| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Outline of Final Research Achievements |
Abstract of the current study is summarized as follows. 1. Histological analysis revealed that mouse ventral striatum included the islands of Calleja expressing Gad67 mRNA, NeuN-positive GABAergic neurons. 2. A novel behavioral system for quantitative measurement of anhedonia-like behaviors was developed for mice. Specifically, sequential sweep of the descending stimulation frequencies for the intracranial self-stimulation upon nose poking of the port in the operant chamber enabled the quantification of the behavioral measure useful for anhedonia-like behaviors. Indeed, antidepressant nomifensine decreased significantly threshold for the hedonic behavior revealed by the current methods. 3. Gene expression sets specific to the murine islands of Calleja was established by applying immunoprecipitation and RNA-seq analyses to the mouse line expressing ribosomal protein-GFP fusion protein specifically in the islands of Calleja.
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