial Extracellular Matrices and Contributes to Anatomical Closure of the Ductus Arteriosus. (2020)
  • Author(s): Ito S, Yokoyama U, Nakakoji T, Cooley MA, Sasaki T, Hatano S, Kato Y, Saito J, Nicho N, Iwasaki S, Umemura M, Fujita T, Masuda M, Asou T, and Ishikawa Y
  • Journal Title: Arterioscler Thromb Vasc Biol Volume: 40 Issue: 9 Pages: 2212-2226
  • DOI: 10.1161/atvbaha.120.314729
  • NAID: 130008001539
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of Tissue-Type Plasminogen Activator in Remodeling of the Ductus Arteriosus. (2020)
  • Author(s): Saito J, Ishikawa Y, Yokoyama U.
  • Journal Title: Circ Rep. Volume: in press
  • NAID: 130007830858
  • Peer Reviewed / Open Access
• [Journal Article] Antenatal Administration of Betamethasone Contributes to Intimal Thickening of the Rat Ductus Arteriosus (2019)
  • Author(s): Kemmotsu T., Yokoyama U., Saito J., Ito S., Uozumi A., Nishimaki S., Iwasaki S., Seki K., Ito S., Ishikawa Y
  • Journal Title: Circulation Journal Volume: 83 Issue: 3 Pages: 654-661
  • DOI: 10.1253/circj.CJ-18-1033
  • NAID: 130007604194
  • ISSN: 1346-9843, 1347-4820
  • Year and Date: 2019-02-25
  • Peer Reviewed / Open Access
• [Journal Article] Usefulness of Exchanged Protein Directly Activated by cAMP (Epac)1-Inhibiting Therapy for Prevention of Atrial and Ventricular Arrhythmias in Mice (2019)
  • Author(s): Prajapati R, Fujita T, Suita K, Nakamura T, Cai W, Hidaka Y, Umemura M, Yokoyama U, Knollmann BC, Okumura S, Ishikawa Y
  • Journal Title: Circulation Journal Volume: 83 Issue: 2 Pages: 295-303
  • DOI: 10.1253/circj.CJ-18-0743
  • NAID: 130007557004
  • ISSN: 1346-9843, 1347-4820
  • Year and Date: 2019-01-25
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reactive fibrosis precedes doxorubicin-induced heart failure through sterile inflammation. (2019)
  • Author(s): Tanaka R, Umemura M, Narikawa M, Hikichi M, Osaw K, Fujita T, Yokoyama U, Ishigami T, Tamura K, Ishikawa Y.
  • Journal Title: ESC Heart Fail. Volume: 7 Issue: 2 Pages: 588-603
  • DOI: 10.1002/ehf2.12616
  • Peer Reviewed / Open Access
• [Journal Article] Prostaglandin E2 receptor EP4 regulates cell migration through Orai1. (2019)
  • Author(s): Osawa K, Umemura M, Nakakaji R, Tanaka R, Islam RM, Nagasako A, Fujita T, Yokoyama U, Koizumi T, Mitsudo K, Ishikawa Y.
  • Journal Title: Cancer Sci. Volume: 111 Issue: 1 Pages: 160-174
  • DOI: 10.1111/cas.14247
  • Peer Reviewed / Open Access
• [Journal Article] Doxorubicin induces trans-differentiation and MMP1 expression in cardiac fibroblasts via cell death-independent pathways. (2019)
  • Author(s): Narikawa M, Umemura M, Tanaka R, Hikichi M, Nagasako A, Fujita T, Yokoyama U, Ishigami T, Kimura K, Tamura K, Ishikawa Y.
  • Journal Title: PLoS One. Volume: 14 Issue: 9 Pages: e0221940-e0221940
  • DOI: 10.1371/journal.pone.0221940
  • Peer Reviewed / Open Access
• [Journal Article] Functional expression of TRPM7 as a Ca(2+) influx pathway in adipocytes. (2019)
  • Author(s): Inoue H, Inazu M, Konishi M, Yokoyama U.
  • Journal Title: Physiol Rep. Volume: 7 Issue: 20
  • DOI: 10.14814/phy2.14272
  • Peer Reviewed / Open Access
• [Journal Article] Translationally controlled tumor protein (TCTP) plays a pivotal role in cardiomyocyte survival through a Bnip3-dependent mechanism. (2019)
  • Author(s): Cai W, Fujita T, Hidaka Y, Jin H, Suita K, Shigeta M, Kiyonari H, Umemura M, Yokoyama U, Sadoshima J, Ishikawa Y.
  • Journal Title: Cell Death Dis Volume: 10 Issue: 8 Pages: 549-549
  • DOI: 10.1038/s41419-019-1787-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Epac1 deficiency inhibits basic fibroblast growth factor-mediated vascular smooth muscle cell migration (2019)
  • Author(s): Yuko Kato, Utako Yokoyama, Takayuki Fujita, Masanari Umemura, Tetsuo Kubota, Yoshihiro Ishikawa
  • Journal Title: The Journal of Physiological Sciences Volume: 69 Issue: 2 Pages: 175-184
  • DOI: 10.1007/s12576-018-0631-7
  • NAID: 40021818894
  • Peer Reviewed / Open Access
• [Journal Article] An appropriately sized soft polyester external stent prevents enlargement and neointimal hyperplasia of a saphenous vein graft in a canine model (2019)
  • Author(s): Yasuda Shota、Goda Motohiko、Shibuya Taisuke、Uchida Keiji、Suzuki Shinichi、Noishiki Yasuharu、Yokoyama Utako、Ishikawa Yoshihiro、Masuda Munetaka
  • Journal Title: Artificial Organs Volume: 印刷中 Issue: 6 Pages: 577-583
  • DOI: 10.1111/aor.13399
  • Peer Reviewed / Open Access
• [Journal Article] Simultaneous hyperthermia-chemotherapy effect by arterial injection of Fe(Salen) for femur tumor (2018)
  • Author(s): Umemura Masanari、Islam Md. Rafikul、Fukumura Hidenobu、Sato Itaru、Kawabata Yusuke、Matsuo Kousuke、Nakakaji Rina、Nagasako Akane、Ohtake Makoto、Takayuki Fujita、Yokoyama Utako、Nakayama Tomohiro、Eguchi Haruki、Ishikawa Yoshihiro
  • Journal Title: Cancer Science Volume: 110 Issue: 1 Pages: 356-365
  • DOI: 10.1111/cas.13851
  • Peer Reviewed / Open Access
• [Journal Article] 動脈管閉鎖におけるt-PA活性の役割 (2018)
  • Author(s): 横山詩子, 齋藤純一, 石川義弘
  • Journal Title: 日本血栓止血学会誌 Volume: 29
• [Journal Article] Proteomic analysis of aortic smooth muscle cell secretions reveals an association of myosin heavy chain 11 with abdominal (2018)
  • Author(s): Yokoyama U, Arakawa N, Ishiwata R, Yasuda S, Minami T, Goda M, Uchida K, Suzuki S, Matsumoto M, Koizumi N, Taguri M, Hirano H, Yoshimura K, Ogino H, Masuda M, Ishikawa Y.
  • Journal Title: Am J Physiol Heart Circ Physiol. Volume: 315 Issue: 4 Pages: H1012-H1018
  • DOI: 10.1152/ajpheart.00329.2018
  • Peer Reviewed / Open Access
• [Journal Article] Hydrostatic pressure suppressed fibrotic change via Akt/GSK-3 signaling in human cardiac fibroblasts. (2018)
  • Author(s): Tanaka R, Umemura M, Narikawa M, Fujita T, Yokoyama U, Ishigami T, Kimura K, Tamura K, Ishikawa Y.
  • Journal Title: Physiol Rep. Volume: 6 Issue: 9 Pages: e13687-e13687
  • DOI: 10.14814/phy2.13687
  • Peer Reviewed / Open Access
• [Journal Article] Vidarabine, an anti-herpesvirus agent, prevents catecholamine-induced arrhythmias without adverse effect on heart function in mice (2018)
  • Author(s): Suita Kenji、Fujita Takayuki、Cai Wenqian、Hidaka Yuko、Jin Huiling、Prajapati Rajesh、Umemura Masanari、Yokoyama Utako、Sato Motohiko、Knollmann Bj?rn C.、Okumura Satoshi、Ishikawa Yoshihiro
  • Journal Title: Pfl?gers Archiv - European Journal of Physiology Volume: 未定 Issue: 6 Pages: 923-935
  • DOI: 10.1007/s00424-018-2121-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Acute hyperthermia inhibits TGF-β1-induced cardiac fibroblast activation via uppression of Akt Signaling. (2018)
  • Author(s): Narikawa M, Umemura M, Tanaka R, Fujita T, Yokoyama U, Ishigami T, Kimura K, Tamura K, Ishikawa Y.
  • Journal Title: Sci Rep. Volume: 8 Issue: 1 Pages: 6277-6277
  • DOI: 10.1038/s41598-018-24749-6
  • Peer Reviewed / Open Access
• [Journal Article] Fabrication of engineered tubular tissue for small blood vessels via three-dimensional cellular assembly and organization ex vivo (2018)
  • Author(s): Masuda T, Ukiki M, Yamagishi Y, Matsusaki M, Akashi M, Yokoyama U, and Arai F
  • Journal Title: J Biotechnol Volume: 20 Pages: 46-53
  • DOI: 10.1016/j.jbiotec.2018.04.003
  • Peer Reviewed / Open Access
• [Journal Article] A selective antagonist of prostaglandin E receptor subtype 4 attenuates abdominal aortic aneurysm. (2018)
  • Author(s): Mamun A, Yokoyama U, Saito J, Ito S, Hiromi T, Umemura M, Fujita T, Yasuda S, Minami T, Goda M, Uchida K, Suzuki S, Masuda M, Ishikawa Y.
  • Journal Title: Physiol Rep. Volume: 6 Issue: 18
  • DOI: 10.14814/phy2.13878
  • Peer Reviewed / Open Access
• [Journal Article] Alternating magnetic field (AMF) enhances cytotoxicity of compound c. (2018)
  • Author(s): Akimoto T, Umemura M, Nagasako A, Ohtake M, Fujita T, Yokoyama U, Eguchi H, Yamamoto T, Ishikawa Y.
  • Journal Title: Cancer Sci. Volume: 109 Issue: 11 Pages: 3483-3493
  • DOI: 10.1111/cas.13781
  • Peer Reviewed / Open Access
• [Journal Article] Treatment of oral cancer using magnetized paclitaxel (2018)
  • Author(s): Nakakaji Rina、Umemura Masanari、Mitsudo Kenji、Kim Jeong-Hwan、Hoshino Yujiro、Sato Itaru、Masuda Takatsugu、Yamamoto Masahiro、Kioi Mitomu、Koizumi Toshiyuki、Fujita Takayuki、Yokoyama Utako、Iida Masaki、Sato Motohiko、以下7名
  • Journal Title: Oncotarget Volume: 9 Issue: 21 Pages: 15591-15605
  • DOI: 10.18632/oncotarget.24570
  • Peer Reviewed / Open Access
• [Journal Article] The iron chelating agent, deferoxamine detoxifies Fe(Salen)-induced cytotoxicity (2017)
  • Author(s): Umemura Masanari、Kim Jeong-Hwan、Aoyama Haruki、Hoshino Yujiro、Fukumura Hidenobu、Nakakaji Rina、Sato Itaru、Ohtake Makoto、Akimoto Taisuke、Narikawa Masatoshi、Tanaka Ryo、Fujita Takayuki、Yokoyama Utako、Taguri Masataka、Okumura Satoshi、Sato Motohiko、Eguchi Haruki、Ishikawa Yoshihiro
  • Journal Title: Journal of Pharmacological Sciences Volume: 134 Issue: 4 Pages: 203-210
  • DOI: 10.1016/j.jphs.2017.07.002
  • Peer Reviewed / Open Access
• [Journal Article] L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor (2017)
  • Author(s): Masukawa Daiki、Koga Motokazu、Sezaki Anna、Nakao Yuka、Kamikubo Yuji、Hashimoto Tatsuo、Okuyama-Oki Yuki、Aladeokin Aderemi Caleb、Nakamura Fumio、Yokoyama Utako、Wakui Hiromichi、Ichinose Hiroshi、Sakurai Takashi、Umemura Satoshi、Tamura Koichi、Ishikawa Yoshihiro、Goshima Yoshio
  • Journal Title: JCI Insight Volume: 2 Issue: 18 Pages: 1-16
  • DOI: 10.1172/jci.insight.90903
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cardiac overexpression of Epac1 in transgenic mice rescues lipopolysaccharide-induced cardiac dysfunction and inhibits Jak-STAT pathway. (2017)
  • Author(s): Huiling Jin, Takayuki Fujita, Meihua Jin, Reiko Kurotani, Iyuki Namekata, Shogo Hamaguchi, Yuko Hidaka, Wenqian Cai, Kenji Suita, Yoshiki Ohnuki, Yasumasa Mototani, Kouichi Shiozawa, Rajesh Prajapati, Chen Liang, Masanari Umemura, Utako Yokoyama, Motohiko Sato, Hikaru Tanaka, Satoshi Okumura, Yoshihiro Ishikawa.
  • Journal Title: J Mol Cell Cardiol. Volume: 108 Pages: 170-180
  • DOI: 10.1016/j.yjmcc.2017.05.014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Attenuation of ductus arteriosus intimal thickening in preterm sheep twins compared with singletons (2017)
  • Author(s): Ito Satoko、Yokoyama Utako、Saito Junichi、Sato Shinichi、Usuda Haruo、Watanabe Shimpei、Kitanishi Ryuta、Miura Yuichiro、Saito Masatoshi、Hanita Takushi、Matsuda Tadashi、Ishikawa Yoshihiro
  • Journal Title: The Journal of Physiological Sciences Volume: 67 Issue: 6 Pages: 723-729
  • DOI: 10.1007/s12576-017-0565-5
  • NAID: 40021363517
  • Peer Reviewed / Open Access
• [Journal Article] Pathology and Molecular Mechanisms of Coarctation of the Aorta and its Association with the Ductus Arteriosus. (2017)
  • Author(s): Yokoyama U, Ichikawa Y, Minamisawa S, Ishikawa Y
  • Journal Title: J Physiol Sci. Volume: 67 Issue: 2 Pages: 259-270
  • DOI: 10.1007/s12576-016-0512-x
  • NAID: 40021113914
  • Peer Reviewed / Open Access
• [Journal Article] Epac activation inhibits IL-6-induced cardiac myocyte dysfunction. (2016)
  • Author(s): Jin H，Fujita T，Jin M，Kurotani R，Hidaka Y，Cai W，Suita K，Prajapati R，Liang C，Ohnuki Y，Mototani Y，Umemura M，Yokoyama U，Sato M，Okumura S，Ishikawa Y.
  • Journal Title: J Physiol Sci. Volume: 印刷中 Issue: 1 Pages: 77-87
  • DOI: 10.1007/s12576-016-0509-5
  • NAID: 40021443658
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transient receptor potential cation 3 channel regulates melanoma proliferation and migration． (2016)
  • Author(s): Oda K，Umemura M，Nakakaji R，Tanaka R，Sato I，Nagasako A，Oyamada C，Baljinnyam E，Katsumata M，Xie L，Narikawa M，Yamaguchi Y，Akimoto T，Ohtake M，Fujita T，Yokoyama U，Iwatsubo K，Aihara M and Ishikawa Y
  • Journal Title: J Physiol Sci. Volume: 該当なし Issue: 4 Pages: 497-505
  • DOI: 10.1007/s12576-016-0480-1
  • NAID: 40021238419
  • Peer Reviewed / Open Access
• [Presentation] Fabrication of tissue engineered blood vessel patch derived from umbilical smooth muscle cells. (2020)
  • Author(s): Kojima T, Yokoyama U, Yoda T, Nakamura T, Saito J, Miyagi E, and Ishikawa Y.
  • Organizer: The 72nd Annual Congress of the Japan Society of Obstetrics and Gynecology.
• [Presentation] Implantable tissue engineered blood vessels fabricated by periodic hydrostatic pressure (2020)
  • Author(s): Kojima T, Yokoyama U, Nakamura T, Saito J, Miyagi E, Ishikawa Y
  • Organizer: NIPS International Meeting on Cardiovascular Physiology
  • Int'l Joint Research
• [Presentation] Three-dimensional multilayers of smooth muscle cells acquire arterial physiology. (2020)
  • Author(s): Tanifuji S, Naito Y, Kojima T, Naruto T, Matsusaki M, and Yokoyama U
  • Organizer: NIPS International Meeting on Cardiovascular Physiology
  • Int'l Joint Research
• [Presentation] 臍帯平滑筋細胞を用いた血管グラフトパッチの作製. (2020)
  • Author(s): 小嶋朋之, 横山詩子, 中村隆, 齋藤純一, 宮城悦子, 石川義弘
  • Organizer: 第185回東京医科大学医学会総会
• [Presentation] 肺動脈性肺高血圧症における圧力のアポトーシスに及ぼす作用. (2020)
  • Author(s): 奥村祐輝, 加藤優子, 谷藤章太, and 横山詩子
  • Organizer: 第186回東京医科大学医学会総会
• [Presentation] 低酸素下で周期加圧を加えた臍帯動脈平滑筋細胞の特性について (2020)
  • Author(s): 小嶋朋之, 横山詩子, 中村隆, 齋藤純一, 宮城悦子, 石川義弘
  • Organizer: 第186回東京医科大学医学会総会
• [Presentation] 肺動脈性高血圧症におけるNotch3シグナル関連遺伝子の検討. (2020)
  • Author(s): 清水希来, 加藤優子, 谷藤章太, and 横山詩子
  • Organizer: 第186回東京医科大学医学会総会
• [Presentation] 動脈管分化の分子メカニズム (2020)
  • Author(s): 横山詩子
  • Organizer: 第125回日本解剖学会総会・全国学術集会
  • Invited
• [Presentation] Identification of ROS induced Na+-independent Mg2+ efflux in rat ventricular myocytes. (2020)
  • Author(s): Tashiro M, Inoue H, Konishi M, Yokoyama U
  • Organizer: The 97th Annual Meeting of the Physiological Society of Japan
• [Presentation] A new biomarker for abdominal aortic aneurysm. (2020)
  • Author(s): Yoshihiro I, Yokoyama U
  • Organizer: The 97th Annual Meeting of the Physiological Society of Japan
  • Invited
• [Presentation] Development of human arterial graft with mechanically functional extracellular matrices. (2020)
  • Author(s): Yokoyama U, Tomoyuki K, Junichi S, Takanori Y, Takashi N, Yoshinobu S, Kentaro K, Etsuko M, Yoshihiro I
  • Organizer: The 97th Annual Meeting of the Physiological Society of Japan
  • Invited
• [Presentation] The NH2-terminal region is the key for the differential drug sensitivity of TRPM7 and TRPM6. (2020)
  • Author(s): Inoue H, Murayama T, Kobayashi T, Mizumoto R, Yokoyama U
  • Organizer: The 97th Annual Meeting of the Physiological Society of Japan
• [Presentation] 異種細胞間の相互作用による生理的・病的血管リモデリングの分子機序 (2019)
  • Author(s): 横山詩子
  • Organizer: 東京医科歯科大学小児科マンデーセミナー
  • Invited
• [Presentation] Prostaglandin E receptor EP4-mediated regulation of vascular elasticity. (2019)
  • Author(s): Yokoyama U, Hiromi T, Ishikawa Y
  • Organizer: The 50th NIPS International Symposium MIRACLES In Cardiovascular Physiology
  • Int'l Joint Research / Invited
• [Presentation] Molecular Mechanisms of TRPM7 inhibition by oxidative stress. (2019)
  • Author(s): Inoue H, Murayama T, Konishi M, Tashiro M, Yokoyama U
  • Organizer: The 50th NIPS International Symposium MIRACLES In Cardiovascular Physiology
  • Int'l Joint Research
• [Presentation] プロスタグランジンＥによる弾性線維の制御機構と弾性を有する組織構築法の開発. (2019)
  • Author(s): 横山詩子
  • Organizer: 第4回エラスチン・関連分子研究会学術集会
  • Invited
• [Presentation] Role of endothelial cells in the ductus arteriosus remodeling. (2019)
  • Author(s): Saito J, Yokoyama U, Ishikawa Y
  • Organizer: The 3rd Taiwan-Korea-Japan Joint Congress on Neonatology
  • Invited
• [Presentation] 知っておきたい血管の病気「大動脈瘤」 (2019)
  • Author(s): 横山詩子
  • Organizer: 東京医科大学令和元年度後期健康講座
  • Invited
• [Presentation] 動脈硬化の進展に伴い血中ミオシン重鎖11は上昇する. (2019)
  • Author(s): 高橋梨紗, 石上友章, 冨山博文, 近森大志郎, 石川義弘, 横山詩子
  • Organizer: 第41回東京脈管研究会
• [Presentation] 動脈管におけるPDEⅢの役割と閉鎖のメカニズム. (2019)
  • Author(s): 横山詩子
  • Organizer: 第60回長野小児循環器談話会
  • Invited
• [Presentation] 血管の分化発達、病態と再生. (2019)
  • Author(s): 横山詩子
  • Organizer: 第184回東京医科大学医学会総会
  • Invited
• [Presentation] 活性酸素によるラット心室筋細胞内遊離マグネシウム濃度の低下. (2019)
  • Author(s): 吉森彩, 冨田煕, 田代倫子, 井上華, 小西真人, 横山詩子
  • Organizer: 第184回東京医科大学医学会総会
• [Presentation] EP4 signaling in smooth muscle cells causes abdominal aortic aneurysm by attraction of immune infiltrates. (2019)
  • Author(s): Hiromi T, Yokoyama U, Takeuchi I, Ishikawa Y
  • Organizer: The 92th Scientific Session, American Heart Association
  • Int'l Joint Research
• [Presentation] プロスタグランディンE受容体EP4と動脈瘤形成. (2019)
  • Author(s): 横山詩子
  • Organizer: NCVC第５回血管生理部セミナー
  • Invited
• [Presentation] プロスタグランディンE受容体EP4シグナルはLysyl oxydaseの発現を抑制し大動脈瘤の進行に関与する. (2019)
  • Author(s): 廣見太郎, 横山詩子, 竹内一郎, 石川義弘
  • Organizer: 第47回日本救急医学会総会・学術集会
• [Presentation] ニコチンの大動脈瘤発症に及ぼす役割ー動脈硬化と大動脈瘤の病態生理ー. (2019)
  • Author(s): 横山詩子, 藤田孝之, 梅村将就, 齋藤純一, 石川義弘
  • Organizer: 第34回喫煙科学研究財団平成30年度助成研究発表会
• [Presentation] Tissue-type plasminogen activator contributes to disruption of internal elastic lamina of the ductus arteriosus. (2019)
  • Author(s): Saito J, Yokoyama U, Ishikawa Y
  • Organizer: The Gordon Research Conference Elastin, Elastic fibers and Microfibrils
  • Int'l Joint Research
• [Presentation] 内皮細胞と平滑筋細胞の相互作用による血管リモデリング～動脈管解析からの新たな知見～ (2019)
  • Author(s): 横山詩子
  • Organizer: 第55回日本小児循環器学会総会・学術集会
  • Invited
• [Presentation] 内皮細胞が誘導する一方向性平滑筋細胞遊走が動脈管内膜肥厚を形成する. (2019)
  • Author(s): 伊藤智子、横山詩子、中川路太、齋藤純一、二町尚樹、益田宗孝、麻生俊英、石川義弘
  • Organizer: 第55回日本小児循環器学会総会・学術集会
• [Presentation] ロスタグランディンE受容体EP4と血管の病態生理. (2019)
  • Author(s): 横山詩子
  • Organizer: 第4回日本肺高血圧・肺循環学会学術集会
  • Invited
• [Presentation] 動脈管平滑筋細胞間由来Fibulin-1は内皮由来Versicanと共役して動脈管内膜肥厚形成を促進する. (2019)
  • Author(s): 伊藤智子、横山詩子、石川義弘
  • Organizer: 第51回日本結合組織学会学術大会
• [Presentation] 動脈管の閉鎖機序と今後の治療の展開. (2019)
  • Author(s): 横山詩子
  • Organizer: 東京慈恵会医科大学大学小児科カンファレンス
  • Invited
• [Presentation] Gaseous components of cigarette smoke upregulate prostaglandin E2 receptor EP4 in aortic aneurysm (2019)
  • Author(s): Hiromi T, Yokoyama U, Takeuchi I, and Ishikawa Y
  • Organizer: The 9th Federation of the Asian and Oceanian Physiological Societies Congress
  • Int'l Joint Research / Invited
• [Presentation] へその緒から作る人工血管 心臓病の子供たちに成長する人工血管を届けたい (2019)
  • Author(s): 齋藤純一、横山詩子
  • Organizer: 市民講座「細胞エクササイズで頑丈な人工血管を作ろう！」
  • Invited
• [Presentation] 細胞をトレーニングしたら頑丈な血管ができた！細胞から血管を作る加圧トレーニングのレシピを発見 (2019)
  • Author(s): 横山詩子
  • Organizer: 市民講座「細胞エクササイズで頑丈な人工血管を作ろう！」
  • Invited
• [Presentation] 動脈管は面白い！100年前からの謎を解く (2019)
  • Author(s): 横山詩子
  • Organizer: 横浜市立大学小児科リサーチ発表会
  • Invited
• [Presentation] 動脈管の内皮細胞による血管リモデリング (2019)
  • Author(s): 齋藤純一 and 横山詩子
  • Organizer: 第13回 新生児科指導医教育セミナー
  • Invited
• [Presentation] 静水圧印加による血管系組織の再生 (2018)
  • Author(s): 横山詩子
  • Organizer: 都医学研セミナー
  • Invited
• [Presentation] How ”Human arterial graft” should be fabricated? (2018)
  • Author(s): Yokoyama U
  • Organizer: CBS 2018- IEEE International Conference on Cyborg and Bionic Systems
  • Int'l Joint Research / Invited
• [Presentation] 動脈管作動薬の作用機序ー薬物療法の基礎として (2018)
  • Author(s): 横山詩子
  • Organizer: 第10回教育セミナー Advanced Course 主催：日本小児循環器学会学術委員会・教育委員会
  • Invited
• [Presentation] 三次元血管モデルの有用性 (2018)
  • Author(s): 横山詩子
  • Organizer: オープンイノベーションカンファレンスIII
  • Invited
• [Presentation] 動脈管閉鎖における内皮機能の役割 (2018)
  • Author(s): 齋藤純一、横山詩子、益田宗孝、麻生俊英、石川義弘
  • Organizer: 第54回日本小児循環器学会総会・学術集会
  • Invited
• [Presentation] 肺高血圧に合併した肺動脈瘤におけるEP4 の発現とその意義 (2018)
  • Author(s): 赤木達、中村一文、横山詩子、笠原真悟、更科俊洋、江尻健太郎、伊藤浩
  • Organizer: 第3回日本肺高血圧・肺循環学会学術集会
  • Invited
• [Presentation] 周期加圧培養法による人工血管の作成 (2018)
  • Author(s): 石川義弘、横山詩子、齋藤純一、金子真
  • Organizer: 第39回日本循環制御医学会総会
  • Invited
• [Presentation] 周期的加圧培養による移植可能な血管グラフトの作製 (2018)
  • Author(s): 齋藤純一、横山詩子、高山俊男、洞出光洋、伊藤弘明、須郷慶信、倉澤健太郎、宮城悦子、金子真、石川義弘
  • Organizer: 第17回日本心臓血管発生研究会
• [Presentation] 母体ラットへのべタメタゾン投与が動脈管内膜肥厚に与える作用の検討 (2018)
  • Author(s): 釼持孝博、横山詩子、齋藤純一、伊藤智子、魚住梓、岩崎志穂、西巻滋、関和男、伊藤秀一、石川義弘
  • Organizer: 第63回日本新生児成育医学科医・学術集会
• [Presentation] プロスタグランディンE-EP4シグナルによる大動脈瘤進行の分子機序 (2018)
  • Author(s): 横山詩子, 廣見太郎, and 石川義弘
  • Organizer: 心血管膜輸送研究会2018
• [Presentation] Mechanoresponse to periodic hydrostatic pressure enables fabrication of human arterial graft (2018)
  • Author(s): Saito J, Yokoyama U, Takayama T, Horade M, Ito H, Sugo Y, Kurasawa K, Ogawa M, Miyagi E, Kaneko M, and Ishikawa Y
  • Organizer: American Society for Matrix Biology Biennial Meeting 2018
  • Int'l Joint Research
• [Presentation] プロスタグランディンE受容体EP4シグナルは腹部大動脈瘤の増悪に関与する (2018)
  • Author(s): 廣見太郎, 横山詩子, Al M, 竹内一郎, and 石川義弘
  • Organizer: CCM輸液・栄養研究会
• [Presentation] Prostaglandin E2 promotes vascular remodeling of the ductus arteriosus (2018)
  • Author(s): Minamisawa S, Akaike T, and Yokoyama U
  • Organizer: The 25th Euro Congress on Pediatric Heart Disease and Health
  • Int'l Joint Research
• [Presentation] Fibulin-1の動脈管内膜肥厚形成における役割 (2018)
  • Author(s): 中川路太一、横山詩子、伊藤智子、石川義弘
  • Organizer: 第28回日本病態生理学会大会
• [Presentation] 胎盤由来プロスタグランディンEはFibulin-1を介した動脈管リモデリングを促進する (2018)
  • Author(s): 横山詩子、伊藤智子、石川義弘
  • Organizer: 第50回日本結合組織学会学術大会
• [Presentation] Tissue-type plasminogen activator promotes intimal thickening formation of the ductus arteriosus (2018)
  • Author(s): Saito J, Yokoyama U, Nicho N, Zheng YW, Ito S, Ito S, Taniguchi H, Asou T, Masuda M, and Ishikawa Y
  • Organizer: Weinstein 2018 Cardiovascular Development and Regeneration Conference
  • Int'l Joint Research
• [Presentation] Antenatal administration of betamethasone promotes closure of the preterm ductus arteriosus via intimal thickening formation (2018)
  • Author(s): Kemmotsu T, Yokoyama U, Azuma S, Saito J, Ito S, Uozumi A, Nishimaki S, Iwasaki S, Seki K, Ito S, and Ishikawa Y
  • Organizer: Weinstein 2018 Cardiovascular Development and Regeneration Conference
  • Int'l Joint Research
• [Presentation] Prostaglandin E-EP4 signaling-mediated fibulin-1 integrates extracellular matrices to promote smooth muscle cell migration of the ductus arteriosus (2018)
  • Author(s): Ito S, Yokoyama U, Saito JZ, Y.W., Masuda M, Asou T, and Ishikawa Y
  • Organizer: Weinstein 2018 Cardiovascular Development and Regeneration Conference
  • Int'l Joint Research
• [Presentation] Fabrication of scaffold-free human vascular graft by periodic hydrostatic pressurization (2018)
  • Author(s): Yokoyama, U., Saito, J., Ito, H., Tadokoro, T., Taniguchi, H., Kaneko, M. Ishikawa, Y.
  • Organizer: The 95th Annual Meeting of the Physiological Society of Japan
  • Invited
• [Presentation] 肺血管の発達と密接な関連を有する動脈管の発達 (2018)
  • Author(s): 横山詩子
  • Organizer: 第２４回日本小児肺循環研究会
  • Invited
• [Presentation] Tissue-type plasminogen activator contributes to remodeling of the ductus arteriosus (2018)
  • Author(s): Saito, J., Yokoyama, U. Ishikawa, Y.
  • Organizer: The 95th Annual Meeting of the Physiological Society of Japan
• [Presentation] Epac1 deficiency inhibits bFGF-induced neointimal formation via diminished phosphorylation of GSK3beta (2018)
  • Author(s): Kato, Y., Yokoyama, U., Fujita, T., Kubota, T. Ishikawa, Y.
  • Organizer: The 95th Annual Meeting of the Physiological Society of Japan
• [Presentation] Molecular mechanisms of the ductus arteriosus (2017)
  • Author(s): Yokoyama, U
  • Organizer: The 90th Scientific Session, American Heart Association
  • Int'l Joint Research / Invited
• [Presentation] New insights on how to treat patent ductus arteriosus (2017)
  • Author(s): Yokoyama, U
  • Organizer: The 8th TAKAO International symposium
  • Int'l Joint Research / Invited
• [Presentation] プロスタグランディンE受容体EP4による弾性線維形成と破壊のメカニズム (2017)
  • Author(s): 横山詩子、石渡遼、石川義弘.
  • Organizer: 第４９回日本結合組織学会
  • Invited
• [Presentation] 動脈管の基礎研究から臨床へ (2017)
  • Author(s): 横山詩子、南沢享、石川義弘.
  • Organizer: 第120回日本小児科学会学術集会
  • Invited
• [Presentation] プロスタグランディンE受容体EP4による血管の分化と破壊のメカニズム (2017)
  • Author(s): 横山詩子
  • Organizer: 横浜市立大学生命医科学研究科連携セミナー
  • Invited
• [Presentation] 知っておきたい大動脈瘤の進行のしくみ～世界初の治療薬開発に向けて～ (2017)
  • Author(s): 横山詩子
  • Organizer: 第49回先端医科学研究センター市民講座
  • Invited
• [Presentation] Fabrication of Implantable Human Arterial Graft by Periodic Hydrostatic Pressure (2017)
  • Author(s): Saito, J., Yokoyama, U., Ito, H., Tadokoro, T., Sugo, Y., Kurasawa, K., Ogawa, M., Miyagi, E., Taniguchi, H., Kaneko, M. Ishikawa, Y.
  • Organizer: The 8th TAKAO International symposium
  • Int'l Joint Research
• [Presentation] Antenatal Administration of Betamethasone Contributes to Intimal thickening of the Ductus Arteriosus (2017)
  • Author(s): Kemmotsu, T., Yokoyama, U., Azuma, S., Saito, J., Ito, S., Uozumi, A., Iwasaki, S., Nishimaki, S., Ito, S., Masuda, M., Asou, T.Ishikawa,
  • Organizer: The 8th TAKAO International symposium
  • Int'l Joint Research
• [Presentation] Prostaglandin E-EP4-mediated fibulin-1 up-regulation plays a role in intimal thickening of the ductus arteriosus (2017)
  • Author(s): Ito, S., Yokoyama, U., Saito, J., Masuda, M., Asou, T.Ishikawa, Y.
  • Organizer: The 8th TAKAO International symposium
  • Int'l Joint Research
• [Presentation] 母体ラットへのベタメタゾン投与が動脈管閉鎖に与える作用の検討 (2017)
  • Author(s): 釼持孝博、横山詩子、齋藤純一、魚住梓、岩崎志穂、西巻滋、伊藤秀一、石川義弘.
  • Organizer: 第53回日本周産期・新生児医学会学術集会
• [Presentation] Fibulin-1 の動脈管内膜肥厚形成機序 (2017)
  • Author(s): 伊藤智子、横山詩子、石川義弘.
  • Organizer: 第53回日本周産期・新生児医学会学術集会
• [Presentation] 多胎妊娠では動脈管内膜肥厚が抑制される (2017)
  • Author(s): 伊藤智子、横山詩子、齋藤純一、佐藤信一、臼田治夫、渡邊真平、北西龍太、三浦雄一郎、埴田卓志、松田直、石川義弘.
  • Organizer: 第62回日本新生児成育医学会学術集会
• [Book] 動脈管開存症 (2021)
  • Author(s): 南沢享、横山詩子
  • Total Pages: 323
  • Publisher: メジカルビュー社
  • ISBN: 9784758319690
• [Book] 動脈管の発生から閉鎖およびその異常 (2021)
  • Author(s): 横山詩子、南沢享
  • Total Pages: 323
  • Publisher: メジカルビュー社
  • ISBN: 9784758319690
• [Book] New insights on how to treat patent ductus arteriosus. (2020)
  • Author(s): Yokoyama U, Aoki R, Fujita S, Iwasaki S, Seki K, Toshihide A, Masuda M, Minamisawa S, Ishikawa Y.
  • Total Pages: 398
  • Publisher: Springer
  • ISBN: 9789811511851
• [Book] Fabrication of implantable human arterial graft by periodic hydrostatic pressure. (2020)
  • Author(s): Saito J, Yokoyama U, Takayama T, Ito H, Tadokoro T, Sugo Y, Kurasawa K, Ogawa M, Miyagi E, Taniguchi H, Kaneko M, Ishikawa Y.
  • Total Pages: 398
  • Publisher: Springer
• [Book] Antenatal administration of betamethasone contributes to intimal thickening of the ductus arteriosus. (2020)
  • Author(s): Kemmotsu T, Yokoyama U, Saito J, Ito S, Uozumi A, Iwasaki S, Nishimaki S, Ito S, Masuda M, Asou T, Ishikawa Y.
  • Total Pages: 398
  • Publisher: Springer
• [Book] Prostaglandin E-EP4-mediated fibulin-1 up-regulation plays a role in intimal thickening of the ductus arteriosus. (2020)
  • Author(s): Ito S, Yokoyama U, Saito J, Masuda M, Toshihide A, Ishikawa Y.
  • Total Pages: 398
  • Publisher: Springer
• [Book] 小児・成育循環器学. 動脈管作用薬 (2018)
  • Author(s): 横山詩子
  • Total Pages: 724
  • Publisher: 診断と治療社
  • ISBN: 9784787822888
• [Book] ガイトン生理学 原著第13 版 第８４章：胎児と新生児の生理学 (2018)
  • Author(s): 横山詩子、齋藤純一
  • Total Pages: 1100
  • Publisher: エルゼビア・ジャパン社
• [Book] ガイトン生理学 原著第13 版 第１５章：動脈系と静脈系の血管伸展性と機能 (2018)
  • Author(s): 横山詩子
  • Total Pages: 1100
  • Publisher: エルゼビア・ジャパン社
• [Book] ガイトン生理学 原著第13 版 第１４章：循環の概要；圧，流量，抵抗の生物物理学 (2018)
  • Author(s): 横山詩子
  • Total Pages: 1100
  • Publisher: エルゼビア・ジャパン社
• [Book] 新版 先天性心疾患を理解するための臨床心臓発生学 動脈管閉鎖の分子機構 (2018)
  • Author(s): 横山詩子、南沢享
  • Publisher: メジカルビュー社
• [Book] 新版 先天性心疾患を理解するための臨床心臓発生学 動脈管開存症 (2018)
  • Author(s): 横山詩子、南沢享
  • Publisher: メジカルビュー社
• [Book] 小児循環器学 動脈管作用薬 (2018)
  • Author(s): 横山詩子
  • Publisher: 診断と治療社
• [Remarks] 東京医科大学細胞生理学分野
  • URL: https://tokyo-med-physiology.jimdofree.com/
• [Remarks] 横浜市立大学医学部循環制御医学
  • URL: http://www-user.yokohama-cu.ac.jp/~seiri1/index.html
• [Patent(Industrial Property Rights)] Method for producing three-dimensional cell aggregates. (2020)
  • Inventor(s): Yokoyama U, et al.
  • Industrial Property Rights Holder: Yokoyama U, et al.
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020
  • Acquisition Date: 2020
  • Overseas