Modulating immunological memory of antibody-producing cells

Research Project

Project/Area Number	17K19539
Research Category	Grant-in-Aid for Challenging Research (Exploratory)
Allocation Type	Multi-year Fund
Research Field	Pathology, Infection/Immunology, and related fields
Research Institution	Tohoku University
Principal Investigator	TAKAI Toshiyuki 東北大学, 加齢医学研究所, 教授 (20187917)
Project Period (FY)	2017-06-30 – 2020-03-31
Project Status	Completed (Fiscal Year 2019)
Budget Amount *help	¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000) Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000) Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	免疫記憶 / 免疫寛容・自己免疫 / 免疫シグナル伝達 / 炎症 / 免疫制御・移植免疫
Outline of Final Research Achievements	We established an experimental system composed of culturing murine bone marrow-derived mesenchymal stem cells (MSC) and plasma cells (PC) in vitro and monitoring antibody production levels after the 7-day culture. We found that the antibody production by PC was influenced by IL-6 and other soluble factors secreted by MSC and unidentified direct interaction between MSC and PC. We also suggested that the direct interaction involves binding between an immunoregulatory receptor LILRB4 (B4) on PC and a novel B4 ligand, B4L1, expressed on MSC.
Academic Significance and Societal Importance of the Research Achievements	自然感染やワクチン接種により得られる終生免疫ないし長期的な免疫の維持機構はよくわかっていない。況や，この免疫記憶の調節技術も開発されていない。免疫記憶の維持が行われている場は主に骨髄と考えられ，骨髄中での抗体産生細胞由来の記憶細胞と骨髄中の細胞の相互作用に着目して得られた本研究成果は抗体産生の維持機構，免疫記憶の機構を理解し，その維持，改変技術の開発に向けた基盤となるもので，よりよいワクチン開発に資するものである。