Configuration of cell axes for the development of cell authentication system

• Project/Area Number: 17K19507
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical structure and function and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Ohba Yusuke 北海道大学, 医学研究院, 教授 (30333503)
• Project Period (FY): 2017-06-30 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 細胞形態 / 細胞内小器官 / 細胞認証

Outline of Final Research Achievements

In order to set a "template" for authenticating cells and a "coordinate axis" for describing it, parameters regarding the arrangement of each organelle were obtained. For this purpose, an organelle marker library was constructed using various fluorescent proteins. It was found that the facilitation of the folding of fluorescent proteins might negatively affect the localization of the organelle markers. In addition, by analyzing the arrangement of organelles using the library, it was found that the centroids of the endosomal system formed a certain line in cells.

Academic Significance and Societal Importance of the Research Achievements

本研究成果により、これまで経験則にのみ基づいていた細胞の形態認識に客観的な指標を与え、生物を題材とした研究ゆえのheterogeneityを説明するような知見が提供されることが期待できる。

Research Products

• [Journal Article] A Peptide Derived from Phosphoinositide 3-kinase Inhibits Endocytosis and Influenza Virus Infection (2019)
  • Author(s): Fujioka Y, Satoh AO, Horiuchi K, Fujioka M, Tsutsumi K, Sasaki J, Nepal P, Kashiwagi S, Paudel S, Nishide S, Nanbo A, Sasaki T, Ohba Y
  • Journal Title: Cell Structure and Function Volume: 44 Issue: 1 Pages: 61-74
  • DOI: 10.1247/csf.19001
  • NAID: 130007636814
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells (2018)
  • Author(s): Fujioka Y, Nishide S, Ose T, Suzuki T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh AO, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Sato Y, Paudel S, Nanbo A, Miyazaki T, Hasegawa H, Maenaka K, Ohba Y
  • Journal Title: Cell Host Microbe Volume: 23 Issue: 6 Pages: 809-818
  • DOI: 10.1016/j.chom.2018.04.015
  • NAID: 120006643588
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The Role of Transforming Growth Factor β in Cell-to-Cell Contact-Mediated Epstein-Barr Virus Transmission (2018)
  • Author(s): Nanbo A, Ohashi M, Yoshiyama H, Ohba Y
  • Journal Title: Front Microbiol Volume: 9 Pages: 984-984
  • DOI: 10.3389/fmicb.2018.00984
  • NAID: 120006489803
  • Peer Reviewed / Open Access
• [Journal Article] Infection of Epstein?Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines (2018)
  • Author(s): Nanbo Asuka、Katano Harutaka、Kataoka Michiyo、Hoshina Shiho、Sekizuka Tsuyoshi、Kuroda Makoto、Ohba Yusuke
  • Journal Title: Cancers Volume: 10 Issue: 7 Pages: 237-237
  • DOI: 10.3390/cancers10070237
  • NAID: 120006491456
  • Peer Reviewed / Open Access
• [Journal Article] Budding of Ebola Virus Particles Requires the Rab11-Dependent Endocytic Recycling Pathway (2018)
  • Author(s): Nanbo A, Ohba Y
  • Journal Title: J Infect Dis Volume: 218 Issue: suppl_5 Pages: S388-S396
  • DOI: 10.1093/infdis/jiy460
  • NAID: 120006773327
  • Peer Reviewed / Open Access
• [Journal Article] Epstein-Barr Virus Acquires Its Final Envelope on Intracellular Compartments With Golgi Markers (2018)
  • Author(s): Nanbo Asuka、Noda Takeshi、Ohba Yusuke
  • Journal Title: Front Microbiol Volume: 9 Pages: 454-454
  • DOI: 10.3389/fmicb.2018.00454
  • NAID: 120006416029
  • Peer Reviewed / Open Access
• [Journal Article] Dermoscopic evaluation for skin grafts after surgery; neo-vascularization correlates with survival of skin grafts: A prospective study (2018)
  • Author(s): Kitamura Shinya、Yanagi Teruki、Inamura-Takashima Yuka、Imafuku Keisuke、Hata Hiroo、Fujioka Yoichiro、Ohba Yusuke、Shimizu Hiroshi
  • Journal Title: J Dermatol Sci Volume: 印刷中 Issue: 2 Pages: 213-216
  • DOI: 10.1016/j.jdermsci.2018.01.014
  • Peer Reviewed
• [Journal Article] Ebola virus requires a host scramblase for externalization of phosphatidylserine on the surface of viral particles (2018)
  • Author(s): Nanbo Asuka、Maruyama Junki、Imai Masaki、Ujie Michiko、Fujioka Yoichiro、Nishide Shinya、Takada Ayato、Ohba Yusuke、Kawaoka Yoshihiro
  • Journal Title: PLoS Pathog Volume: 14 Issue: 1 Pages: e1006848-e1006848
  • DOI: 10.1371/journal.ppat.1006848
  • NAID: 120006416019
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cell competition with normal epithelial cells promotes apical extrusion of transformed cells through metabolic changes. (2017)
  • Author(s): Shunsuke Kon, et al.
  • Journal Title: Nature Cell Biology Volume: 印刷中 Issue: 5 Pages: 530-541
  • DOI: 10.1038/ncb3509
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rab5-regulated endocytosis plays a crucial role in apical extrusion of transformed cells. (2017)
  • Author(s): S. Saitoh, T. Maruyama, Y. Yako, M. Kajita, Y. Fujioka, Y. Ohba, N. Kasai, N. Sugama, S. Kon, S. Ishikawa, T. Hayashi, T. Yamazaki, M. Tada and Y. Fujita.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 114 Issue: 12
  • DOI: 10.1073/pnas.1602349114
  • NAID: 120006346108
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Fluorescence Imaging of membrane dynamics and intracellular signaling (2019)
  • Author(s): Yusuke Ohba
  • Organizer: The 9th Federation of Asian and Oceanian Physiological Societies Congress (FAOPS2019) in conjunction with the 96th Annual Meeting of the Physiological Society of Japan
• [Presentation] A PI3K-derived peptide inhibits clathrin-independent endocytosis and influenza virus infection (2018)
  • Author(s): Yoichiro Fujioka, Aya O Satoh, Kosui Horiuchi, Mari Fujioka, Sarad Paudel, Asuka Nanbo, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] Involvement of actin dynamics in the endocytic process revealed by fast-scanning atomic force microscopy (2018)
  • Author(s): Aiko Yoshida, Nobuaki Sakai, Yoshitsugu Uekusa, Shige H Yoshimura, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] The role of an Na,K-ATPase in spatiotemporal regulation of Ras- PI3K signaling and endocytosis (2018)
  • Author(s): Sayaka Kashiwagi, Yoichiro Fujioka, Kosui Horiuchi, Aya O Satoh, Prabha Nepal, Aiko Yoshida, Sarad Paudel, Asuka Nanbo, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] A mitochondrial outer membrane protein is involved in the regulation of Ras-PI3K signaling-mediated endocytosis (2018)
  • Author(s): Aya O Satoh, Yoichiro Fujioka, Kosui Horiuchi, Prabha Nepal, Sayaka Kashiwagi, Aiko Yoshida, Mari Fujioka, Sarad Paudel, Asuka Nanbo, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] Development of FRET-based biosensors for measuring tyrosine kinase activity in living cells (2018)
  • Author(s): Mari Fujioka, Yoichiro Fujioka, Aya O Satoh, Prabha Nepal, Sayaka Kashiwagi, Aiko Yoshida, Sarad Paudel, Asuka Nanbo, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] The Role of tubulin in the regulation of endocytosis mediated by Ras-PI3K signaling (2018)
  • Author(s): Sarad Paudel, Yoichiro Fujioka, Aya O. Satoh, Mari Fujioka, Kosui Horiuchi, Prabha Nepal, Sayaka Kashiwagi, Aiko Yoshida, Asuka Nanbo, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] Role of inner mitochondrial membrane proteins in the regulation of endocytosis mediated by Ras-PI3K signaling (2018)
  • Author(s): Prabha Nepal, Yoichiro Fujioka, Aya O. Satoh, Kosui Horiuchi, Sarad Paudel, Sayaka Kashiwagi, Aiko Yoshida, Mari Fujioka, Asuka Nanbo, Yusuke Ohba
  • Organizer: 第70回日本細胞生物学会
• [Presentation] 細胞内シグナル伝達の可視化と光による操作 (2017)
  • Author(s): 大場雄介
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] PI3K 由来ペプチドによるエンドサイトーシスとインフルエンザウイルス感染の抑制 (2017)
  • Author(s): 藤岡容一朗、佐藤絢、堀内浩水、藤岡真理、パウデル サラド、西出真也、南保明日香、大場雄介
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] 高速原子間力顕微鏡を用いた細胞表面膜構造動態のライブセルイメージング (2017)
  • Author(s): 吉田藍子、酒井信明、植草良嗣、粂田昌宏、吉村成弘、大場雄介
  • Organizer: 第40回日本分子生物学会年会
• [Presentation] インフルエンザウイルス細胞侵入において鍵となる宿主タンパク質の同定 (2017)
  • Author(s): 藤岡容一朗、西出真也、尾瀬農之、加藤いづみ、福原秀雄、藤岡真理、堀内浩水、佐藤絢、Prabha Nepal、柏木彩花、Jing Wang、堀口美香、Sarad Paudel、南保明日香、宮崎忠昭、前仲勝実、大場雄介
  • Organizer: 第69回日本細胞生物学会
• [Presentation] Ras-PI3K複合体によるエンドサイトーシスの制御因子の探索と機能解析 (2017)
  • Author(s): 堀内浩水、藤岡容一朗、佐藤絢、Prabha Nepal、Jing Wang、堀口美香、Sarad Paudel、西出真也、南保明日香、小布施力史、大場雄介
  • Organizer: 第69回日本細胞生物学会
• [Presentation] PI3K 由来ペプチドによるインフルエンザウイルス感染の抑制 (2017)
  • Author(s): 佐藤絢、藤岡容一朗、堀内浩水、藤岡真理、パウデル サラド、西出真也、南保明日香、大場雄介
  • Organizer: 第69回日本細胞生物学会
• [Book] Methods in Molecular Biology, SH2 domain (2017)
  • Author(s): Mari Fujioka、Yumi Asano、Shigeyuki Nakada、Yusuke Ohba
  • Total Pages: 555
  • Publisher: Humana Press, New York, NY