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A study of operational mechanisms in neural circuits based on activity measurement of whole synapses in the brain

Research Project

Project/Area Number 17K19439
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Research Field Neuroscience and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Nose Akinao  東京大学, 大学院新領域創成科学研究科, 教授 (30260037)

Project Period (FY) 2017-06-30 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywordsショウジョウバエ / カルシウムイメージング / 神経回路 / シナプス / 神経活動ダイナミクス
Outline of Final Research Achievements

To comprehend the dynamics in complicated neural circuits, we developed a novel method for large scale synapse imaging and examined circuit mechanisms for generating functional neural dynamics. We expressed a membrane bound-form calcium sensor in the central nervous system of Drosophila larvae and performed time-lapse fluorescence imaging. We analyzed the spatiotemporal fluorescence data by clustering based on statistical physics, extraction of network structure using transfer entropy, and architecture analysis by graph theory. In the end, we succeeded in identifying a novel circuit module underlying generation of multiple patterns of larval locomotion.

Academic Significance and Societal Importance of the Research Achievements

中枢神経系において、多様な神経機能を生み出している実体はシナプスであるが、これまでシナプス集団の活動ダイナミクスは技術的な難しさから明らかになっていなかった。本研究では、シナプス活動のイメージングプローブの開発と、時系列データから回路構造を推定する新規解析手法の構築によって、異なる運動パターン生成の背後にある回路モジュールの同定に成功した。この成果により、複雑な神経回路から、シナプス集団の活動を追跡し統計的解析を施すことによって、回路に潜む構造を見出すことが可能であることが示され、これにより、神経回路ダイナミクスの定量解析の可能性が大きく拓かれたと考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (38 results)

All 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (5 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (23 results) (of which Int'l Joint Research: 13 results,  Invited: 9 results) Book (1 results) Remarks (2 results)

  • [Int'l Joint Research] University of Cambridge(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Washington(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of St Andrews(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Freiburg(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Janelia Farm Research Campus(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University of Washington(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University of St Andrews(英国)

    • Related Report
      2018 Research-status Report
  • [Journal Article] System level analysis of motor-related neural activities in larval Drosophila2019

    • Author(s)
      Yoon Youngteak、Park Jeonghyuk、Taniguchi Atsushi、Kohsaka Hiroshi、Nakae Ken、Nonaka Shigenori、Ishii Shin、Nose Akinao
    • Journal Title

      Journal of Neurogenetics

      Volume: 33 Issue: 3 Pages: 179-189

    • DOI

      10.1080/01677063.2019.1605365

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of forward and backward locomotion through intersegmental feedback circuits in Drosophila larvae2019

    • Author(s)
      Kohsaka Hiroshi、Zwart Maarten F.、Fushiki Akira、Fetter Richard D.、Truman James W.、Cardona Albert、Nose Akinao
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41467-019-10695-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Data-driven analysis of motor activity implicates 5-HT2A neurons in backward locomotion of larval Drosophila2018

    • Author(s)
      Park Jeonghyuk、Kondo Shu、Tanimoto Hiromu、Kohsaka Hiroshi、Nose Akinao
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1-15

    • DOI

      10.1038/s41598-018-28680-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Divergent Connectivity of Homologous Command-like Neurons Mediates Segment-Specific Touch Responses in Drosophila2017

    • Author(s)
      Takagi Suguru、Cocanougher Benjamin Thomas、Niki Sawako、Miyamoto Dohjin、Kohsaka Hiroshi、Kazama Hokto、Fetter Richard Doty、Truman James William、Zlatic Marta、Cardona Albert、Nose Akinao
    • Journal Title

      Neuron

      Volume: 96 Issue: 6 Pages: 1373-1387.e6

    • DOI

      10.1016/j.neuron.2017.10.030

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 光によるショウジョウバエ中枢回路の機能解剖2017

    • Author(s)
      能瀬聡直、高坂洋史、伏木彬
    • Journal Title

      生体の科学

      Volume: 68(5) Pages: 478-479

    • Related Report
      2017 Research-status Report
  • [Presentation] Functional connectomics of motor circuits in Drosophila larvae2019

    • Author(s)
      Nose A
    • Organizer
      Max Planck / HHMI Connectomics Conference Berlin 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Experience-driven development of motor circuits in Drosophila2019

    • Author(s)
      Zeng X, Kawasaki T, Inada K, Kazama H, Nose A
    • Organizer
      52th Annual Meeting of the Japanese Society of Developmental Biologists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Embryonic development of the motor circuits in Drosophila: emergence of coordinated neural activities and the role of sensory feedback2019

    • Author(s)
      Zeng X, Kawasaki T, Inada K, Kazama H, Nose A
    • Organizer
      第42回日本神経科学大会
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Neural activities related to axial locomotion in the terminal segments of Drosophila larval CNS2019

    • Author(s)
      Y. Liu, A. Nose and H. Kohsaka
    • Organizer
      2019 ASIA-PACIFIC DROSOPHILA NEUROBIOLOGY CONFERENCE
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regulation of motor circuit development by proprioceptive feedback2019

    • Author(s)
      A. Nose
    • Organizer
      2019 ASIA-PACIFIC DRORSOPHILA NEUROBIOLOGY CONFERENCE
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation of embryonic motor circuit formation by somatosensory feedback2018

    • Author(s)
      A. Nose, X. Zeng, K. Inada and H. Kazama
    • Organizer
      The Joint Research Area Meeting Scientific Research on Innovative Areas. “Artificial Intelligence and Brain Science” and “Adaptive Circuit Shift”
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] A modular structure in premotor circuits for bidirectional axial locomotion2018

    • Author(s)
      H. Kohsaka, M.F. Zwart, A. Fushiki, R.D. Fetter, J.W. Truman, A. Cardona, and A. Nose
    • Organizer
      Behavioral neurogenetics of Drosophila larva
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Optogenetic and connectome analyses of the neural circuits regulating escape behaviors in Drosophila larvae2018

    • Author(s)
      S. Takagi and A. Nose
    • Organizer
      第10回光操作研究会・第2回脳情報動態 合同国際シンポジウム
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Embryonic development of the motor circuits in Drosophila: emergence of coordinated neural activities and the role of sensory feedback2018

    • Author(s)
      X. Zeng, T. Kawasaki, K. Inada, H. Kazama and A. Nose
    • Organizer
      Behavioral Neurogenetics of Drosophila Larva
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Neural circuits regulating divergent escape responses in Drosophila larvae: implication for evo-devo of adaptive behaviors2018

    • Author(s)
      S. Takagi and A. Nose
    • Organizer
      The 41st Annual Meeting of the Molecular Biology Society of Japan
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Circuit mechanisms of action selection in Drosophila larvae, Systems biology of the brain2017

    • Author(s)
      Akinao Nose
    • Organizer
      “Reconstructing the connectome of the fruit fly larva” : CUSO Staromics PhD-program Workshop
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Optophysiological and connectome analyses of the neural circuits regulating escape behaviors in Drosophila larvae2017

    • Author(s)
      Akinao Nose
    • Organizer
      日本比較生理生化学会第39回福岡大会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Divergent connectivity of homologous command-like neurons mediates segment-specific touch responses in Drosophila2017

    • Author(s)
      Suguru Takagi, Benjamin T. Cocanougher, Sawako Niki, Dohjin Miyamoto, Hiroshi Kohsaka, Hokto Kazama, Richard D. Fetter, James W. Truman, Marta Zlatic, Albert Cardona, Akinao Nose.
    • Organizer
      CSHLMeeting "Neurobiology of Drosophila"
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Divergent connectivity of command-like neurons mediates segment-specific touch responses in Drosophila larvae2017

    • Author(s)
      高木 優, Benjamin Cocanougher, 二木 佐和子, 宮本 道人, 高坂 洋史, 風間 北斗, Richard Fetter, James Truman, Marta Zlatic, Albert Cardona, 能瀬 聡直
    • Organizer
      第17回 東京大学生命科学シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] Bidirectional feedback circuits regulate propagation of motor activity in opposite directions2017

    • Author(s)
      Kohsaka, H., Zwart, M.F., Fushiki, A., Fetter, R. D., Truman, J. W., Cardona, A. and Nose, A.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Identification of interneurons that regulate backward locomotion during light-avoidance behavior in Drosophila larvae2017

    • Author(s)
      Shoya Ohura, Hiroshi Kohsaka, Akinao Nose
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] A segment-specific sensorimotor pathway mediates touch-triggered backward escape response in Drosophila larvae2017

    • Author(s)
      Suguru Takagi, Benjamin T. Cocanougher, Sawako Niki, Dohjin Miyamoto, Hiroshi Kohsaka, Hokto Kazama, Richard D. Fetter, James W. Truman, Marta Zlatic, Albert Cardona, Akinao Nose
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Identification of neuronal circuitry that regulate backward escape behavior in Drosophila larvae2017

    • Author(s)
      Atsuki Hiramoto, Julius Jonaitis, Sawako Niki, Dohjin Miyamoto, Richard Fetter, Albert Cardona, Stefan Pulver, Akinao Nose.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ショウジョウバエ幼虫の光逃避行動において後退運動を制御する介在神経細胞の同定2017

    • Author(s)
      Shoya Ohura, Hiroshi Kohsaka, Akinao Nose
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] ショウジョウバエ近縁種における幼虫行動特性の比較解析2017

    • Author(s)
      松尾悠司、能瀬聡直、高坂洋史
    • Organizer
      第40回日本分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Sensory feedback regulates the development of locomotor circuits in Drosophila embryos.2017

    • Author(s)
      xiangsunze zeng, Tappei Kawasaki, Akinao Nose
    • Organizer
      第40回日本分子生物学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Sensory feedback regulates the development of locomotor circuits in Drosophila embryos.2017

    • Author(s)
      xiangsunze zeng, Tappei Kawasaki, Akinao Nose.
    • Organizer
      Behavioral adaptation and functional recovery from pathological states
    • Related Report
      2017 Research-status Report
  • [Presentation] Sensory feedback regulates the development of locomotor circuits in Drosophila embryos.2017

    • Author(s)
      xiangsunze zeng, Tappei Kawasaki, Akinao Nose.
    • Organizer
      次世代脳プロジェクト冬のシンポジウム
    • Related Report
      2017 Research-status Report
  • [Book] Neuroscience2019

    • Author(s)
      S. Takagi and A. Nose
    • Total Pages
      6
    • Publisher
      Circuit architecture for somatotopic action selection in Invertebrates
    • Related Report
      2018 Research-status Report
  • [Remarks] 能瀬・高坂研究室

    • URL

      http://bio.phys.s.u-tokyo.ac.jp/

    • Related Report
      2019 Annual Research Report 2017 Research-status Report
  • [Remarks] 能瀬・高坂研究室

    • URL

      http://bio.phys.s.u-tokyo.ac.jp/index.html

    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-07-21   Modified: 2021-02-19  

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