| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
We prapered adeno-associated virus vectors with capsid insertions of various mutations (AAV2/X), and found that the infection efficiency varied greatly depending on the neuron type. In addition, by replacing the amino acids in the spike protruding from the surface of the viral particles, we succeeded in reducing the infection efficiency significantly in vitro. We further examined the infection efficiency in the mouse brain in vivo, and failed to suppress non-specific infection completely. To achieve more specific infections, the non-specific infection of virus vectors should be further suppressed.
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