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Exploration of subsurface microbial electrosphere

Research Project

Project/Area Number 17K19435
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Ecology and Evolution (including anthropology), and related fields
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

Ishii Shunichi  国立研究開発法人海洋研究開発機構, 海底資源研究開発センター, 研究員 (10556913)

Project Period (FY) 2017-06-30 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords電気微生物 / 発電菌 / 電気メタン合成菌 / 微生物燃料電池 / メタトランスクリプトミクス / メタゲノム / 地下圏微生物 / 発電微生物 / 電気合成微生物 / 微生物電気メタン合成 / 地下電気生命圏 / 掘削コア / 微生物電気合成 / 菌体外電子輸送 / 電極還元菌 / 電気共生 / 生物電気化学リアクター / 刺激応答型メタトランスクリプトーム
Outline of Final Research Achievements

In this research, we explored “electrogenic microbes that donate electrons to solids and breathe” and “electrotrophic microbes that use electricity as energy source” from various environmental microbiome by accumulation cultures in the bioelectrochemical reactors. As a result, acetate or hydrogen is an important substrate for electrogenic microbes. As for the electrogenic microbes, Geoalkalibacter spp. were enriched from seawater environment, while Geobacter spp. were enriched from fleshwater environment. In addition, electrometotrophic microbes of the genera Methanocalculus and Methanobacterium were observed in the reactors that filled with seawater-based subsurface groundwater. The MetaOMICs analyses revealed metabolic functions of the electrogenic microbes that affiliated with Geobacter subsurface clade, and their electron transfer pathways.

Academic Significance and Societal Importance of the Research Achievements

本研究の結果、地下圏において電気に依存して生きる微生物が新たに発見された。また、海水性の条件と汽水性の条件で異なる電気微生物が見つかった。電気微生物のある一群は、固体表面の電極電位に対して多様な応答を見せる事が明らかになった。これらの結果は、異なる表面電極電位を有する鉱物や、酸化還元電位勾配を有する地下環境に適応し、多種多様な電気微生物が生息している事を示唆している。本研究により、電気生命圏を形成しうる様々な電気微生物群が地下環境に存在している事が判明し、未だに知られていない地下圏微生物の生き様に関する新たな知見を加える事ができた。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Bioelectrochemical Stimulation of Electromethanogenesis at a Seawater-Based Subsurface Aquifer in a Natural Gas Field2019

    • Author(s)
      Ishii Shun'ichi、Imachi Hiroyuki、Kawano Kenjiro、Murai Daisuke、Ogawara Miyuki、Uemastu Katsuyuki、Nealson Kenneth H.、Inagaki Fumio
    • Journal Title

      Frontiers in Energy Research

      Volume: 6 Pages: 144-144

    • DOI

      10.3389/fenrg.2018.00144

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative metatranscriptomics reveals extracellular electron transfer pathways conferring microbial adaptivity to surface redox potential changes2018

    • Author(s)
      Ishii Shun’ichi、Suzuki Shino、Tenney Aaron、Nealson Kenneth H.、Bretschger Orianna
    • Journal Title

      The ISME Journal

      Volume: 12 Issue: 12 Pages: 2844-2863

    • DOI

      10.1038/s41396-018-0238-2

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 発電微生物群集中の発電菌とその機能遺伝子2018

    • Author(s)
      石井俊一
    • Journal Title

      環境バイオテクノロジー学会誌

      Volume: 18 Pages: 35-42

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metagenomic insights into the ecology and physiology of microbes in bioelectrochemical systems2018

    • Author(s)
      Kouzuma Atsushi、Ishii Shun'ichi、Watanabe Kazuya
    • Journal Title

      Bioresource Technology

      Volume: 255 Pages: 302-307

    • DOI

      10.1016/j.biortech.2018.01.125

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] メタトランスクリプトーム解析によって解き明かす電気微生物の表面電極電位変化への応答2018

    • Author(s)
      石井俊一
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Bioelectrochemical stimulation of methanogenic microbial communities in a seawater-based subsurface aquifer system2017

    • Author(s)
      S. Ishii, H. Imachi, K. Kawano, D. Murai, F. Inagaki
    • Organizer
      5th international meeting on microbial electrochemistry and technologies (ISMET6)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] システムゲノミクスで解き明かす“発電微生物”の多様な生き様2017

    • Author(s)
      石井俊一
    • Organizer
      環境微生物系学会合同大会
    • Related Report
      2017 Research-status Report
    • Invited

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Published: 2017-07-21   Modified: 2020-03-30  

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