Configuration of cell axes for the development of cell authentication system
| Project/Area Number |
17K19507
|
|---|
| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biomedical structure and function and related fields
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
Ohba Yusuke 北海道大学, 医学研究院, 教授 (30333503)
|
|---|
| Project Period (FY) |
2017-06-30 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
|
|---|
| Keywords | 細胞形態 / 細胞内小器官 / 細胞認証 |
|---|
| Outline of Final Research Achievements |
In order to set a "template" for authenticating cells and a "coordinate axis" for describing it, parameters regarding the arrangement of each organelle were obtained. For this purpose, an organelle marker library was constructed using various fluorescent proteins. It was found that the facilitation of the folding of fluorescent proteins might negatively affect the localization of the organelle markers. In addition, by analyzing the arrangement of organelles using the library, it was found that the centroids of the endosomal system formed a certain line in cells.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究成果により、これまで経験則にのみ基づいていた細胞の形態認識に客観的な指標を与え、生物を題材とした研究ゆえのheterogeneityを説明するような知見が提供されることが期待できる。
|
Report
(3 results)
Research Products
(25 results)
-
[Journal Article] A Peptide Derived from Phosphoinositide 3-kinase Inhibits Endocytosis and Influenza Virus Infection2019
Author(s)
Fujioka Y, Satoh AO, Horiuchi K, Fujioka M, Tsutsumi K, Sasaki J, Nepal P, Kashiwagi S, Paudel S, Nishide S, Nanbo A, Sasaki T, Ohba Y
-
Journal Title
Cell Structure and Function
Volume: 44
Issue: 1
Pages: 61-74
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells2018
Author(s)
Fujioka Y, Nishide S, Ose T, Suzuki T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh AO, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Sato Y, Paudel S, Nanbo A, Miyazaki T, Hasegawa H, Maenaka K, Ohba Y
-
Journal Title
Cell Host Microbe
Volume: 23
Issue: 6
Pages: 809-818
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
[Journal Article] Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells.2017
Author(s)
S. Saitoh, T. Maruyama, Y. Yako, M. Kajita, Y. Fujioka, Y. Ohba, N. Kasai, N. Sugama, S. Kon, S. Ishikawa, T. Hayashi, T. Yamazaki, M. Tada and Y. Fujita.
-
Journal Title
Proc. Natl. Acad. Sci. U.S.A.
Volume: 114
Issue: 12
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
