Diversity and evolution of eukaryotic pinocytosis

• Project/Area Number: 17K19434
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Ecology and Evolution (including anthropology), and related fields
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: YABUKI Akinori 国立研究開発法人海洋研究開発機構, 地球環境部門(海洋生物環境影響研究センター), 研究員 (20711104)
• Co-Investigator(Kenkyū-buntansha): 石田 健一郎 筑波大学, 生命環境系, 教授 (30282198)
• Project Period (FY): 2017-06-30 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 真核微生物 / 飲作用 / 吸収栄養 / 微細構造 / 多様性 / 新規系統 / 浸透栄養 / 環境配列 / ディプロネマ綱 / ケルコゾア門 / 系統分類 / 飲作用（ピノサイトーシス）

Outline of Final Research Achievements

The diversity and evolution of pinocytosis that is a biological function to absorb the nutrients via small vesicle formation from outside have been studied focusing microbial eukaryotes. Although little was known about the diversity of microbial eukaryotes that can survive relying on pinocytosis only, we showed that it is common in various eukaryotic lineages, especially in Cercozoa and Diplonemea. On the other hand, the process and places of the vesicle formation in the cells and the dependence on pinocytosis for the growth are different even between closely related species. Our achievements totally contributed to estimate the ancestral state of nutrient acquisition of the eukaryotes and its diversification process: the pinocytosis itself has been equipped at the very early stage of eukaryotic evolution (at the emergence of eukaryotic cell, probably) and the places of the small vesicle formation has been diversified lineages by lineages.

Academic Significance and Societal Importance of the Research Achievements

本課題によってもたらされた知見は、未だ謎が多い真核細胞の成立とその初期進化過程を理解する上で極めて重要な意味を持つ。また多くの系統で浸透栄養に依存した原生生物が認識された事実は、水圏生態系の構造やエネルギーの流れをより正確に理解する上でも重要かつ新たな意味を持つ。飲作用における小胞形成過程に共通性及び系統ごとの特殊性が確認されたことは、真核細胞機能の多様性とその進化の複雑性を示している。そこからは真核生物初期進化をより詳細に理解するための発展的基礎研究の道が拓けただけでなく、細胞機能に着目した生理医学的研究の基盤情報としての価値も期待される。

Research Products

• [Journal Article] Inventory and Evolution of Mitochondrion-localized Family A DNA Polymerases in Euglenozoa (2020)
  • Author(s): Ryo Harada, Yoshihisa Hirakawa, Akinori Yabuki, Yuichiro, Kashiyama, Moe Maruyama, Onuma Ryo, Petr Soukal, Shinya Miyagishima, Vladimir Hampl, Goro Tanifuji, Yuji Inagaki
  • Journal Title: Pathogens Volume: 9 Issue: 4 Pages: 257-257
  • DOI: 10.3390/pathogens9040257
  • NAID: 120007127758
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Morphology, ultrastructure, and phylogeny of two novel species of Ventrifissura (V. oblonga n. sp. and V. velata n. sp., Thecofilosea, Cercozoa) (2020)
  • Author(s): Takashi Shiratori, Akinori Yabuki, Ken-ichiro Ishida
  • Journal Title: Protist Volume: - Issue: 3 Pages: 125731-125731
  • DOI: 10.1016/j.protis.2020.125731
  • Peer Reviewed
• [Journal Article] Morphological, Ultrastructural, Motility and Evolutionary Characterization of Two New Hemistasiidae Species (2019)
  • Author(s): Prokopchuk Galina、Tashyreva Daria、Yabuki Akinori、Horak Ales、Masarova Petra、Lukes Julius
  • Journal Title: Protist Volume: - Issue: 3 Pages: 259-282
  • DOI: 10.1016/j.protis.2019.04.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ophirina amphinema n. gen., n. sp., a New Deeply Branching Discobid with Phylogenetic Affinity to Jakobids (2018)
  • Author(s): Yabuki Akinori、Gyaltshen Yangtsho、Heiss Aaron A.、Fujikura Katsunori、Kim Eunsoo
  • Journal Title: Scientific Reports Volume: 8 Issue: 1
  • DOI: 10.1038/s41598-018-34504-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phylogeny and Morphology of New Diplonemids from Japan (2018)
  • Author(s): Tashyreva Daria、Prokopchuk Galina、Yabuki Akinori、Kaur Binnypreet、Faktorova Drahomira、Votypka Jan、Kusaka Chiho、Fujikura Katsunori、Shiratori Takashi、Ishida Ken-Ichiro、Horak Ales、Lukes Julius
  • Journal Title: PROTIST Volume: 169 Issue: 2 Pages: 158-179
  • DOI: 10.1016/j.protis.2018.02.001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Life Cycle, Ultrastructure, and Phylogeny of New Diplonemids and Their Endosymbiotic Bacteria (2018)
  • Author(s): Tashyreva Daria、Prokopchuk Galina、Votypka Jan、Yabuki Akinori、Horak Ales、Lukes Julius
  • Journal Title: mBio Volume: 9 Issue: 2
  • DOI: 10.1128/mbio.02447-17
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 非光合成性藻類におけるclpC遺伝子の有無を介した色素体ゲノム喪失に対する機能的制約 (2020)
  • Author(s): 加山 基、矢吹彬憲、宮下英明、神川龍馬
  • Organizer: 日本藻類学会第44回大会
• [Presentation] エンドへレア太陽虫Microheliella marisの系統的位置とミトコンドリアゲノム (2020)
  • Author(s): 矢吹彬憲，矢崎裕規，白鳥峻志，今泉朱夏，久米慶太郎，橋本哲男，稲垣祐司
  • Organizer: 日本藻類学会第44回大会
• [Presentation] 新規ケルコゾア生物YPF1514株（テコフィローセア綱）の分類学的研究 (2019)
  • Author(s): 藤井千早、白鳥崚志、矢吹彬憲
  • Organizer: 第52回日本原生生物学会
• [Presentation] ユーグレノゾアにおけるミトコンドリア局在DNAポリメラーゼの進化 (2019)
  • Author(s): 原田 亮、平川泰久、矢吹彬憲、柏山祐一郎、谷藤吾郎、大沼 亮、Vladimir Hampl、稲垣祐司
  • Organizer: 第52回日本原生生物学会
• [Presentation] 原生生物嗅覚学はじめました。 (2018)
  • Author(s): 矢吹 彬憲，伊左治 雄太，菅 寿美，伊東 薫，中村 多実子，大河内 直彦，藤倉 克則
  • Organizer: 第51回日本原生生物学会大会
• [Presentation] 吸収栄養性ケルコゾア生物の驚くべき多様性 (2018)
  • Author(s): 矢吹彬憲、白鳥峻志、藤井千早、藤倉克則
  • Organizer: 日本微生物生態学会第32回大会
• [Presentation] Orphan protistology, accelerating in JAPAN (2018)
  • Author(s): Takashi Shiratori, Akinori Yabuki, Euki Yazaki, Yuki Nishimura, Moriya Ohkuma, Katsunori Fujikura, Tetsuo Hashimoto, Yuji Inagaki, Ken-ichiro Ishida
  • Organizer: 第2回日韓合同原生生物学会
  • Int'l Joint Research