Development of a comprehensive tag-insertion method that enables dynamic in vivo analysis

• Project/Area Number: 17K19444
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Neuroscience and related fields
• Research Institution: Niigata University
• Principal Investigator: Sakimura Kenji 新潟大学, 脳研究所, フェロー (40162325)
• Co-Investigator(Kenkyū-buntansha): 阿部 学 新潟大学, 脳研究所, 准教授 (10334674)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 生体分子標識 / ペプチドタグ / ノックインマウス / 遺伝子編集 / 神経科学 / 分子タグ / マウス

Outline of Final Research Achievements

The purpose of this study is to develop a new method for accurately detecting protein molecules that function in vivo, and to apply this method to a group of molecules working in the nervous system. We adopted a system combining a low-molecular-weight chemical probe, instead of an antibody, and a peptide tag that recognizes the probe. Thus we successfully developed a method for rapidly inserting a peptide tag to a site that is not expected to affect the function of a target molecule, using a gene editing technique. With this method, we have succeeded in establishing a mouse whose GluN1 molecules are knocked in with an hD2 tag bound by a gold particle-labeled probe that can visualize the subunit structure of the NMDA-type glutamate receptor under an electron microscope.

Academic Significance and Societal Importance of the Research Achievements

本研究では、低分子量のケミカルプローブを認識するペプチドタグを、遺伝子編集法により迅速に脳研究に適したマウスに組み込む方法の開発に成功した。この成果により、１）これまで抗体ができなかった分子の解析が可能になる。２）低分子のケミカルプローブは組織内への浸透性が高いので、これまでできなかった生体での分子動態解析などが可能になる。第3に、高輝度のケミカルプローブを用いることで、細胞内での分子動態の可視化が可能になる。したがって、本研究開発の成果は、生命科学、とりわけ脳機能解析に大きな変化をもたらす。

Research Products

• [Int'l Joint Research] Institute of Science and Technology(オーストリア)
• [Int'l Joint Research] DANDRITE, Aarhus University(デンマーク)
• [Int'l Joint Research] IST Austria(オーストリア)
• [Journal Article] Mechanical regulation of oligodendrocyte morphology and maturation by the mechanosensor p130Cas (2019)
  • Author(s): Shimizu Takeshi、Osanai Yasuyuki、Tanaka Kenji F.、Thai Truc Quynh、Abe Manabu、Natsume Rie、Sakimura Kenji、Ikenaka Kazuhiro
  • Journal Title: Journal of Neurochemistry Volume: 2019 Issue: 2 Pages: 158-172
  • DOI: 10.1111/jnc.14657
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Input-specific NMDAR-Dcpendent potentiation of dendritic GABAergic inhibition. (2018)
  • Author(s): Chiu CQ, Martenson JS, Yamazaki M, Natsume R, Sakimura K, Tomita S, Tavalin SJ, Higley MJ.
  • Journal Title: Ncuron. Volume: 97 Issue: 2 Pages: 368-377
  • DOI: 10.1016/j.neuron.2017.12.032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Targeting the SphK1/S1P/S1PR1 Axis That Links Obesity, Chronic Inflammation, and Breast Cancer Metastasis (2018)
  • Author(s): Nagahashi Masayuki、Yamada Akimitsu、Katsuta Eriko、Aoyagi Tomoyoshi、Huang Wei-Ching、Terracina Krista P.、Hait Nitai C.、Allegood Jeremy C.、Tsuchida Junko、Yuza Kizuki、Nakajima Masato、Abe Manabu、Sakimura Kenji、Milstien Sheldon、Wakai Toshifumi、Spiegel Sarah、Takabe Kazuaki
  • Journal Title: Cancer Research Volume: 78 Issue: 7 Pages: 1713-1725
  • DOI: 10.1158/0008-5472.can-17-1423
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum (2018)
  • Author(s): Uesaka Naofumi、Abe Manabu、Konno Kohtarou、Yamazaki Maya、Sakoori Kazuto、Watanabe Takaki、Kao Tzu-Huei、Mikuni Takayasu、Watanabe Masahiko、Sakimura Kenji、Kano Masanobu
  • Journal Title: Neuron Volume: 97 Issue: 4 Pages: 796-805
  • DOI: 10.1016/j.neuron.2018.01.018
  • Peer Reviewed / Open Access
• [Journal Article] Microglia permit climbing fiber elimination by promoting GABAergic inhibition in the developing cerebellum (2018)
  • Author(s): Nakayama Hisako、Abe Manabu、Morimoto Chie、Iida Tadatsune、Okabe Shigeo、Sakimura Kenji、Hashimoto Kouichi
  • Journal Title: Nature Communications Volume: 9 Issue: 1 Pages: 2830-2830
  • DOI: 10.1038/s41467-018-05100-z
  • Peer Reviewed / Open Access
• [Journal Article] Deletion of exons encoding carboxypeptidase domain of Nna1 results in Purkinje cell degeneration (pcd) phenotype (2018)
  • Author(s): Zhou Li、Hossain M. Ibrahim、Yamazaki Maya、Abe Manabu、Natsume Rie、Konno Kohtaro、Kageyama Shun、Komatsu Masaaki、Watanabe Masahiko、Sakimura Kenji、Takebayashi Hirohide
  • Journal Title: Journal of Neurochemistry Volume: 147 Issue: 4 Pages: 557-572
  • DOI: 10.1111/jnc.14591
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Deficiency of AMPAR?Palmitoylation Aggravates Seizure Susceptibility (2018)
  • Author(s): Itoh Masayuki、Yamashita Mariko、Kaneko Masaki、Okuno Hiroyuki、Abe Manabu、Yamazaki Maya、Natsume Rie、Yamada Daisuke、Kaizuka Toshie、Suwa Reiko、Sakimura Kenji、Sekiguchi Masayuki、Wada Keiji、Hoshino Mikio、Mishina Masayoshi、Hayashi Takashi
  • Journal Title: The Journal of Neuroscience Volume: 38 Issue: 47 Pages: 10220-10235
  • DOI: 10.1523/jneurosci.1590-18.2018
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of Caskin1 protein and phenotypic characterization of its knockout mice using a comprehensive behavioral test battery (2018)
  • Author(s): Katano Tayo、Takao Keizo、Abe Manabu、Yamazaki Maya、Watanabe Masahiko、Miyakawa Tsuyoshi、Sakimura Kenji、Ito Seiji
  • Journal Title: Molecular Brain Volume: 11 Issue: 1 Pages: 63-63
  • DOI: 10.1186/s13041-018-0407-2
  • Peer Reviewed / Open Access
• [Journal Article] PRRT2 deficiency induces paroxysmal kinesigenic dyskinesia by regulating synaptic transmission in cerebellum. (2018)
  • Author(s): Tan GH, Liu YY, Wang L, Li K, Zhang ZQ, Li HF, Yang ZF, Li Y, Li D, Wu MY, Yu CL, Long JJ, Chen RC, Li LX, Yin LP, Liu JW, Cheng XW, Shen Q, Shu YS, Sakimura K, Liao LJ, Wu ZY, Xiong ZQ.
  • Journal Title: Cell Res. Volume: 28 Issue: 1 Pages: 90-110
  • DOI: 10.1038/cr.2017.128
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CDC42EP4, a perisynaptic scaffold protein in Bergmann glia, is required for glutamatergic tripartite synapse configuration. (2018)
  • Author(s): Ageta-Ishihara N, Konno K, Yamazaki M, Abe M, Sakimura K, Watanabe M, Kinoshita M.
  • Journal Title: Neurochem Int Volume: S0197-0186 Issue: 17 Pages: 30442-4
  • DOI: 10.1016/j.neuint.2018.01.003
  • Peer Reviewed / Open Access
• [Journal Article] Keratan Sulfate Phenotype in the β-1,3-N-Acetylglucosaminyltransferase-7?Null Mouse Cornea (2018)
  • Author(s): Littlechild Stacy L.、Young Robert D.、Caterson Bruce、Yoshida Hideyuki、Yamazaki Maya、Sakimura Kenji、Quantock Andrew J.、Akama Tomoya O.
  • Journal Title: Investigative Opthalmology & Visual Science Volume: 59 Issue: 3 Pages: 1641-1641
  • DOI: 10.1167/iovs.17-22716
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The number and distribution of AMPA receptor channels containing fast kinetic GluA3 and GluA4 subunits at auditory nerve synapses depend on the target cells (2017)
  • Author(s): Rubio Mar?a E.、Matsui Ko、Fukazawa Yugo、Kamasawa Naomi、Harada Harumi、Itakura Makoto、Moln?r Elek、Abe Manabu、Sakimura Kenji、Shigemoto Ryuichi
  • Journal Title: Brain Structure and Function Volume: 222 Issue: 8 Pages: 3375-3393
  • DOI: 10.1007/s00429-017-1408-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Purkinje Cells Are More Vulnerable to the Specific Depletion of Cathepsin D Than to That of Atg7 (2017)
  • Author(s): Koike Masato、Shibata Masahiro、Sunabori Takehiko、Yamaguchi Junji、Sakimura Kenji、Komatsu Masaaki、Tanaka Keiji、Uchiyama Yasuo
  • Journal Title: The American Journal of Pathology Volume: 187 Issue: 7 Pages: 1586-1600
  • DOI: 10.1016/j.ajpath.2017.02.020
  • Peer Reviewed
• [Journal Article] Atg9a deficiency causes axon-specific lesions including neuronal circuit dysgenesis. (2017)
  • Author(s): Yamaguchi J, Suzuki C, Nanao T, Kakuta S, Ozawa K, Tanida I, Saitoh T, Sunabori T, Komatsu M, Tanaka K, Aoki S, Sakimura K, Uchiyama Y.
  • Journal Title: Autophagy Volume: 17 Issue: 5 Pages: 1-14
  • DOI: 10.1080/15548627.2017.1314897
  • NAID: 120006881392
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Unconventional role of voltage-gated proton channels (VSOP/Hv1) in regulation of microglial ROS production (2017)
  • Author(s): Kawai Takafumi、Okochi Yoshifumi、Ozaki Tomohiko、Imura Yoshio、Koizumi Schuichi、Yamazaki Maya、Abe Manabu、Sakimura Kenji、Yamashita Toshihide、Okamura Yasushi
  • Journal Title: Journal of Neurochemistry Volume: 142 Issue: 5 Pages: 686-699
  • DOI: 10.1111/jnc.14106
  • Peer Reviewed / Open Access
• [Journal Article] Retrograde BDNF to TrkB signaling promotes synapse elimination in the developing cerebellum. (2017)
  • Author(s): Choo M, Miyazaki T, Yamazaki M, Kawamura M, Nakazawa T, Zhang J, Tanimura A, Uesaka N, Watanabe M, Sakimura K, Kano M.
  • Journal Title: Nat Commun. Volume: 8(1) Issue: 1 Pages: 195-195
  • DOI: 10.1038/s41467-017-00260-w
  • Peer Reviewed / Open Access
• [Journal Article] Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1 (2017)
  • Author(s): Mashud Rana、Nomachi Akira、Hayakawa Akihide、Kubouchi Koji、Danno Sally、Hirata Takako、Matsuo Kazuhiko、Nakayama Takashi、Satoh Ryosuke、Sugiura Reiko、Abe Manabu、Sakimura Kenji、Wakana Shigeharu、Ohsaki Hiroyuki、Kamoshida Shingo、Mukai Hideyuki
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 7663-7663
  • DOI: 10.1038/s41598-017-07936-9
  • NAID: 120006366660
  • Peer Reviewed / Open Access
• [Journal Article] Drebrin E regulates neuroblast proliferation and chain migration in the adult brain. (2017)
  • Author(s): Kajita Y, Kojima N, Koganezawa N, Yamazaki H, Sakimura K, Shirao T.
  • Journal Title: Eur J Neurosci. Volume: 46 Pages: 2214-2228
  • Peer Reviewed
• [Journal Article] Netrin-1 Derived from the Ventricular Zone, but not the Floor Plate, Directs Hindbrain Commissural Axons to the Ventral Midline (2017)
  • Author(s): Yamauchi Kenta、Yamazaki Maya、Abe Manabu、Sakimura Kenji、Lickert Heiko、Kawasaki Takahiko、Murakami Fujio、Hirata Tatsumi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 11992-11992
  • DOI: 10.1038/s41598-017-12269-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potentiation of excitatory synaptic transmission ameliorates aggression in mice with Stxbp1 haploinsufficiency (2017)
  • Author(s): Miyamoto Hiroyuki、Shimohata Atsushi、Abe Manabu、Abe Teruo、Mazaki Emi、Amano Kenji、Suzuki Toshimitsu、Tatsukawa Tetsuya、Itohara Shigeyoshi、Sakimura Kenji、Yamakawa Kazuhiro
  • Journal Title: Human Molecular Genetics Volume: 26 Issue: 24 Pages: 4961-4974
  • DOI: 10.1093/hmg/ddx379
  • Peer Reviewed / Open Access
• [Journal Article] An RNA-binding protein, Qki5, regulates embryonic neural stem cells through pre-mRNA processing in cell adhesion signaling (2017)
  • Author(s): Hayakawa-Yano Yoshika、Suyama Satoshi、Nogami Masahiro、Yugami Masato、Koya Ikuko、Furukawa Takako、Zhou Li、Abe Manabu、Sakimura Kenji、Takebayashi Hirohide、Nakanishi Atsushi、Okano Hideyuki、Yano Masato
  • Journal Title: Genes & Development Volume: 31 Issue: 18 Pages: 1910-1925
  • DOI: 10.1101/gad.300822.117
  • Peer Reviewed / Open Access
• [Journal Article] Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex (2017)
  • Author(s): Hou X, Yoshioka N, Tsukano H, Sakai A, Miyata S, Watanabe Y, Yanagawa Y, Sakimura K, Takeuchi K, Kitagawa H, Hensch TK, Shibuki K, Igarashi M, Sugiyama S
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 12646-12646
  • DOI: 10.1038/s41598-017-04007-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mechanistic insight into the suppression of microglial ROS production by voltage-gated proton channels (VSOP/Hv1) (2017)
  • Author(s): Kawai Takafumi、Tatsumi Shoki、Kihara Shinji、Sakimura Kenji、Okamura Yasushi
  • Journal Title: Channels (Austin) Volume: 12 Issue: 1 Pages: 1-8
  • DOI: 10.1080/19336950.2017.1385684
  • Peer Reviewed / Open Access
• [Journal Article] Rufy3 is an adapter protein for small GTPases that activates a Rac guanine nucleotide exchange factor to control neuronal polarity (2017)
  • Author(s): Honda Atsuko、Usui Hiroshi、Sakimura Kenji、Igarashi Michihiro
  • Journal Title: Journal of Biological Chemistry Volume: 292 Issue: 51 Pages: 20936-20946
  • DOI: 10.1074/jbc.m117.809541
  • Peer Reviewed / Open Access
• [Journal Article] Orexin modulates behavioral fear expression through the locus coeruleus (2017)
  • Author(s): Soya Shingo、Takahashi Tohru M.、McHugh Thomas J.、Maejima Takashi、Herlitze Stefan、Abe Manabu、Sakimura Kenji、Sakurai Takeshi
  • Journal Title: Nature Communications Volume: 8 Issue: 1 Pages: 1606-1606
  • DOI: 10.1038/s41467-017-01782-z
  • NAID: 120007128779
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RNG105/Caprin1, an RNA granule protein for dendritic mRNA localization, is essential for long-term memory formation (2017)
  • Author(s): Kei Nakayama, Rie Ohashi, Yo Shinoda, Maya Yamazaki, Manabu Abe, Akihiro Fujikawa, Shuji Shigenobu, Akira Futatsugi, Masaharu Noda, Katsuhiko Mikoshiba, Teiichi Furuichi, Kenji Sakimura, and Nobuyuki Shiina
  • Journal Title: eLife Volume: 6 Pages: 1-32
  • DOI: 10.7554/elife.29677
  • Peer Reviewed / Open Access
• [Journal Article] Protocadherin-αC2 is required for diffuse projections of serotonergic axons (2017)
  • Author(s): Katori Shota、Noguchi-Katori Yukiko、Okayama Atsushi、Kawamura Yoshimi、Luo Wenshu、Sakimura Kenji、Hirabayashi Takahiro、Iwasato Takuji、Yagi Takeshi
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 15908-15908
  • DOI: 10.1038/s41598-017-16120-y
  • Peer Reviewed / Open Access
• [Journal Article] Maturation of Cerebellar Purkinje Cell Population Activity during Postnatal Refinement of Climbing Fiber Network. (2017)
  • Author(s): Good JM, Mahoney M, Miyazaki T, Tanaka KF, Sakimura K, Watanabe M, Kitamura K, Kano M.
  • Journal Title: Cell Rep. Volume: 21(8) Issue: 8 Pages: 2066-2073
  • DOI: 10.1016/j.celrep.2017.10.101
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Corrigendum: Conditional Knockout of Cav2.1 Disrupts the Accuracy of Spatial Recognition of CA1 Place Cells and Spatial/Contextual Recognition Behavior. (2017)
  • Author(s): Jung D, Hwang YJ, Ryu H, Kano M, Sakimura K, Cho J.
  • Journal Title: Front Behav Neurosci. Volume: 11 Pages: 242-242
  • DOI: 10.3389/fnbeh.2017.00242
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] C57BL/6系統マウスにおける卵管エレクトロポレーション法を用いた遺伝子編集効率の検討 (2019)
  • Author(s): 夏目里恵、中務胞、崎村建司、阿部学
  • Organizer: 第66回日本実験動物学会総会
• [Presentation] 卵管膨大部エレクトロポレーション法を用いた遺伝子組換えマウス作製の迅速化 (2018)
  • Author(s): 夏目里恵、阿部学、中務胞、崎村建司
  • Organizer: 第65回日本実験動物学会総会
• [Presentation] Extracerebellar expression of glutamate rexeptor GluD2 in adult mice and marmosets (2018)
  • Author(s): Watanabe M, Nakamoto C, Sakimura K, Kano M, Konno K
  • Organizer: 11th FENS Forum of Neuroscience
  • Int'l Joint Research
• [Presentation] Visualizing single-neuron identity specified by Pcdh-b cluster (2018)
  • Author(s): Kaneko R, Abe M, Takatsuru Y, Inoue YU, Watanabe M, Sakimura K, Yanagawa Y, Yagi T
  • Organizer: 第41回日本神経科学大会
• [Presentation] A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo (2018)
  • Author(s): Sakamoto M, Inoue M, Sakai K, Kobari S, Takemoto-Kimura S, Fujii H, Abe M, Sakimura K, Bito H
  • Organizer: 第41回日本神経科学大会
• [Presentation] Differential involvement of GluN2B and GluA1 in formation of left-right asymmetry in the hippocampus (2018)
  • Author(s): Kleindienst D, Kawakami R, Case MJ, Kobayashi K, Komiyama N, Abe M, Sakimura K, Shig Kawakami R, Case MJ, Kobayashi K, Komiyama N, Abe M, Sakimura K, Shigemoto R
  • Organizer: SfN's 48th Annual Meeting Neuroscience 2018
  • Int'l Joint Research
• [Presentation] 遺伝子改変ホモマウスへの過剰排卵処置方法の検討 (2018)
  • Author(s): 中務胞、夏目里恵、阿部学、崎村建司
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] The development of gene-manipulated animal models: aiming to generate better suited mouse, rat and marmoset for the analysis of brain function (2018)
  • Author(s): Kenji Sakimura
  • Organizer: The 8th BRI International Symposium
  • Invited
• [Presentation] CARD HyperOvaを用いたBNラットの過剰排卵誘起処置の有用性 (2017)
  • Author(s): 中務胞、夏目里恵、崎村建司
  • Organizer: 第64回日本実験動物学会総会
• [Presentation] GluD1・Cbln1複合体は分界条床核GABA作動性神経と結合腕傍核由来の神経終末との接着に関与する (2017)
  • Author(s): 今野幸太郎、森さくら、崎村建司、柚崎通介、渡辺雅彦
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] GABAニューロン前駆細胞の一部はグリア細胞に分化する (2017)
  • Author(s): 江角重行、柳川右千夫、崎村建司、鹿川哲史、石龍徳、竹林浩秀、玉巻伸章
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] D1/D2ドーパミン受容体コンディショナル発現マウスによる運動制御機構 (2017)
  • Author(s): 笹岡俊邦、佐藤朝子、知見聡美、大久保直、阿部学、川村名子、中尾聡宏、小田佳奈子、酒井清子、前田宜俊、神保幸弘、田中稔、山本美丘、佐藤俊哉、藤沢信義、崎村建司、南部篤
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 目的指向性行動における小脳モジュールの機能 (2017)
  • Author(s): 喜多村和郎、堤信一郎、日高直樹、磯村宜和、松崎正紀、崎村建司、狩野方伸
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 大脳皮質Arc経路活性化による遠隔記憶セルアンサンブルの制御 (2017)
  • Author(s): 金亮、酒井一輝、川島尚之、近藤弥生、井上昌俊、野中美応、坂本雅行、岡本理子、阿部学、崎村建司、今吉格、奥野浩行、尾藤晴彦
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 自閉症感受性遺伝子Auts2欠損による大脳神経細胞の過剰なシナプス形成の誘発 Loss of autism susceptibility gene, Auts2 induces the excessive synaptic formation in mouse forebrains. (2017)
  • Author(s): 山田 光代、堀 啓、坂本 亜沙美、阿部 学、崎村建司、曽根 雅紀、星野 幹雄
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 小脳におけるミクログリア依存的神経回路発達 Microglia Dependent Development of Neuronal Circuits in the Cerebellum (2017)
  • Author(s): 中山 寿子、阿部 学、森本 千恵、﨑村 建司、橋本 浩一
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] SRF転写活性化因子MKL2の機能解析：新規アイソフォームSOLOISTを中心として Functional analyses of SRF coactivator MKL2 including a novel isoform SOLOIST (2017)
  • Author(s): 田邉 広樹、石橋 悠太、庄司 しずく 、久保 友喜美、袴田 知之、田中 拓郎、森 寿、和泉 宏謙、崎村 健司 、福地 守、津田 正明、田渕 明子
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 網膜視細胞におけるCAST/ELKS欠損によって誘発される視機能異常の形態学的解析 CAST/ELKS deletion from photoreceptor causes blindness by synaptic and neural remodeling during retinal maturation (2017)
  • Author(s): 萩原 明 、北原 陽介、Vogl Christian、阿部 学、橘田 涼、太田 啓介、中村 桂一郎、崎村 建司、Moser Tobias、西 昭徳、大塚 稔久
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] CRF-Venus ΔNeoマウスの分界条床核外側部CRHニューロンのシナプス入力の性差ーホルマリンテストを用いて Sex specific changes in mEPSC of CRH neurons in the lateral BST in CRF-Venus ΔNeo mice in association with formalin test (2017)
  • Author(s): 萩原 裕子、崎村 建司、井樋 慶一、船橋 利也
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] プルキンエ細胞に発現するカルシニューリンB1サブユニットは抑制性シナプス形成過程において適切なプレ-ポストシナプスの接着に関わっている (2017)
  • Author(s): 宮﨑太輔、崎村建司、渡辺雅彦
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] ドレブリンはmGluR5およびＮＭＤＡ受容体依存性ＬＴＤ誘導に関わる (2017)
  • Author(s): 児島伸彦、安田浩樹、崎村建司、白尾智明
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 内因性カンナビノイド、2-アラキドノイルグリセロールはマウスのバレル皮質の視床皮質投射、4層-2/3層投射において重要な役割を果たす (2017)
  • Author(s): 木村文隆、伊丹千晶、崎村建司、狩野方伸
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Key roles of cannabinoid CB1 receptor and 2-arachidonoylglycerol in the regulation of excitatory synaptic transmission in the bed nucleus of the stria terminalis and neuropathic pain (2017)
  • Author(s): Ako Kato、Kazuto Sakoori, Kenji Sakimura, Yuki Sugaya, Masanobu Kano
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] BDNF-TrkBシグナルは 発達期小脳の登上線維シナプス刈り込みを制御する BDNF to TrkB signaling promotes climbing fiber synapse elimination in the developing cerebellum (2017)
  • Author(s): 秋 明貞、宮崎 太輔、山崎 真弥、川村 名子、中澤 敬信、 谷村 あさみ、上阪 直史、渡辺 雅彦、崎村 健司、狩野 方伸
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 小脳顆粒細胞特異的ニューレニシントリプルノックアウトマウスの作成と解析 Generation and analysis of cerebellar granule cell-specific neurexins triple knockout mice (2017)
  • Author(s): 植村 健、鈴木 絵美、小池 梨絵、川瀨 詩織、栗原 大河、崎村 建司、三品 昌美、田渕 克彦
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 臨界期における抑制性介在ニューロンのアクチン再構築の役割 A role of actin remodeling in cortical interneuron for critical period plasticity (2017)
  • Author(s): 侯 旭濱、國見 順子、崎村 建司、杉山 清佳
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] パルミトイル化サイト欠失型AMPA受容体ノックインマウスにおける興奮性神経活動の亢進と発作感受性の上昇 Disruption of AMPA receptor-palmitoylation leads excitatory/inhibitory imbalance and elevated seizure susceptibility (2017)
  • Author(s): 伊藤 政之、山下 真梨子、山田 大輔、奥野 浩行、阿部 学 、山崎 真弥、夏目 里恵、金子 雅規、貝塚 利恵、崎村 建司、関口 正幸、和田 圭司、星野 幹雄、三品 昌美、林 崇
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] オーファン代謝型受容体Prrt3の大脳特異的ノックアウトマウスの行動解析、およびそのリガンド同定に向けた小分子ライブラリーのスクリーニング Behavioral analyses of forebrain specific knock-out mice of an orphan metabotropic receptor Prrt3 and screening of small molecule library toward identification of its ligand (2017)
  • Author(s): 陳 以珊、山本 友美、周 麗、夏目 里恵、今野 幸太郎 、上杉 志成、渡辺 雅彦、高雄 啓三、宮川 剛、崎村建司、久保 義弘
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] GluD1欠損マウスにみられるうつ様行動は抗うつ薬により回復する Depression-like behavior in the GluD1 deficient mice was remedied by antidepressants (2017)
  • Author(s): 中本千尋、夏目 里恵、阿部 学、川村 名子、内田 仁司、渡辺 雅彦、崎村建司
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] マウス脳内のCAST/ELKSのリン酸化の生化学的解析 Biochemical analysis of phosphorylated CAST/ELKS in mice brain (2017)
  • Author(s): 浜田 駿、阿部 学、崎村 建司、大塚 稔久
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] ミクログリアに発現する電位依存性プロトンチャネルの脳内における発現差異とその機能 Gene expression and behavior analysis of microglial voltage-gated proton channels in mouse (2017)
  • Author(s): 河合 喬文、高雄 啓三、崎村建司、 宮川 剛 、岡村 康司
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 視床下部室傍核のCRFニューロンを投射部位により分類する Classification of corticotropin-releasing factor neurons in the hypothalamic paraventricular nucleus according to their differential neural projections (2017)
  • Author(s): 堀尾 修平、山形 聡、小林 憲太、加藤 成樹、崎村 建司、上山 敬司、小林 和人、井樋 慶一
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] コルチコトロピン放出因子（CRF）ニューロン選択的バゾプレシン（AVP）欠損マウスの作成：視床下部小細胞性ニューロンに発現するAVPの役割 Generation of corticotropin-releasing factor neuron-selective vasopressin knockout mouse: unravelling the physiological role of vasopressin expressed in the parvocellular neurons in the hypothalamus (2017)
  • Author(s): 山形 聡、Talukder Hossain Ashraf、渡辺 真秀、周 麗、夏目 里恵、阿部 学 、蔭山 和則、大門 眞、崎村 建司、井樋 慶一
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] オレキシンは青斑核ノルアドレナリンニューロンを介して恐怖情動表出を調節する Orexin modulates Behavioral Fear Expression through Noradrenergic Neurons in the Locus Coeruleus (2017)
  • Author(s): 高橋 徹、征矢 晋吾、Thomas J Mchugh、前島 隆、阿部 学、崎村 建司、櫻井 武
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 脊髄後角における神経障害性疼痛関連分子BEGAINの同定 Identification and characterization of brain-enriched guanylate kinase-associated protein as a neuropathic pain-related protein in the spinal dorsal horn (2017)
  • Author(s): 片野 泰代、福田 正史、山崎 真弥、 、阿部 学、渡辺 雅彦 、矢尾 育子、奥村 宣明、 中澤 敬信、山本 雅、崎村 建司、高尾 敏文、伊藤 誠二
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] 特異的カテプシンD欠損プルキンエ細胞の脆弱性 －特に、同Atg7欠損プルキンエ細胞との比較において Purkinje cells are more vulnerable to the specific depletion of cathepsin D than to that of Atg7 (2017)
  • Author(s): 小池 正人、柴田 昌宏、砂堀 毅彦 、山口 隼司、崎村 建司 、小松 雅明、田中 啓二 、内山 安男
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Maturation of cerebellar climbing fiber and Purkinje cell population activities during postnatal development (2017)
  • Author(s): Michael Mahoney、Jean-Marc Good、Taisuke Miyazaki、Kenji F Tanaka、Kenji Sakimura、Masahiko Watanabe、Kazuo Kitamura、 Masanobu Kano
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] マウス発生期海馬歯状回における自閉症感受性遺伝子AUTS2の役割 The role of Auts2 in the dentate gyrus development (2017)
  • Author(s): 江草 早紀、堀 啓、坂本 亜沙美、阿部 学、崎村 建司、郷 康広、星野 幹雄
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] ニューロンIDの可視化：クラスター型プロトカドヘリンの発現解析 Visualizing single-neuron identity specified by Pcdh-b cluster (2017)
  • Author(s): 金子 涼輔、阿部 学、高鶴 裕介、渡辺 雅彦、崎村 建司、柳川 右千夫、八木 健
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] NKによるGAP-43のリン酸化は軸索成長を制御する Phosphorylation of GAP-43 by JNK regulates axonal growth (2017)
  • Author(s): 河嵜 麻実、岡田 正康、 小林 大紀、野住 素広、 崎村 建司、仁科 博史、 武内 恒成、五十嵐 道弘
  • Organizer: 第40回日本神経科学大会
• [Presentation] 後天的セロトニン神経除去が睡眠覚醒に与える影響 Sleep/wakefulness behavior after ablation of central serotonergic neuron (2017)
  • Author(s): 岩崎 加奈子、柿崎 美代、阿部 学、崎村 建司、船戸 弘正、柳沢 正史
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] RNA編集酵素ADAR2は神経障害性疼痛に関与する RNA editing enzyme ADAR2 contributes to neuropathic pain (2017)
  • Author(s): 内田 仁司、崎村 建司、伊藤 誠二
  • Organizer: 第40回日本神経科学大会
  • Int'l Joint Research
• [Presentation] Depression-like behavior in the GluD1 deficient mice was remedied by antidepressants. (2017)
  • Author(s): Chihiro Nakamoto、Rie Natsume, Manabu Abe, Meiko Kawamura, Hitoshi Uchida, Masahiko Watanabe, Kenji Sakimura
  • Organizer: 26th Meeting of the International Society for Neurochemistry
  • Int'l Joint Research
• [Presentation] Roles of Arc/arg3.1 on surface expression dynamics of AMPA receptors during structural plasticity and on cognitive refinement processes (2017)
  • Author(s): H. OKUNO、Y. ISHII, T. ENDO, M. UEHARA, Y. SUZUKI, K. MINATOHARA, A. ARAKI, M. ABE, I. IMAYOSHI, M. KAKEYAMA, K. SAKIMURA, H. BITO
  • Organizer: Neuroscience2017
  • Int'l Joint Research
• [Presentation] Aif1-iCre knock-in mouse line: A tool for conditional gene manipulation in microglia (2017)
  • Author(s): M. ABE、F. PENG, K. SAKIMURA
  • Organizer: Neuroscience2017
  • Int'l Joint Research
• [Presentation] A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging In vivo (2017)
  • Author(s): M. SAKAMOTO、M. INOUE、K. SAKAI、 S. KOBARI、S. TAKEMOTO-KIMURA, M. ABE, K. SAKIMURA, H. BITO
  • Organizer: Neuroscience2017
  • Int'l Joint Research
• [Presentation] Rational engineering of XCaMPs, a multicolor GECI suite for In vivo imaging of brain circuit dynamics (2017)
  • Author(s): M. INOUE, A. TAKEUCHI, S. MANITA,I. HORIGANE1, R. KAWAKAMI, K. YAMAGUCHI, M. SAKAMOTO, H. YOKOYAMA, R. KIM, S. TAKEMOTO-KIMURA, M. ABE, K. SAKIMURA T. NEMOTO, M. KANO, H. FUJII, K. KITAMURA, H. BITO,
  • Organizer: Neuroscience2017
  • Int'l Joint Research
• [Presentation] ラット卵巣卵胞内卵子の成熟培養なしでの体外受精 (2017)
  • Author(s): Neuroscience2017
  • Organizer: 第40回日本分子生物学会年会
  • Int'l Joint Research
• [Presentation] 発生および慢性疼痛下の一次知覚神経におけるRNA編集の変動 (2017)
  • Author(s): 内田 仁司、松村伸治、崎村建司、伊藤 誠二
  • Organizer: 第40回日本分子生物学会年会
  • Int'l Joint Research
• [Presentation] ニューロンIDの可視化 ～クラスター型プロトカドヘリンの発現解析～ (2017)
  • Author(s): 金子 涼輔、阿部学、高鶴裕介、渡辺雅彦、崎村建司、柳川 右千夫、八木 健
  • Organizer: 第40回日本分子生物学会年会
  • Int'l Joint Research
• [Presentation] PKN1はリンパ球の細胞運動・トラフィッキングを制御する (2017)
  • Author(s): 窪内 康二、團野紗莉、野町昭、平田多佳子、松尾一彦、中山隆志、佐藤亮介、杉浦麗子、阿部学、崎村建司、若菜茂晴、大崎博之、鴨志田伸吾、向井 秀幸
  • Organizer: 第40回日本分子生物学会年会
  • Int'l Joint Research
• [Presentation] Ｄ１/Ｄ２ドーパミン受容体コンディショナル発現マウスによる運動制御機構の解明 (2017)
  • Author(s): 笹岡 俊邦、佐藤朝子、知見聡美、大久保直、阿部学、川村名子、中尾聡宏、齊藤奈英、酒井清子、小田佳奈子、前田宜俊、神保幸弘、田中稔、山本美丘、佐藤俊哉、藤澤信義、崎村建司、南部篤
  • Organizer: 第40回日本分子生物学会年会
  • Int'l Joint Research
• [Remarks] 新潟大学脳研究所Webページ
  • URL: http://www.bri.niigata-u.ac.jp/