| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
It has been suggested that the amount of oxidative nucleoside damage in DNA due to stress and reactive oxygen species is related with various diseases. However, there is no general method for sequence specific detection of oxidative damaged nucleobases in DNA. Therefore, we have designed a new artificial nucleoside analogues and succeeded in chemical synthesis. Also, we have succeeded in developing new technology capable of recognizing and detecting the oxidative damaged nucleobases using artificial nucleoside analogues and DNA polymerase. In addition, the results of this study suggesting that the presence of oxidative damaged nucleobases may be involved in transversion mutations that may be involved in carcinogenesis were obtained.
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