A study of operational mechanisms in neural circuits based on activity measurement of whole synapses in the brain
| Project/Area Number |
17K19439
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Neuroscience and related fields
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
Nose Akinao 東京大学, 大学院新領域創成科学研究科, 教授 (30260037)
|
|---|
| Project Period (FY) |
2017-06-30 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
|---|
| Keywords | ショウジョウバエ / カルシウムイメージング / 神経回路 / シナプス / 神経活動ダイナミクス |
|---|
| Outline of Final Research Achievements |
To comprehend the dynamics in complicated neural circuits, we developed a novel method for large scale synapse imaging and examined circuit mechanisms for generating functional neural dynamics. We expressed a membrane bound-form calcium sensor in the central nervous system of Drosophila larvae and performed time-lapse fluorescence imaging. We analyzed the spatiotemporal fluorescence data by clustering based on statistical physics, extraction of network structure using transfer entropy, and architecture analysis by graph theory. In the end, we succeeded in identifying a novel circuit module underlying generation of multiple patterns of larval locomotion.
|
| Academic Significance and Societal Importance of the Research Achievements |
中枢神経系において、多様な神経機能を生み出している実体はシナプスであるが、これまでシナプス集団の活動ダイナミクスは技術的な難しさから明らかになっていなかった。本研究では、シナプス活動のイメージングプローブの開発と、時系列データから回路構造を推定する新規解析手法の構築によって、異なる運動パターン生成の背後にある回路モジュールの同定に成功した。この成果により、複雑な神経回路から、シナプス集団の活動を追跡し統計的解析を施すことによって、回路に潜む構造を見出すことが可能であることが示され、これにより、神経回路ダイナミクスの定量解析の可能性が大きく拓かれたと考えられる。
|
Report
(4 results)
Research Products
(38 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Divergent connectivity of homologous command-like neurons mediates segment-specific touch responses in Drosophila2017
Author(s)
Suguru Takagi, Benjamin T. Cocanougher, Sawako Niki, Dohjin Miyamoto, Hiroshi Kohsaka, Hokto Kazama, Richard D. Fetter, James W. Truman, Marta Zlatic, Albert Cardona, Akinao Nose.
Organizer
CSHLMeeting "Neurobiology of Drosophila"
Related Report
Int'l Joint Research
-
[Presentation] Divergent connectivity of command-like neurons mediates segment-specific touch responses in Drosophila larvae2017
Author(s)
高木 優, Benjamin Cocanougher, 二木 佐和子, 宮本 道人, 高坂 洋史, 風間 北斗, Richard Fetter, James Truman, Marta Zlatic, Albert Cardona, 能瀬 聡直
Organizer
第17回 東京大学生命科学シンポジウム
Related Report
-
[Presentation] Bidirectional feedback circuits regulate propagation of motor activity in opposite directions2017
Author(s)
Kohsaka, H., Zwart, M.F., Fushiki, A., Fetter, R. D., Truman, J. W., Cardona, A. and Nose, A.
Organizer
第40回日本神経科学大会
Related Report
-
-
[Presentation] A segment-specific sensorimotor pathway mediates touch-triggered backward escape response in Drosophila larvae2017
Author(s)
Suguru Takagi, Benjamin T. Cocanougher, Sawako Niki, Dohjin Miyamoto, Hiroshi Kohsaka, Hokto Kazama, Richard D. Fetter, James W. Truman, Marta Zlatic, Albert Cardona, Akinao Nose
Organizer
第40回日本神経科学大会
Related Report
-
-
-
-
-
-
-
-
-
