| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Outline of Final Research Achievements |
We previously developed tailed duplexes, several hundred-base single-stranded DNAs with an oligodeoxyribonucleotide annealed to the 3'-region of the long ss DNAs. In this study, we examined various approaches towards genome editing with tailed duplexes. We found that (i) editing efficiency was enhanced when a second mismatch was introduced into the tailed duplexes and that (ii) tailed duplexes with nearly 100-base single-stranded DNAs and with the single strand and double strand junctions close to the target positions showed high editing efficiencies. We also demonstrated that (iii) genome editing was achieved by the tailed duplex in cultured cells, without artificial nucleases.
|
